Evaluation of the effects of pyometra on the viability of immature canine oocytes through the use of brilliant cresyl blue
Keywords:
Sanity, canine oocyte quality, supravital dye, bitchAbstract
The bitch is an experimental model of wild and even endangered canids. Therefore, the study of the factors that influence your fertility benefits scientific advances in both segments. Knowing that pyometra is one of the common uterine pathologies in bitches, this work aimed to evaluate the effects of pyometra on the morphology and competence of canine oocytes through the use of Azul CresilBrilhante (ACB). For this purpose, 1197 canine oocytes that were divided into 2 groups classified as control (healthy) and treatment (pyometra). They were morphologically classified into grade 1 (G1), grade 2 (G2) and grade 3 (G3) and according to the ACB stain as ACB (+) and ACB (–) (not stained). Bitches in the healthy group had higher amounts of total oocytes (795) and better quality (495 oocytes G1) and competence (45 ± 9.8 ACB (+)). The use of ACB was useful to distinguish the competence of the studied oocytes and can be an auxiliary tool for choosing the best oocytes.
References
ASA, C.S.; BAUMAN, K.L.; DEVERY, S.; ZORDAN, M.; CAMILO, G.R.; BOUTELLE, S.; MORESCO, A. Factors associated with uterine endometrial hyperplasia and pyometra in wild canids: implications for fertility. Zoo Biology,v.99, n.1, p.1-12, 2013.
CARREIRO, A.N.; SOUZA, J G.; LA SALLES, A.Y.F.; FALCÃO, B.M.R.; ARAÚJO, D.V.F.; DINIZ, J.A.R.A.; ROCHA, E.F.; ARAÚJO, N.L.S.; MENEZES, D.J.A. Obtenção de oócitos em cadelas e gatas submetidas à ovariosalpingohisterectomia. Pubvet, v.12, n.6, p.1-6. 2018.
HEWITT, D.A.; ENGLAND, G.C.W. The effect of pre-ovulatory endocrine events upon maturation of oocytes of the domestic bitch. Journal of Reproduction and Fertility, v.51, supl.51, p.83-91. 1997.
JEWGENOW, K.; FERNANDEZ-GONZALEZ, L.; JÄNSCH, S.; VIERTEL, D.; ZAHMEL, J. Brilliant cresyl blue staining allows the selection for developmentally competent immature feline oocytes. Theriogenology, v.126, n.1, p.320–325, 2019.
KARAMISHABANKAREH, H.; MIRSHAMSI, S.M. Selection of developmentally competent sheep zygotes using the Brilliant Cresyl Blue (ACB) test after IVF. Small Ruminant Research, v.105, n.1, p.250-254, 2012.
OPIELA, J.; KĄTSKA-KSIĄŻKIEWICZ, L. The utility of Brilliant Cresyl Blue (ACB) staining of mammalian oocytes used for in vitro embryo production (IVP). Reproductive Biology, v.13, n.3, p.177-183, 2013.
PEREIRA,L.M.C.;BICUDO,S.D.;LOPES,M.D. Oocyte maturation in bitches. Animal Reproduction, v.9, n.3, p.205-209. 2012.
