Publicado 2021-12-31
Palavras-chave
- Espermatozoide,
- lesões,
- membrana plasmática,
- proteínas
Como Citar

Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Resumo
A criopreservação induz mudanças estruturais e funcionais nos espermatozoides, que podem variar entre animais da mesma espécie, mesmo quando o sêmen deles é submetido ao mesmo protocolo de criopreservação. Essa característica leva alguns estudos a questionarem quanto à composição protéica do sêmen e sua influência na proteção espermática, em relação ao choque térmico. O plasma seminal de suínos apresenta predominância de espermadesinas, que são proteínas de baixo peso molecular e apresentam função de proteção da membrana plasmática. A criopreservação é o método mais eficiente para a conservação de espermatozoides a longo prazo. No entanto, o uso do sêmen congelado na espécie suína ainda está associado a baixos índices produtivos, pois o processo de criopreservação ocasiona diversos danos ao espermatozoide. Estudos têm sido realizados com a finalidade de identificar as causas específicas desses danos, a fim de promover uma melhor proteção ao espermatozoide criopreservado. Dessa forma, o presente trabalho tem como objetivo abordar os principais componentes moleculares do sêmen, que estão envolvidos no processo de criopreservação.
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Referências
- ALBERTS, B.; JOHNSON, A.; LEWIS, J.; MORGAN, D.; RAFF, M.; ROBERTS, K.; WALTER, P.; WILSON, J.; HUNT, T. Organização interna da célula: Estrutura da membrana. In: ___. Biologia Molecular da Célula. 4ª ed., Porto Alegre: Artmed. Cap. 10, p.583-595, 2004.
- ALMEIDA, J.L. Efeito de diferentes concentrações de plasma seminal na criopreservação do sêmen equino. 2006. 75p. (Dissertação). Brasília: Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília; 2006.
- AMANN, R.P.; GRAHAM, J.K. Spermatozoa function. In: McKINNON, A.O.; VOSS, J.L. (Ed.) Equine Reproduction. 2a ed., Philadelphia: Lea &Febiger, p.715-745, 1993.
- ANDRADE, A.F.C.; PEDROSA, A.C.; PASSARELLI, M.S.; MARTINS, S.M.M.K. Protocolos e possibilidades de criopreservação de sêmen suíno. Revista Brasileira de Reprodução Animal, v.43, n.2, p.89-96, 2019.
- AZEVEDO, H.C.; MACHADO, R.; SIMPLÍCIO, A.A.; SOARES, A.T. Características do sêmen caprino congelado: influência do tipo de palheta e concentração espermática. Revista Ciência Rural, v.5, n.2, p.148-157, 2000.
- BAILEY, J.L.; LESSARD, C.; JACQUES, J.; BRÈQUE, C.; DOBRINSKI, I.; ZENG, W.; GALANTINO-HOMER, H.L. Cryopreservation of boar semen and its future importance to the industry. Theriogenology, v.70, p.1251–1259, 2008.
- BOERKE, A.; DIELEMAN, S.J.; GADELLA, B.M.A. Possible role for sperm RNA in early embryo development.Theriogenology, v.68, n.1, p.147–155,2007.
- BORTOLOZZO, F.P.; WENTZ, I.; BENNEMANN, P.E.; BERNADI, M.L.; WOLLMANN, E.B.; FERREIRA, F.M.; NETO, G.B. Inseminação artificial na suinocultura tecnificada. Suinocultura em ação: Copyright, Porto Alegre, Brasil, 2005. 85p.
- BUHR, M.M.; CURTIS, E.F.; KAKUDA, N.S. Composition and behavior of head membrane lipids of fresh and cryopreserved boar sperm. Cryobiology, v.31, p.224-238, 1994.
- CABALLERO, I.; VAZQUEZ,J.M.; GARCÍA, E.M.; PARRILLA,I.; ROCA,J.; CALVETE, J.J.; SANZ, L.; MARTÍNEZ, E.A. Major proteins of boar seminal plasma as a tool for biotechnological preservation of spermatozoa. Theriogenology, v.70, n.8, p.1352–1355, 2008.
- CALVETE, J.J.; REINERT, M.; SANZ, L.; TÖPFER-PETERSEN, E. Effect of glycosylation on the heparin-binding capability of boar and stallion seminal plasma proteins. Journal of Chromatography, v.711, n.1, p.167–173, 1995.
- CALVETE, J.J.; SOLIS, D.; SANZ, L.; DIAZ-MURILLO, T.; SCHAFER, W.; MANN, K.; TÖPFER-PETERSEN, E. Characterization of two glycosylated boar spermadhesins. European Journal of Biochemistry, v.218, p.719-725, 1993.
- CASAS, I.; FLORES, E. Gene banking: the freezing strategy. In: BONET, S.; CASAS, I.; HOLT, W.V.; YESTE, M.Editors. Boar Reproduction. Berlin: Springer, p.551–88, 2013.
- CASAS, I.; SANCHO, S.; BRIZ, M.; PINART, E.; BUSSALLEU, E.; YESTE, M. Freezability prediction of boar ejaculates assessed by functional sperm parameters and sperm proteins. Theriogenology, v.72, n.7, p.930–948, 2009.
- CENTURIÓN, F.; VÁZQUEZ, J. M.; CALVETE, J. J.; ROCA, J.; SANZ, L.; PARRILLA, I.; GARCÍA, E. M.; MARTÍNEZ, E.A. Influence of porcine spermadhesins on the susceptibility of boar spermatozoa to high dilution. Biology of Reproduction, v.69, n.3, p.640–646, 2003.
- CHUAYCHU-NOO, N.; THANANURAK, P.; CHANKITISAKUL, V.; VONGPRALU, T. Supplementing rooster sperm with Cholesterol-Loaded-Cyclodextrin improves fertility after cryopreservation. Cryobiology, v.74, p.8-12, 2017.
- COLLARES, T.; BONGALHARDO, D.C.;DESCHAMPS, J.C.; MOREIRA, H.L.M. Transgenic animals: The melding of molecular biology and animal reproduction. Animal Reproduction, v.2, n.1, p.11-27, 2005.
- CORCINI, C.D.; VARELA, A.S.; PIGOZZO, R.; RAMBO, G.; GOULARTE, K.L.; CALDERAM, K.; LEON, P.M.M.; BONGALHARDO, D.C.; LUCIA JR., T. Pre-freezing and post-thawing quality of boar sperm for distinct portions of the ejaculate and as a function of protein bands presente in seminal plasma. Livestock Science, v.145, n.1, p.28-33, 2012.
- DE LEEUW, F.; COLENBRANDER, B.; VERKLEIJ, A. The role membrane damage plays in cold shock and freezing injury. Reproduction in Domestic Animals, v.1, n.1, p.95–104, 1990.
- DE JONGE, C.; BARRATT, C.L. Gamete donation: a question of anonymity. Fertility and Sterility, v.85, n.2, p.500-501, 2006.
- DU, J.; SHEN, J.; WANG, Y.; PAN, C.; PANG, W.; DIAO, H.; DONG, W. Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane. Oncotarget, v.7, n.37, p.58832–58847, 2016.
- EDDY, E. M.; O’BRIEN, D. A. The spermatozoon. In: KNOBIL, E.; NEILL, J. D. The Physiology of Reproduction. 2a ed., New York: Raven Press. Cap. 2, p.29-77, 1994.
- EKHALASI-HUNDRIESER, M.; SCHAFER, B.; KIRCHHOFF, C.; HESS, O.; BELLAIR, S.; MÜLLER, P.; TÖPFER- PETERSEN, E. Structural and molecular characterization of equine sperm-binding fibronectin-II module proteins. Molecular Reproduction Development, v.70, n.1, p.45–57, 2005.
- EVANS, G., MAXWELL, W.M C. Salamoninseminación artificial de ovejas y cabras. 1a ed., Zaragoza: Acribia, 1990. 191p.
- FLESCH, F.M.; GADELLA, B.M. Dynamics of the mammalian sperm plasma membrane in the process of fertilization.BiochimicaetBiophysica Acta, v.1469, n.3, p.197-235, 2000.
- FOXCROFT, G.R.; DYCK, M.K.; RUIZ-SANCHEZ, A.; NOVAK, S.; DIXON, W.T. Identifying useable semen. Theriogenology, v.70, n.8, p.1324–36,2008.
- GADELLA, B.M.; COLENBRANDER, B. Bicarbonate dependent capacitation of mammalian sperm cells: a comparative overview. In: Proceedings of a workshop on transporting gametes and embryos, 12, Brewster – Massachusetts, p.43-48, 2003.
- GAO, D.; CRITSER, J.K. Mechanisms of cryoinjury in living cells. ILAR Journal, v.41, n.4, p.187–196, 2000.
- GARCIA, E.M.; VÁZQUEZ, J.M.; CALVETE, J.J.; SANZ, L.; CABALLERO, I.; PARRILLA, I.; GIL, M.A.; ROCA, J.; MARTINEZ, E.A. Dissecting the protective effect of the seminal plasma spermadhesin PSP-I/PSP-II on boar sperm functionality. Journal of Andrology, v.27, n.3, p.434-443, 2006.
- GÓMEZ-FERNÁNDEZ, J.; GÓMEZ-IZQUIERDO, E.; TOMÁS, C.; MOCÉ, E.; DE MERCADO, E. Is sperm freezability related to the post-thaw lipid peroxidation and the formation of reactive oxygen species in boars? Reproduction in Domestic Animals, v.48, n.2m p.177–82, 2013.
- GRAHAM, J.K. Cryopreservation of stallion spermatozoa. Veterinary Clinics of North America: Equine Practice, v.12, n.1, p.131-147, 1996.
- GUIMARÃES, D.B.; BARROS, T.B.; VAN TILBURG, M.F.; MARTINS, J.A.M.; MOURA, A.A.; MORENO, F.B.; MONTEIRO-MOREIRA, A.C.; MOREIRA, R.A.; TONIOLLI, R. Sperm membrane proteins associated with the boar semen cryopreservation. Animal Reproduction Science, v.183, p.27-38, 2017.
- HAFEZ, E.S.E.; HAFEZ, B. Reprodução Animal. 7ª ed., Manole: São Paulo, 2003. 530p.
- HAFEZ, E.S.E. Preservação e criopreservação de gametas e embriões. In: __. Reprodução Animal. 6a ed.,Manole: São Paulo. Cap. 24, p.513-535, 1995.
- HAMMERSTEDT, R.H.; GRAHAM, J.K.; NOLAN, J.P. Cryopreservation of mammalian sperm; what we ask them to survive. Journal of Andrology, v.11, n.1, p.73-88, 1990.
- HOLT, W.V.; MEDRANO, A.; THURSTON, L.M.; WATSON, P.F. The significance of cooling rates and animal variability for boar sperm cryopreservation: insights from the cryomicroscope. Theriogenology, v.63, n.2, p.370–382, 2005.
- HOLT, W.V.; NORTH, R.D. Determination of liquid components and thermal phase transition temperature in an enriched plasma membrane fraction from spermatozoa. Journal of Reproduction and Fertility, v.73, p.285-294, 1985.
- JAISWAL, B.S.; CONTI, M. Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase. Journal of Biology and Chemestry, v.276, n.35, p.31.698–31.708, 2001.
- JOHNSON, L.A.; WEITZE, K.F.; FISER, P.; MAXWELL, W.M. Storage of boar semen. Animal Reproduction Science, v.62, n.1-3, p.143-172, 2000.
- KANG, S.; PANG, W-K.; RYU, D.-Y.; SONG, W.-H.; RAHMAN, M.S.; PARK, Y.-J.;PANG, M.-G. Porcine seminal protein-I and II mRNA expression in boar spermatozoa is significantly correlated with fertility. Theriogenology, v.138, n.1, p.31-38, 2019.
- KASIMANICKAM, R.; KASIMANICKAM, V.; THATCHER, C.D.; NEBEL, R.L; CASSELL B.G. Relationships among lipid peroxidation, glutathione peroxidase, superoxide dismutase, sperm parameters, and competitive index in dairy bulls. Theriogenology, v.67, n.7, p.1004–1012, 2007.
- KOPEIKA, J.; THORNHILL, A.; KHALAF, Y. The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence. Humain Reproduction Update, v.30, n.1, p.209–227, 2015.
- KOTHARI, S.; THOMPSON, A.; AGARWAL, A.; DU PLESSIS, S.S. Free radicals: their beneficial and de trimental effects on sperm function. Indian Journalof Experimental Biology, v.48, p.425-435, 2010.
- LEE, Y.S.; LEE, S.; LEE, S.H.; YANG, B.K.; PARK, C.K. Effect of cholesterol-loaded-cyclodextrin on sperm viability and acrosome reaction in boar semen cryopreservation. Animal Reproduction Science, v.159, p.124-130, 2015.
- LEHNINGER, A.L.; NELSON, D.L.; COX, M.M. Lehninger: Princípios de Bioquímica. 4ª ed. São Paulo: Editora Sarvier, 2005. 1120p.
- LI, J.; ROCA, J.; PÉREZ-PATIÑO, C.; BARRANCO, I.; MARTINEZ, E.A.; RODRIGUEZ-MARTINEZ, H.; PARRILLA, I. Is boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma? Animal Reproduction Science, v.195, p.30-37, 2018.
- LONE, S.A. Possible mechanisms of cholesterol-loaded cyclodextrin action on sperm during cryopreservation. Animal Reproduction Science, v.192, p.1-5, 2018.
- MACK, S.R.; EVERINGHAM, J.; ZANEVELD, L.J.D. Isolation and partial characterization of the plasma membrane from human spermatozoa. Journal of Experimental Zoology, v.240, n.1, p.125-136, 1986.
- MEDEIROS, C.M.O.; FORELL, F.; OLIVEIRA, A.T.D.; RODRIGUES, J.L. Current status of sperm cryopreservation: why isn't it better? Theriogenology, v.57, n.1, p.327-344, 2002.
- MEDRANO, A.; HOLT, W.V.; WATSON, P.F. Controlled freezing studies on boar sperm cryopreservation. Journal of Andrology, v.41, n.4, p.246–250, 2009.
- MOCÉ, E.; GRAHAM, J.K. Cholesterol-loaded cyclodextrins added to fresh bull ejaculates improve sperm cryosurvival. Journal of Animal Science, v.84, n.5, p.826–833, 2006.
- MOURA, A.A.; CHAPMAN, D.A.; KOC, H.; KILLIAN, G.J. A comprehensive proteomic analysis of the accessory sex gland fluid from mature Holstein bulls. Animal Reproduction Science, v.98, n.3-4, p.169-188, 2007.
- MORAN, D.M.; JASKO, D.J.; SQUIRES, E.L.; AMANN, R.P. Determination of temperature and cooling rate which induce cold shock in stallion spermatozoa. Theriogenology, v.38, p.999-1012, 1992.
- MUKAI, C.; OKUNO, M. Glycolysis plays a major role for adenosine triphosphate supplementation in mouse sperm flagellar movement. Biology of Reproduction, v.71, p.540–547, 2004.
- NEILLL, D.J. Physiology of Reproduction. 3a ed., vol. 1, 2006. 3296p.
- NESCI, S.; SPINACI, M.; GALEATI, G.; NEROZZI, C.; PAGLIARANI, A.; ALGIERI, C.; TAMANINI, C.; BUCCI, D. Sperm function and mitochondrial activity: An insight on boar sperm metabolism. Theriogenology, v.144, p.82-88, 2020.
- NOVAK, S.; RUIZ-SÁNCHEZ, A.; DIXON, W.T.; FOXCROFT, G.R.; DYCK, M.K. Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars. Journal of Andrology, v.31, n.2, p.188–200, 2010.
- PARKS, J.E.; GRAHAM, J.K. Effects of cryopreservation procedures on sperm membranes. Theriogenology, v.38, p.209-222, 1992.
- PAOLI, D.; LOMBARDO, F.; LENZI, A.; GANDINI, L. Sperm cryopreservation: effects on chromatin structure. Advances in Experimental Medicine and Biology, v.791, p.137–150, 2014.
- PEÑA, F.J.; DAVILA, M. P.; BALL, B.A.; SQUIRES, E.L.; MARTIN MUÑOZ, P, FERRUSOLA, C.O.; SILVA, C.B.The impact of reproductive technologies on stallion mitochondrial function. Reproduction in Domestic Animals, v.50, p.529–37, 2015.
- PEÑA, F.J.; SARAVIA, F.; NÚÑEZ-MARTÍNEZ, I.; JOHANNISSON, A.; WALLGREN, M.; RODRÍGUEZ MARTÍNEZ, H. Different portions of the boar ejaculate vary in their ability to sustain cryopreservation? Animal Reproduction Science, v.93, p.101–113, 2006.
- PEÑA, F.J.; JOHANNISSON, A.; WALLGREN, M. Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. Animal Reproduction Science, v.78, n.1-2, p.85-98, 2003.
- PRIETO‐MARTÍNEZ, N.; VILAGRAN, I.; MORATÓ, R.; ÁLAMO, M.M.R.; RODRÍGUEZ‐GIL, J.E.; BONET, S.; YESTE, .M. Relationship of aquaporins 3 (AQP3), 7 (AQP7), and 11 (AQP11) with boar sperm resilience to withstand freeze–thawing procedures. Andrology, v.5, n.6, p.1153-1164, 2017.
- RATH, D.; BATHGATE, R.; RODRÍGUEZ-MARTÍNEZ, H.; ROCA, J.; STRZEZEK, J.; WABERSKI, D. Recent advances in boar semen cryopreservation Society of Reproduction and Fertility, v.66, Suppl. 1, p.51–66, 2009.
- RECUERO, S.; FERNANDEZ-FUERTES, B.; BONET, S.; BARRANCO, I.; YESTE, M. Potential of seminal plasma to improve the fertility of frozen-thawed boar spermatozoa. Theriogenology, v.137, p.36-42, 2019.
- ROCA, J.; HERNANDEZ, M.; CARVAJAL, G.; VAZQUEZ, J.M.; MARTINEZ, E.A. Factors influencing boar sperm cryosurvival. Journal of Animal Science, v.84, n.10, p.2692–2699, 2006.
- RODRÍGUEZ-MARTÍNEZ, H.; SARAVIA, F.; WALLGREN, M.; TIENTHAI, P.; JOHANNISSON, A.; VÁZQUEZ, J.M.; MARTÍNEZ, E.; ROCA, J.; SANZ, L.; CALVETE, J.J. Boar spermatozoa in the oviduct. Theriogenology, v.63, p.514-535, 2005.
- RODRIGUEZ-MARTINEZ, H.; IBORRA, A.; MARTINEZ, P.; CALVETE, J.J. Immunoelectron microscopic imaging of spermadhesin AWN epitopes on boar spermatozoa bound in vivo to the zona pellucida. Reproduction Fertility Development, v.10, p.491–497, 1998.
- ROZEBOOM, K.J.; TROEDSSON, M.H.T.; HODSON, H.H.; SHURSON, G.C.; CRABO, B.G. The importance of seminal plasma on the fertility of subsequent artificial insemination in swine. Journal of Animal Science, v.78, n.2, p.443–448, 2000.
- SALAMON, S.; MAXWELL, W.M.C. Storage of ram semen. Animal Reproduction Science, v.62, n.1-3, p.77-111, 2000.
- SALMON, V.M.; CASTONGUAY, F.; DEMERS-CARON, V.; LECLERC, P.; BAILEY, J.L. Cholesterol-loaded cyclodextrin improves ram sperm cryoresistance in skim milk-extender. Animal Reproduction Science, v.177, n.1, p.1-11, 2017.
- SILVA, E.F.; CARDOSO, T.F.; TAVARES, G.C.; COSTA, V.G.G.; SILVA, J.F.; VARELA, A.S.JR.; LEITE, F.P.L.; CORCINI, C.D. Criopreservação de sêmen suíno e a pesquisa com antioxidantes. Revista Portuguesa de CiênciasVeterinárias, v.108, p.103-112, 2013.
- STRZEŻEK, J.; WYSOCKI, P.; KORDAN, W.; KUKLIŃSKA, M.; MOGIELNICKA, M.; SOLIWODA, D.; FRASER, L. Proteomics of boar seminal plasma – current studies and possibility of their application in biotechnology of animal reproduction. Reproduction and Biology, v.5, p.279-290, 2005.
- STRZEZEK, J. Secretory Activity of Boar Seminal Vesicle Glands. Reproductive Biology, v.2, n.3, p.243–66, 2002.
- THURSTON, L.M.; SIGGINS, K.; MILEHAM, A.J.; WATSON, P.F.; HOLT, W.V. Semen cryopreservation: a genetic explanation for species and individual variation? Cryo Letters, v.23, n.4, p.255–262, 2002.
- TONIOLLI, R.; GUIMARÃES, D.B.; BARROS, T.B. Proteínas do sêmen e sua relação com a resistência à congelação em ejaculados de diferentes varrões. Revista Brasileira de Reprodução Animal, v.41, n.1, p.297-311, 2017.
- TROEDSSON, M.H.; DESVOUSGES, A.; ALGHAMDI, A.S.; DAHMS, B.; DOW, C.A.; HAYNA, J.; VALESCO, R.; COLLAHAN, P.T.; MACPHERSON, M.L.; POZOR, M.; BUHI, W.C. Components in seminal plasma regulating sperm transport and elimination. Animal Reproduction Science, v.89, n.1-4, p.171–86, 2005.
- TURNER, R.M. Tales from the tail: what do we really know about sperm motility? Journal of Andrology, v.24, n.6, p.790-802, 2003.
- VADNAIS, M.L.; ALTHOUSE, G.C. Characterization of capacitation, cryoinjury, and the role of seminal plasma in porcine sperm. Theriogenology, v.76, p.1508–16, 2011.
- VALENCIA, J.; GÓMEZ, G.; LÓPEZ, W.; MESA, H.; HENAO, F.J. Relationship between HSP90a, NPC2 and L-PGDS proteins to boar semen freezability. Journal of Animal Science and Biotechnology, v.8, p.1-10, article 21, 2017.
- VILAGRAN, I.; YESTE, M.; SANCHO, S.; CASAS, I.;RIVERA DEL ALAMO, M.M.; BONET, S. Relationship of sperm small heat-shock protein 10 (ODF1/HSPB10) and voltage-dependent anion channel 2 (VDAC2) with semen freezability in boars. Theriogenology, v.82, p.418–426, 2014.
- WATERHOUSE, K.E.; HOFMO, P.O.; TVERDAL, A.; MILLER JR, R.R. Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm. Reproduction, v.131, n.5, p.887–894, 2006.
- WATSON, P.F. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reproduction Fertility and Development, v.7, n.4, p.871–891, 1995.
- YESTE, M. State-of-the-art of boar sperm preservation in liquid and frozen state. Animal Reproduction, v.14, n.1, p.69-81, 2017.