DETECTION OF Leishmania chagasi IN CATS (Felis catus) FROM VISCERA LEISHMANIASIS ENDEMIC AREA IN BRAZIL
Parole chiave:
Leishmaniasis, PCR-RFLP, catAbstract
The number of confirmed cases of feline leishmaniasis has increased in endemic areas in recent years, which suggests that the feline species should no longer be considered an uncommon host for Leishmaniasis spp. Thus, the objective of this work was to investigate the occurrence of Leishmaniasis spp infection in cats in the city of Campo Grande, where visceral leishmaniasis is endemic. The 110 cats studied were subjected to clinical and serological examination by IFA to determine the titer of anti-Leshimania antibodies. The parasitological investigation of seropositive animals consisted of cytological examination, tissue culture, and PCR of bone marrow aspirates and popliteal lymph nodes. Serum positivity was detected in eight animals (7.27%). Amastigotes were not detected by cytological examinations and promastigotes were not observed in tissue culture. However, three (40.2%) of the seven processed samples were positive according to PCR, which confirms the cat's contact with the parasite.
Riferimenti bibliografici
ANTUNES, T. R. et al. Técnicas de citologia aspirativa, biópsia e citobloco de medula óssea para identificação e determinação de intensidade parasitária na leishmaniose visceral canina. Arquivos Brasileiros de Medicina Veterinária e Zootecnia, v.70, n.5, p.1362-1368, 2018.
BASSO, M. A. et al. Successful treatment of feline leishmaniosis using a combination of allopurinol and N-methyl-glucamine antimoniate. Journal of Feline Medicine and Surgery Open Reports, v.2, n.1, p. 1-7, 2016.
BENASSI, J. C. et al. Molecular and serological detection of Leishmania spp. in horses from an endemic area for canine visceral leishmaniasis in southeastern Brazil. Pesquisa Veterinária Brasileira, v.18, n.38, p.1058–1063, 2018.
CARNEIRO, E.C. Protozoários Flagelados. In: MONTEIRO, S.G. Parasitologia na Medicina Veterinária. Rio de Janeiro: Editora Roca, 2018. p. 133-141.
ESCOBAR, T. A. et al. Assessment of Leishmania infantum infection in equine populations in a canine visceral leishmaniosis transmission area. BMC Veterinary Research, v.15, n.381, p.1-9, 2019.
GONTIJO, B. B. et al. Esporotricose e Leishmaniose Tegumentar em cães e gatos: semelhanças e diferenças. Pubvet, v.5, n.38, p. 1245- 1250, 2011.
LANGONI, H. Leishmanioses. In: GREENE, C.E. Doenças infecciosas de cães e gatos. Rio de Janeiro: Editora Roca, 2016. p. 1013-1024.
BEZERRA, J. A. B. et al. Investigação sorológica e molecular da infecção por Leishmania spp. em gatos provenientes de uma área endêmica para leishmaniose canina e humana no nordeste brasileiro. Revista Brasileira de Parasitologia Veterinária, v. 28, p. 790-796, 2019.
BRIANTI, E. et al. Prevention of feline leishmaniosis with an imidacloprid 10% flumethrin 4.5% polymer matrix collar. Parasites Vectors, v.10, n.334, p.1-8, 2017.
