Biotechnological potential of sex-sorting sperm in animal production
Keywords:
Flow cytometry, density gradients, injuries, Percoll, sperm populationsAbstract
The predetermination of progeny sex is a desire among breeders, since it allows the sexing of born animals according the purpose of the production system. With the discovery of the sex chromosomes X and Y, the sex pre-selection studies followed a scientific direction, in order to develop techniques for the separation of sperm populations with these chromosomes. For this purpose it was developed and improved the flow cytometer separation method, which is highly accurate, although presenting a high cost and causing sperm injuries. Therefore, alternative protocols have been sought to enable sperm sexing, without major structural and functional damage to gametes, with emphasis on Percoll® density gradients. Due to the biotechnological potential of sperm sexing, in order to maximize the animal production, it was objectified in this review expose the positive and negative points of the two main techniques used for this purpose.
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