TIME-COURSE RESPONSE OF CATALASE ENZYME IN SKELETAL MUSCLE OF RATS AFTER EXHAUSTIVE EXERCISE SESSION
Keywords:
Exhaustive Exercise, Catalase, Skeletal muscleAbstract
Studies show that exhaustive exercise prints in an imbalance between free radicais and antioxidant defense system. One of the enzymes responsible for the defense system is catalase (CAT) which detoxifica hydrogen peroxide in skeletal muscle avoiding possible damage. The aim of this study was to assess the time course of response catalase in skeletal muscle of mice after an exhaustive exercise session. They used 36 male Wistar rats with 60 days old and weigh ing 220-240g , kept in light / dark 12h / 12h with water and food ad libitum. The animais were divided into six groups: contrai group; immediately after exercise (Oh); 6h; 12h; 24h; 48 hours after exercise. These have been adapted for two weeks on a treadmill for 48 hours after the animais and adaptation undergo a workout. The portions of red gastrocnemius muscle were dissected and stored in liquid nitrogen. The gene expression analysis was performed by PCR in real time. To analyze the enzymatic activity of CAT, the cultures were placed in a spectrophotometer at the absorbance value at 240 nm. The results were evaluated by analysis of variance (ANOVA - oneway) and post Tukey test (p <0.05). The results showed large capacity regulation of this antioxidant enzyme , indicating a significant increase in expression of CAT 6h after exercise (p <0.05), as well as enzyme activity also increased by 6 hours after exercise (p <0.05) compareci with the contrai, concluding that in the first hour after exercise has been possible to identify a defense response in skeletal muscle after exhaustive exercise.
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