IMPACTO DE ANTIMICROBIANOS NOS PARÂMETROS CINÉTICOS DE ESPERMATOZOIDES CRIOPRESERVADOS DE CATETOS
Palavras-chave:
Biobank, semen, cryopreservation, antimicrobialsResumo
The objective was to verify the impact of the addition of antimicrobials on the kinetic parameters of sperm in the cryopreserved semen of collared peccaries. Ejaculates from 10 adult male, obtained by electroejaculation, were used. The samples had their kinetic parameters evaluated by computer analysis (CASA). Subsequently, they were cryopreserved in Tris plus egg yolk (20%) and glycerol (3%), whether or not(control) added gentamicin (70μg/mL) or the combination penicillin (1000 IU/mL) and streptomycin (1mgE/mL) (P+E). After one week, the samples were thawed and evaluated similarly to fresh semen. In fresh semen, total motility of 95.3±0.8% and 72.1±3.5% progressive motility were observed. After thawing, there were no differences between treatments, except for the cross-beat frequency (BCF) parameter, which was negatively influenced by P+E, in relation to fresh semen (p<0.05). In conclusion, it is suggested the use of gentamicin as an antimicrobial for the cryopreservation of semen from peccaries.
Referências
AMANN, R.; KATZ, D.F. Reflections on CASA after 25 years. Journal of Andrology, v.25, n.3, p.317-325, 2004.
ANDRABI, S.M.H., KHAN, L.A., SHAHAB, M. Isolation of bacteria in semen and evaluation of antibiotics in extender for cryopreservation of buffalo (Bubalus bubalis) bull spermatozoa. Andrologia,v.48, n.10, p.1166-1174, 2016.
CASTELO, T.S.; BEZERRA, F.S.B.; SOUZA, A.L.P.; MOREIRA, M.A.P.; PAULA, V.V.; OLIVEIRA, M.F.; SILVA, A.R. Influence of the thawing rate on the cryopreservation of semen from collared peccaries (Tayassu tajacu) using tris-based extenders. Theriogenology, v.74, n.6, p.1060-1065, 2010.
GIL, M.C.; GARCÍA-HERREROS, M.; BARÓN, F.J.; APARICIO, I.M.; SANTOS, A.J.; GARCÍA-MARÍN, L.J. Morphometry of porcine spermatozoa and its functional significance in relation with the motility parameters in fresh semen. Theriogenology, v.71, n.2, p.254-263, 2009.
GONGORA, J.; REYNA-HURTADO, R.; BECK, H.; TABER, A.; ALTRICHTER, M.; KEUROGHLIAN, A. 2011. Pecari tajacu. The IUCN Red List of Threatened Species 2011:e.T41777A10562361.http://dx.doi.org/10.2305/IUCN.UK.20112.RLTS.T41777A10562361.en. Downloaded on 13 August 2018.
RURANGWA, E.; KIME, D. E.; OLLEVIER, F.; NASH, J.P. The measurement of sperm motility and factors affecting sperm quality in cultured fish. Review article. Aquaculture, Amsterdam, v.234, n.1/4, p.1-28, 2004.
SOUZA, A.L.P.; LIMA, G.L.; PEIXOTO, G.C.X.; SILVA, A.M.; OLIVEIRA, M.F.; SILVA, A.R. Use of Aloe vera-based extender for chilling and freezing collared peccary (Pecari tajacu) semen. Theriogenology, v.85, n.8, p.1432-1438, 2016.
TORTORA, G.J.; FUNKE, B.R.; CASE, C.L. Microbiologia. 12ª ed., Porto Alegre: Artmed, 2017. 962p.
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.