Published 2023-01-16
Keywords
- Sperm,
- CASA,
- Squirrel monkey,
- Biometry
How to Cite
This work is licensed under a Creative Commons Attribution 4.0 International License.
Abstract
The objective was to evaluate the sperm morphometry between normal and pathological cells in Saimiri cassiquiarensis aiming at the future use of data in CASA (Computer Assisted Sperm Analysis). Semen was collected from a male (S. cassiquiarensis) by electroejaculation. After collection, a smear in the proportion of 1: 1 (semen and eosin-nigrosin stain) was performed, and 100 sperm were measured and morphologically classified as normal and pathological defects (major and minor). Of the total sperm analyzed, 48% were classified as normal and 52% pathological. Of the pathologies, the most frequent was a folded tail (40%), followed by a strongly folded tail (5%), curled tail (2%) and isolated head (5%). The morphometric data showed no statistical difference (p<0.05) between normal and pathological defects. This is the first description for the sperm morphometry in Saimiri cassiquiarensis, therefore, from this database, future assessments at CASA can be conducted for this species.
Downloads
References
- LYNCH ALFARO, J.W.L.; BOUBLI, J.P.; PAIM, F.P.; RIBAS, C.C.; SILVA, M.N.F.; MESSIAS, M.R.; ROHE, F.; MERCES, M.P.; SILVA JUNIOR, J.S.; SILVA, C.R.; PINHO, G.M.; KOSHKARIAN, G.; NGUYEN, M.T.; HARADA, M.L.; RABELO, R.M.; QUEIROZ, H.L.; ALFARO, M.E.; FARIAS, I.P. Biogeography of squirrel monkeys (genus Saimiri): South-central Amazon origin and rapid pan-Amazonian diversification of a lowland primate. Molecular Phylogenetics and Evolution, v.82, p.436–54, 2015.
- GARCÍA, D.A.; MONTIEL, J.L.C.; PÉREZ, O.H.; PICHARDO, E.M.; REZA, F.A.C.; GARCÍA, R.A. Estudio comparativo de los testículos, epidídimos,glândulas sexuales accesorias y espermatozoides en tres especies de lagomorfos (Romerolagos diazi, Lepus californicus y Oryctolagus cuniculus). Acta Zoológica Mexicana, v.88, p.257–69, 2003.
- MATOS, D.L.; ARAÚJO, A.A.; ROBERTO, I.G.; TONIOLLI, R. Análise computadorizada de espermatozoides: revisão de literatura. Revista Brasileira de Reprodução Animal, v.32, n.4, p.225-232, 2008.
- OLIVEIRA, K.G.; SANTOS, R.R.; LEÃO, D.L.; QUEIROZ, H.L.; PAIM, F.P.; VIANEZ-JÚNIOR, J.L.S.G; DOMINGUES, S.F.S. Testicular biometry and semen characteristics in captive and wild squirrel monkey species (Saimiri sp.). Theriogenology, v.86, n.3, p.879–87, 2016.
- OLIVEIRA, K.G.; LEÃO, D.L.; SANTOS, R.R.; DOMINGUES, S.F.S. Seminal characteristics and cryopreservation of sperm from the squirrel monkey, Saimiri collinsi. Theriogenology, v.85, n.5, p.743-749, 2015.
- VERSTEGEN, J.; IGUER-OUADA, M.; ONCLIN, K. Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, v.57, n.1, p.149-179, 2002.
- STEINBERG, E.R.; NIEVES, M.; ASCUNCE, M.S.; PALERMO, A.M.; MUDRY, M.D. Morphological and genetic characterization of Saimiri boliviensis. International Journal of Primatology, v.30, p.29–41, 2009.
- SAMPAIO, W.V.; OLIVEIRA, K.G.; LEÃO, D.L.; CALDAS-BUSSIERE, M.C.; QUEIROZ, H.L.; PAIM, F.P.; DOMINGUES, S.F.S. Morphologic analysis of sperm from two Neotropical primate species: Comparisons between the squirrel monkeys Saimiri collinsi and Saimiri vanzolinii. Zygote, v.25, n.1, p.141-148, 2017a.
- SAMPAIO, W.V.; LEÃO, D.L.; SOUSA, P.C.; DOMINGUES, S.F.S. Contribuições da biotecnologia da reprodução sobre os desafios na manipulação do sêmen de primatas do gênero Saimiri. Revista Brasileira de Reprodução Animal, v.41, n.1, p.222-230, 2017b.