Exogenous protease: effects on intestinal health and performance of poultry and weaned piglets
Keywords:
Poultry farming, Nutrient digestibility, Microbiota, Nutrition, Pig farmingAbstract
The use of exogenous enzymes is one of the feeding strategies used in the nutrition of non-ruminant animals. The exogenous protease is essential to ensure higher and better use of plant protein sources used in diets for poultry and swine. Associated with this, the lower presence of nitrogen in the excreta and feces of the animals should also be considered, due to the increase in the efficiency of the digestion process and absorption of protein nutrients. Moreover, the maintenance of intestinal health has become an essential strategy to ensure better animal performance. In this context, the better use of proteins in the small intestine followed by the reduction of fermentable protein in the large intestine is considered a beneficial effect promoted by the action of protease, as it reduces the substrate content for pathogenic bacteria action, ensuring a healthy and efficient microbiota. Given the above, this study aimed to present the main exogenous protease actions used in the nutrition of poultry and weaned piglets, as well as its effects on intestinal health and animal performance.
References
ALAGAWANY, M.; ELNESR, S.S.; FARAG, M.R. The role of exogenous enzymes in promoting growth and improving nutrient digestibility in poultry. Iranian Journal of Veterinary Research, v.19, n.3, p.157–164, 2018.
AMIRI, M.Y.A.; JAFARI, M.A.; IRANI, M. Growth performance, internal organ traits, intestinal morphology, and microbial population of broiler chickens fed quinoa seed–based diets with phytase or protease supplements and their combination. Tropical Animal Health and Production. v.53, n.6, p.535, 2021.
BAREKATAIN, R.; NATTRASS, G.G.; TILBROOK, A.J.; CHOUSALKAR, K.; GILANI, S. Reduced protein diet and amino acid concentration alter intestinal barrier function and performance of broiler chickens with or without synthetic glucocorticoid. Poultry Science. v.98, n.9, p.3662–3675, 2019.
BEDFORD, M.R.; APAJALAHTI, J.H. The role of feed enzymes in maintaining poultry intestinal health. Journal of the Science of Food and Agriculture, v.102, n.5, p.1759-1770, 2022.
BURDICK SANCHEZ, N.C.; BROADWAY P.R.; CARROLL, J.A. Pre-and probiotic effects on innate immunity and metabolism in cattle and swine. In: KOGUT, M.H., ZHANG, G. (eds) Gut Microbiota, Immunity, and Health in Production Animals. The Microbiomes of Humans, Animals, Plants, and the Environment. Springer, Cham, v.4, p.277-297, 2022.
BORDA-MOLINA, D.; ZUBER, T.; SIEGERT, W.; CAMARINHA-SILVA, A.; FEUERSTEIN, D.; RODEHUTSCORD, M. Effects of protease and phytase supplements on small intestinal microbiota and amino acid digestibility in broiler chickens. Poultry Science. v.98, n.7, p.2906–2918, 2019.
CARDINAL, K.M.; MORAES, M.L.; ANDRETTA, I.; SCHIRMANN, G.D.; BELOTE, B.L.; BARRIOS, M.A.; SANTIN, E.; RIBEIRO, A.M.L. Growth performance and intestinal health of broilers fed a standard or low-protein diet with the addition of a protease. Revista Brasileira de Zootecnia. v.48, n.10, p.1-11, 2019.
COWIESON, A.J.; ABDOLLAHI, M.R.F.; ZAEFARIAN, F.; PAPPENBERGER, G.; RAVINDRAN, V. The effect of a mono-component exogenous protease and graded concentrations of ascorbic acid on the performance, nutrient digestibility, and intestinal architecture of broiler chickens. Animal Feed Science and Technology. v.235, n.1, p.128–137, 2018.
DAL PONT, G.C.; EYNG, C.; BORTOLUZZI, C.; KOGUT, M.H. Enzymes and gut health in monogastric animals: effects beyond digestibility. In: KOGUT, M.H., ZHANG, G. (eds) Gut Microbiota, Immunity, and Health in Production Animals. The Microbiomes of Humans, Animals, Plants, and the Environment, Springer, Cham, v.4, p.33–55, 2022.
DUARTE, M.E.; ZHOU, F.X.; DUTRA JR, W.M.; KIM, S.W. Dietary supplementation of xylanase and protease on growth performance, digesta viscosity, nutrient digestibility, immune and oxidative stress status, and gut health of newly weaned pigs. Animal Nutrition. v.5, n.4, p.351-358, 2019.
KIM, Y.J.; LEE, J.H.; KIM, T.H.; SONG, M.H.; YUN, W.; OH, H.J.; LEE, J.S.; KIM, H.B.; CHO, J.H. Effect of low protein diets added with protease on growth performance, nutrient digestibility of weaned piglets and growing finishing pigs. Journal of Animal Science and Technology, v.63, n.3, p.491–500, 2021.
KNECHT, D.; CHOLEWINSKA, P.; MAKOSA, A.J.; CZYZ, K. Development of swine’s digestive tract microbiota and its relation to production indices - A Review. Animal. v.10, n.3, p.1-13, 2020.
LEE, J.J.; KANG, J.; PARK, S.; CHO, J.H.; OH, S.; PARK, D-J.; PEREZ-MALDONADO, R.; CHO, J.Y.; PARK, PARK, I-H.; KIM, H.B.; SONG, M. Effects of dietary protease on immune responses of weaned pigs. Journal of Animal Science and Technology, v.62, n.2, p.174-179, 2020.
LEI, X.J.; CHEONG, J.Y.; PARK, J.H.; KIM, I.H. Supplementation of protease, alone and in combination with frutooligosaccharide to low protein diet for finishing pigs. Journal of Animal Science, v.88, n.2, p.1987-1993, 2017.
LI, Y.; LEI, X.; GUO, W.; WU, S.; DUAN, Y.; YANG, X.; YANG, X. Transgenerational ET tolerance-like effect caused by paternal dietary astragalus polysaccharides in broilers’ jejunum. International Journal of Biological Macromolecules, v.111, n.5 p.769-779, 2018.
LI, Z.; TANG, L.; LIU, N.; ZHANG, F.; LIU, X.; JIANG, Q.; CHEN, J.; MA, X. Comparative Effects of Compound Enzyme and Antibiotics on Growth Performance, Nutrient Digestibility, Blood Biochemical Index, and Intestinal Health in Weaned Pigs. Frontiers in Microbiology, v.12, n.9, p.1-11, 2021.
LIMA, M.D.; LOPES, I.M.G.; SILVA, K.F.; MIRANDA, H.A.F.; ALMEIDA, A.C.; DUARTE, E.R. Uso de aditivos em dietas para leitões em fase de creche: uma revisão. Research, Society and Development, v.9, n.12, p.1-31, 2020.
LONG, S.; XU, J.; MA, H.; PIAO, X. Effects of dietary supplementation of compound enzymes on performance, nutrient digestibility, serum antioxidant status, immunoglobulins, intestinal morphology and microbiota community in weaned pigs. Archives of Animal Nutrition, v.75, n.1, p.31-47, 2020.
LOURENÇO, J.M.; NUNN, S.C.; LEE, E.J.; ROBERT DOVE, C.; CALLAWAAY, T.R.; AZAIN, M.J. Effect of supplemental protease on growth performance and excreta microbiome of broiler chicks. Microorganisms, v.8, n.4, p.1-15, 2020.
MAPA. Ministério da Agricultura, Pecuária e Abastecimento. Aditivo para produtos destinados à alimentação animal. Instrução Normativa n° 13 de 30, de novembro de 2004. Disponível em: , Acesso em: 7 set 2022.
MAQSOOD, M.A.; KHAN, E.U.; QAISRANI, S.N.; RASHID, M.A.; SHAHEEN, M.S.; NAZIR, A.; TALIB, H.; AHMAD, S. Interactive effect of amino acids balanced at ideal lysine ratio and exogenous protease supplemented to low CP diet on growth performance, carcass traits, gut morphology, and serum metabolites in broiler chicken. Tropical Animal Health and Production, v.54, n.3, p.186, 2022.
McCAFFERTY, K.W.; CHOCT, M.; MUSIGWA, S.; MORGAN, N.K.; COWIESON A.J.; MOSS, A.F. Protease supplementation reduced the heat increment of feed and improved energy and nitrogen partitioning in broilers fed maize-based diets with supplemental phytase and xylanase. Animal Nutrition, v.10, n.9, p.19-25, 2022.
METZLER-ZEBELI, B.U. Porcine gut microbiota and host interactions during the transition from the suckling to postweaning phase. In: KOGUT, M.H., ZHANG, G. (eds) Gut Microbiota, Immunity, and Health in Production Animals. The Microbiomes of Humans, Animals, Plants, and the Environment, Springer, Cham, v.4, p.147-178, 2022.
MIN, Y.; CHOI, Y.; CHOE, J.; KIM, J.; JEONG, Y.; KIM, D.; KIM, J.; JUNG, H.; SONG, M. Effects of dietary mixture of protease and probiotics on growth performance, blood constituents, and carcass characteristics of growing-finishing pigs. Journal of Animal Science and Technology, v.61, n.5, p.272-277, 2019.
MOESER, A.J.; POHL, C.S.; RAJPUT, M. Weaning stress and gastrointestinal barrier development: Implications for lifelong gut health in pigs. Animal Nutrition. v.3, n.4, p.313-321, 2017.
NDAZIGARUYE, G.; KIM, D.H.; KANG, C.W.; KANG, K.R.; JOO, Y.J.; LEE, S.R.; LEE, K.W. Effects of Low-Protein Diets and Exogenous Protease on Growth Performance, Carcass Traits, Intestinal Morphology, Cecal Volatile Fatty Acids and Serum Parameters in Broilers. Animals, v.9, n.5, p.226, 2019.
PARK, S.; LEE, J.J.; YANG, B.M.; CHO, J.H.; KIM, S.; KANG, J.; OH, S.; PARK, D.J.; PEREZ-MALDONADO, R.; CHO, J.Y.; PARK, I-H.; KIM, H.B.; SONG, M. Dietary protease improves growth performance, nutrient digestibility, and intestinal morphology of weaned pigs. Journal of Animal Science and Technology, v.62, n.1, p.21-30, 2020a.
PARK, S.; LEE, J.W.; COWIESON, A.J.; PAPPENBERGER G.; WOYENGO, T.A. Soybean meal allergenic protein degradation and gut health of piglets fed protease-supplemented diets. Journal of Animal Science, v.98, n.10, p.1-35, 2020b.
PENG, X.; ZHOU, Q.; WU, C.; ZHAO, J.; TAN, Q.; HE, Y.; HU, L.; FANG, Z.; LIN, Y.; XU, S.; FENG, B.; LI, J.; ZHUO, Y.; VAN GINNEKEN, C.; JIANG, X.; WU, D.; CHE, L. Effects of dietary supplementation with essential oils and protease on growth performance, antioxidation, inflammation and intestinal function of weaned pigs. Animal Nutrition, v.9, n.7, p.39-48, 2022.
PEREZ-PALENCIA, J.Y.; SAMUEL, R.S.; LEVESQUE, C.L. Supplementation of protease to low amino acids diets containing superdose level of phytase for wean-to-finish pigs: effects on performance, postweaning intestinal health and carcass characteristics. Translational Animal Science, v.5, n.2, p.1-14, 2021.
ROSS, G.R.; GUSILS, C.; OLISZEWSKI, R.; HOLGADO, S.C.; GONZÁLEZ, S.N. Effects of probiotic administration in swine. Journal of Bioscience and Bioengineering, v.109, n.6, p.545-549, 2010.
SILVA, J.M.S.; OLIVEIRA, N.R.; GOUVEIA, A.B.V.; VIEIRA, S.R.A.; SANTOS, R.O.; MINAFRA, F.C.S.; SANTOS, F.R. Effect of protease supplementation on the digestibility of amino acids in animal-origen meals for broiler diets. Czech Journal of Animal Science, v.66, n.1, p-29-37,2021.
SONG, M.; KIM, B.; CHO, J.H.; KYOUNG, H.; PARK, S.; CHO, J.Y.; PARK, K.; KIM, H.B., LEE, J.J. Effects of dietary protease supplementation on growth rate, nutrient digestibility, and intestinal morphology of weaned pigs. Journal of Animal Science and Technology, v.64, n.3, p.462-470, 2022.
SOUZA, C.G.; MOURA, A.K.B.; SILVA, J.N.P.; SOARES, K.O.; SILVA, J.V.C.; VASCONCELOS, P.C. Fatores anti-nutricionais de importância na nutrição animal: Composição e função dos compostos secundários. Pubvet, v.13, n.5, p.1-19, 2019.
SOUZA, J.P.P.; LIMA, M.D.; LOPES, I.M.G. Enzimas exógenas na dieta de leitões desmamados. Ciência Animal, v.32, n.2, p.68-84, 2022.
TACTACAN, G.B.; CHO, S.Y.; CHO, J.H.; KIM, I.H. Performance responses, nutrient digestibility, blood characteristics, and measures of gastrointestinal health in weanling pigs fed protease enzymes. Asian-Australasian Journal of Animal Sciences, v.29, n.7, p.998-1003, 2016.
WALK, C.L.; JUNTUNEN, K.; PALOHEIMO, M.; LEDOUX, D.R. Evaluation of novel protease enzymes on growth performance and nutrient digestibility of poultry: Enzyme dose response. Poultry Science, v.98, n.11, p.5525–5532, 2019.
WANG, Q.D.; LI, S.; ZHANG, K.Y.; BAI, Y.; BAI, S.P.; DING, X.M.; WANG, J.P.; PENG, H.W.; TIAN, G.; XUAN, Y.; SU, Z.W.; ZENG, Q.F. Protease supplementation attenuates the intestinal health damage caused by low-protein diets in Pekin ducks. Poultry Science, v.99, n.12, p.6630-6642, 2020.
YAKOUT, H.M.; ECKHARDT, E. Gastrointestinal tract barrier efficiency: function and threats. In: KOGUT, M.H., ZHANG, G. (eds) Gut Microbiota, Immunity, and Health in Production Animals. The Microbiomes of Humans, Animals, Plants, and the Environment, 4, Springer, Cham, v.4, p.13-32, 2022.
YU, J.; YU, G.; YU, B.; ZHANG, Y.; HE, J.; ZHENG, P.; MAO, X.; LUO, J.; HUANG, Z.; LUO, Y.; YAN, H.; WANG, Q.; WANG, H.; CHEN, D. Dietary protease improves growth performance and nutrient digestibility in weaned piglets fed diets with different levels of soybean meal. Livestock Science, v.241, n.11, p.1-7 2020.
ZHANG, G.G.; YANG, Z.B.; WANG, Y.; YANG, R.W.; ZHOU, H.J. Effects of dietary supplementation of multi-enzyme on growth performance, nutrient digestibility, small intestinal digestive enzyme activities and large intestinal selected microbiota in weanling pigs. Journal of Animal Science, v.92, n.5, p.2063–2069, 2014.
ZHANG, L.; PIAO, X. Different dietary protein sources influence growth performance, antioxidant capacity, immunity, fecal microbiota, and metabolites in weaned piglets. Animal Nutrition, v.8, n.1, p.71-81, 2022.
ZUO, J.; LING, L.; LONG, T.; LI, L.; LAHAYE, C.; YANG, FENG, D. Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes, and gene expression of weaned piglets. Animal Nutrition, v.1, n.4, p.276-286, 2015.