Estudo da detecção de Salmonella spp. em alimentos comercializados na cidade de Fortaleza-CE através de método molecular (qPCR) e método convencional

Francisca Raquel Vieira de Araújo; Cícera Nayara Alexandre de Oliveira; Sônia Coelho Abreu de Oliveira; Ticiane Coelho Abreu de Oliveira; Cyntia Ladyane Alves de  Moura; Mayra Garcia Maia Costa

doi:10.59171/nutrivisa-2024v11e12262

Authors

Francisca Raquel Vieira de Araújo Núcleo de Tecnologia e Qualidade Industrial do Ceará – NUTEC https://orcid.org/0009-0003-7909-9620
Cícera Nayara Alexandre de Oliveira Núcleo de Tecnologia e Qualidade Industrial do Ceará – NUTEC https://orcid.org/0009-0003-7909-9620
Sônia Coelho Abreu de Oliveira Núcleo de Tecnologia e Qualidade Industrial do Ceará – NUTEC https://orcid.org/0009-0005-7757-5165
Ticiane Coelho Abreu de Oliveira Núcleo de Tecnologia e Qualidade Industrial do Ceará – NUTEC https://orcid.org/0009-0009-4078-7120
Cyntia Ladyane Alves de Moura Núcleo de Tecnologia e Qualidade Industrial do Ceará – NUTEC https://orcid.org/0009-0002-5701-0034
Mayra Garcia Maia Costa Núcleo de Tecnologia e Qualidade Industrial do Ceará – NUTEC https://orcid.org/0000-0001-7951-997X

DOI:

https://doi.org/10.59171/nutrivisa-2024v11e12262

Keywords:

Salmonella spp., qPCR, food molecular methods

Abstract

Salmonellosis is one of the most common diseases in the world, transmitted by food contaminated with different serotypes of Salmonella spp., which threaten food safety and public health. In many countries the Enteritides and Thyphtmurium sovars are the most common, occurring mainly in eggs, chicken and meat. Food can be contaminated with this microorganism throughout the entire production chain, during the transport, processing, storage, distribution and distribution stages. Currently, there are several methodologies for detecting Salmonella spp., including conventional isolation, rapid tests and rapid molecular methodologies. The quantitative polymerase chain reaction (qPCR) or real-time PCR method is a promising tool in studies that aim to quantify the population of microorganisms associated with food and has the advantage of detecting microorganisms even in small concentrations in the sample. In this research, 182 food samples of animal and vegetable origin, sold in the city of Fortaleza-CE, were subjected to an analysis to detect Salmonella spp. compare two different methodologies used to detect these microorganisms (conventional isolation technique - ISO 6579 and real-time PCR (Gene UP Platform). Of the samples tested, Salmonella spp. were detected in 38 samples using real-time PCR (Gene up ) and in 22 samples using the conventional isolation technique - ISO 6579, representing detection percentages of 20% and 12%, respectively. The difference of 8% positivity between the methods is justified by the qPCR technique being a method that detects in molecular level of microbial DNA, presenting high sensitivity, specificity, precision and speed. While the traditional conventional isolation test detects the microorganism at cultivable levels, that is, one that is capable of developing in food. It was found that the molecular method used is a promising tool for diagnosing pathogens in food and can optimize assay response times in laboratories.

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