Boar semen motility, vigour and acrosomal quality in diferents extender after thawing
Keywords:
Semen, diluent, conservation, boarAbstract
ABSTRACT
The objective of this study was to determine a diluent that would provide good maintenance of the boar's semen viability after thawing. The following diluents were used: Beltsville Thawing Solution (BTS), BTS + 3-indole acetic acid (IAA) and Androhep (ADHP). The 24 ejaculates were analyzed in natura and after dilution, regarding sperm vigor and motility and acrosome integrity. Then, a total of 10,2 x109 spermatozoa were removed from each ejaculate, which were equally distributed among the different experimental treatments. The ejaculates were diluted in equal parts (1:1) at 30 oC and incubated for 2 hours before starting the freezing curve. Subsequently the semen was packed in 0,5 mL vats, at a concentration of 112 x106 spermatozoa/mL, submitted to nitrogen vapor for 4 minutes and then immersed in liquid nitrogen (-196 oC). Samples were thawed in a water bath at (37 °C/30s), then the contents were resuspended in the different diluents (1:5). The samples were evaluated as many as the same characteristics of semen in natura. In the statistical analysis, the Mann-Whitney tests were used, with a confidence interval of 5%. The BTS diluent had better sperm motility and vigor compared to ADPH in 5 minutes of incubation, but there were no significant differences on the same evaluations when the treatments were incubated for 1 hour. The acrosomal integrity of the ADPH diluent provided a higher number of intact spermatozoa (p<0.05) in relation to the other treatments. A extender that favors the conservation of a better sperm quality after thawing, still needs to be developed.
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