Effects of medium supplementation with different antioxidants during in vitro maturation of bovine oocytes on oxidative status
Keywords:
In vitro embryo production, Oxidative homeostasis, CattleAbstract
Although widely used as reproductive biotechnology in cattle, in vitro embryo production (IVEP) has variable efficiency. During in vitro maturation (IVM), supplementing the medium with antioxidant potential could be an affordable alternative to increase the efficiency of IVEP, requiring the evaluation of new components, such as eugenol, β-caryophyllene, and acetyl eugenol. Thus, bovine oocytes were matured according to different antioxidants. Metaphase II oocytes were identified by Hoechst staining. The oxidative status was measured by evaluation of reactive oxygen species (ROS), and glutathione (GSH). After eight repetitions, no difference was observed among groups containing antioxidants, being all these groups superior to negative control (p<0.05). The ROS levels decreased and GSH increased in oocytes matured with EUG (p<0.05). These results indicate a positive effect of eugenol on the oxidative status of oocytes matured with this compound, showing that eugenol can be an efficient supplement in the IVM of bovine oocytes.
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