Molecular detection of Shiga Toxin-producingEscherichia coli in foods
Keywords:
Escherichia coli, Shiga Toxin, Molecular assays, PCR, STECAbstract
Culture-independent methods are proposed as a tool for detecting pathogenic microorganisms in food. Escherichia coli strains that produce Shiga Toxin lead to diseases such as bloody diarrhea and hemolytic uremic syndrome by causing damage to the intestine and releasing the toxin into the body. Serological differentiation methods investigate the cell wall antigens, characteristic of each group of E. coli, however they are not efficient when it comes to a more accurate investigation of associated virulence factors. Thus, molecular methods are based on the search for specific genes related to the mechanisms of invasion and damage to the epithelium, as they are protein complexes. The present study was designed with the objective of carrying out a brief literature review about molecular methods as a detection tool for Shiga toxin-producing Escherichia coli. The methodology used dealt with a systematic review in the following databases Web of Science, Science Direct and PUBMED.
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