Different types of packaging and quality of cryopreserved swine semen
Keywords:
Freezing, sperm motility, thermoresistance, boarAbstract
Due to high sensitivity of the swine spermatozoa to cryopreservation, it is important to study the damage caused to the cell by thermal aggression during this process. Thus, this study evaluated the influence of different packages used for the storage of swine semen on the quality of cryopreserved spermatozoa. Hybrid animals were used, and semen collection was made by the gloved hand technique. The vigor (0 the 5), motility (0 the 100%), rate of motility degradation and acrosome integrity were analyzed. The semen was frozen in straws of 0.5mL, cryotubes of 2.0mL and macrotubes of 4 and 5mL. The swine semen samples frozen in straws showed the best results in sperm vigor (2.3), motility (45.4%), motility degradation rate (56.5%) and acrosomal integrity (60.6%), when compared to the cryopreserved samples in the other types of packaging evaluated (p<0.05). Only for the motility degradation rate parameter, the semen preserved in straws showed results similar to the one conserved in a 4 mL macrotube (54.1%). The results after thawing indicated that the semen packed in straws was the one that presented consistent characteristics with the possibility of being used in artificial insemination protocols with possibilities of good fertility results. It was concluded that packages that allow a higher speed of temperature changes between the cells found on the edge and in the center of them, favor a better quality of the thawed semen.
References
ABDEL-KHALEK, A.E.; KHALIL, W.A.; EL-SAIDY, B.E.; YOUSIF, A.I.A. Effect of Some Alternative Components of Egg Yolk in Tris-Extender on Sperm Characteristics of Ram Semen Frozen with Two Methods of Packaging Semen. Journal of Animal Poultry Productrion, Article 6, v.9, n.1, p.33-40, 2018.
BERNARDI, M.L.; BORTOLOZZO, F.P.; WENTZ, I. Emprego de sêmen congelado na IA de suíno. Suinocultura em ação. Inseminação Artificial em Suinocultura Tecnificada, p.159-179, 2005.
BERNECIC, N.C.; ZHANG, M.; GADELLA, B.M.; BROUWERS, J.F.H.M.; JANSEN, J.W.A.; ARKESTEIJN, G.J.A.; GRAAF, S.P.; LEAHY, T. Bodipy-cholesterol can be reliably used to monitor cholesterol efflux from capacitating mammalian spermatozoa. Scientific Reports, v.9, article 9804, p.1-12, 2019.
BURANAAMNUAY, K.; TUMMARUK, P.; SINGLOR, J.; RODRIGUEZ-MARTINEZ, H.; TECHAKUMPHU, M. Effects of Straw Volume and Equex-STM® on Boar Sperm Quality after Cryopreservation. Reproduction in Domestic Animals, v.44, n.1, p.69-73, 2009.
BWANGA, C.O.; EKWALL, H.; RODRÍGUEZ-MARTÍNEZ, H. Cryopreservation of boar semen III. Ultrastructure of boar spermatozoa frozen ultra-rapidly at various stages of conventional freezing and thawing. Acta Veterinary Scandinavica, v.32, n.4, p.431-453, 1991.
BERNARDI, M.L.; BORTOLOZZO, F.P.; WENTZ, I. Emprego de sêmen congelado na IA de suíno. Suinocultura em ação. Inseminação Artificial em Suinocultura Tecnificada, p.159-179, 2005.
BORTOLOZZO, F.P.; WENTZ, I.; BENNEMANN, P.E.; BERNARDI, M.L.; WOLLMANN, E.B.; FERREIRA, F.M.; NETO, G.B. Suinocultura em ação: Inseminação artificial na suinocultura tecnificada. 1ª ed., Porto Alegre: Brasil; 2005. 185p.
CEROLINI, C.; MALDJIAN, A.; PIZZI, F.; GLIOZZI, T.M., Changes in sperm quality and lipid composition during cryopreservation of boar semen. Reproduction in Domestic Animals, v.121, n.2, p.395-401, 2001.
CBRA. Colégio Brasileiro de Reprodução animal. Manual para Exame Andrológico e Avaliação de Sêmen Animal. 3a ed., Belo Horizonte: CBRA, 2013. 104p.
CÓRDOVA, A.; PÉREZ-GUTIÉRREZ, J.F.; LLEÓ, B.; GARCÍA-ARTIGA, C.; ALVAREZ, A.; DROBCHAK, V.; MARTÍN-RILLO, S. In vitro fertilizing capacity and chromatin condensation of deep-frozen boar semen packaged in 0.5 and 5mL straws. Theriogenology, v.57, p.2119-2128, 2002.
COSTA BARROS, M.H.; SHIOMI, H.H.; AMO, L.S.; GUIMARÃES, S.E.F.; LOPES, P.S.; SIQUEIRA, J.B.; PINHO, R.O.; GUIMARÃES, J.D. Viabilidade espermática de sêmen congelado de suínos da raça Piau avaliada pelo teste de termorresistência. Revista Brasileira de Medicina Veterinária, v.36, n.2, p.131-136, 2014.
COURTENS, J.L.; PAQUIGNON, M. Ultrastructure of fresh, frozen and frozen thawed spermatozoa of the boar. In: Johnson, L.A. Larson, K. Deep freezing of boar semen. Swedish University Agriculture Science, Uppsala, p.61-87, 1985.
DAVOODIAN, N.; KADIVA, A.; AHMADI, E.; MOHEBBI, A. Effects of Two Amino Acids on Motion Parameters and Enzymatic Antioxidant Activity of Freeze-Thawed Stallion Spermatozoa. Journal of Equine Veterinary Science, v.59, p.49-56, 2017.
EKWALL, H. Cryo-scanning electron microscopy discloses differences in dehydration of frozen boar semen stored in large containers. Reproduction in Domesitc Animals, v.44, n.1, p.62–68, 2009.
ERIKSSON, B.M.; RODRÍGUEZ-MARTÍNEZ, H. Effect of freezing and thawing rates on the pos-thaw viability of boar spermatozoa frozen in flatpaks and maxi-straws. Animal Reproduction Science, v.63, n.3-4, p.205-220, 2000.
ERIKSSON, B.M.; VAZQUEZ, J.M.; MARTINEZ, E.A.; ROCA, J.; LUCAS, X.; RODRIGUEZMARTINEZ, H. Effects of holding time during cooling and of type of package on plasma membrane integrity, motility and in vitro oocyte penetration ability of frozen-thawed boar spermatozoa. Theriogenology, v.55, n.8, p.1593-1605, 2001.
ERIKSSON, B.M.; RODRÍGUEZ-MARTÍNEZ, H. Deep-freezing of boar semen in plastic film ‘Cochettes”. Journal of Veterinary Medicine, v.47, n.1, p.89-97, 2002.
FISER, P.S.; FAIRFULL, R.W. Combined effect of glycerol concentration and cooling velocity on motility and acrosomal integrity of boar spermatozoa frozen in 0.5 ml straws. Molecular Reproduction and Development, v.25, n.2, p.123-129, 1990.
GUIMARÃES, D.B.; BARROS, T.B.; CANTANHÊDE, L.F.; FEUGANG, J.M.N.; SOUZA, L.P.; TONIOLLI, R. Qualidade espermática durante a curva de resfriamento do sêmen suíno diluído em água de coco em pó visando sua criopreservação. Ciência Animal Brasileira, v.19, p.1-16, 2018.
GRAHAM, J.K.; MOCÉ, E. Fertility evaluation of frozen/thawed semen. Theriogenology, v.64, n.3, p.492-504, 2005.
IZQUIERDO, A.C.; JIMÉNEZ, M.S.C.; JIMÉNEZ, C.A.L.; GUTIERRÉZ, J.P.; RILLO, S.M. Congelación de semen de verraco en dos tipos de pajillas y capacidad fecundante in vitro de los espermatozoides. Ciencia Natural Agropecuária, v.12, p.271-274, 2005.
KHALIL, W.A.; ABDEL-KHALEK, E.; FALCHI, L.; EL-SAIDY, B.E.; YOUSIF, A. Effects of extender and packaging method on morphological and functional characteristics of cryopreserved Ossimi ram semen. Asian Pacific Journal of Reproduction, v.9, n.3, p.148-155, 2020.
LAZARI, F.L.; SONTAG, E.R.; SCHNEIDER, A.; MOURA, A.A.A.; VASCONCELOS, F.R.; NAGANO, C.S.; MATTOS, R.C.; JOBIM, M.I.M.; BUSTAMANTE-FILHO, I.C. Proteínas plasmáticas seminal e sua relação com a motilidade e morfologia do esperma em javalis. Andrologia, v.51, n.4, p.1-9, 2019.
LEISINGER, C.A.; PINTO, C.R.F.; CRAMER, E.; LOVE, C.C.; PACCAMONTI, D.L. Effects of Repeated Partial Thaw and Refreeze on Post-Thaw Parameters of Stallion Semen Cryopreserved in Cryovials. Journal of Equine Veterinary Science, v.49, p.19-24, 2017.
LI, S.; AO, L.; YAN, Y.; JIANG, J.; CHEN, B.; DUAN, Y.; SHEN, F.; CHEN, J.; INGLIS, B.; NI, R.; JI, W.; SI, W. Differential motility parameters and identification of proteomic profiles of human sperm cryopreserved with cryostraw and cryovial. Clinical Proteomics, v.16, n.24, p1-14, 2019.
MARTÍN-HIDALGO, D.; MACÍAS-GARCÍA, B.; GARCÍA-MARÍN, L.J.; BRAGADO, M.J.; GONZÁLEZ-FERNÁNDEZ, L. Boar spermatozoa proteomic profile varies in sperm collected during the summer and winter. Animal Reproduction Science, v.219, p.1-10, article 106513, 2020.
MARTIN RILLO, S.; MARTINEZ, E.; GARCIA ANTIGA, C.; DE ALBA, C. Boar semen evaluation in practise. Reproduction in Domestic Animals, v.31, p.519-526, 1996.
MOURA, C.S.; NUNES, A.K.S.; SILVA, B.S.; PEIXOTO, C.A.; SILVA, A.R.; SILVA, S.V.; GUERRA, M.M.P. Efeito da temperatura de descongelação na integridade de espermatozoides criopreservados de cães. Arquivo Brassileiro de Medicina Veterinária e Zootecnia, v.65, n.4, p.1057-1064, 2013.
PAQUIGNON, A.; AERGOUMN, D.; COUROT, A.; DU MESNIL DU BUISSON, F. Technologie de la congélation de la semence de verret: etude in vitro. Journée de Recherche Porcine en France, v.6, p.71-76, 1974.
PAQUIGNON, M.; BUSSIERE, J.; BARITEAU, F. Efficacité des techniques de conservation de la semence de verrat. INRA – Production Animal, v.1, p.271-280, 1988.
PEÑA, F.J.; SARAIVA, F; NÚÑES-MARTÍNEZ, I.; JOHANNISSON, A.; WALLGREN, M.; RODRIGUEZ-MARTÍNEZ, H. Do different portions of the boar ejaculate vary in their ability to sustain cryopreservation? Animal Reproduction Science, v.93, n.1-2, p.101–113, 2006.
PRIETO‐MARTÍNEZ, N.; VILAGRAN, I.; MORATÓ, R.; ÁLAMO, M.M.R.; RODRÍGUEZ‐GIL, J.E.; BONET, S.; YESTE, M. Relationship of aquaporins 3 (AQP3), 7 (AQP7), and 11 (AQP11) with boar sperm resilience to withstand freeze-thawing procedures. Andrology, v.5, n.6, p.1153-1164, 2017.
PYLES, E. Criopreservação de embriões e oócitos. Acesso: 19/08/2013. Bio Embryo; 2013. Disponível em: <http://www.bioembryo.com.br/noticias.php? cat=1&subcat=2&id=188>
RECUERO, S.; FERNANDEZ-FUERTES, B.; BONET, S.; BARRANCO, I.; YESTE, M. Potential of seminal plasma to improve the fertility of frozen-thawed boar spermatozoa. Theriogenology, v.137, n.1, p.36-42, 2019.
SICHTAR, J.; SIMONÍK, O.; BUBENÍCKOVÁ, F.; SVOBODOVÁ, J.; NEHASILOVÁ, A. Improvement in Semen Conservation of the Indigenous Czech Endangered Old Kladruber Horse: Special Focus on the Type of Extender and Packaging System. Journal of Equine Veterinary Science, v.72, p.101-107, 2019.
SILVA, A.R.; FONTENELE-NETO, J.D.; CARDOSO, R.C.S. Description of ultrastructural damages in frozen-thawed canine spermatozoa. Ciência Animal Brasileira, v.10, n.2, p.595-601, 2009.
SOUZA W.L.; MORAES E.A.; COSTA J.M.S.; SOUSA P.H.F.; LOPES JUNIOR E.S.; OLIVEIRA R.P.; TONIOLLI R. Efeito de diferentes concentrações de melatonina em espermatozoides de carneiros sobre estresse oxidativo após criopreservação. Pesquisa Veterinária Brasileira, v.36, n.7, p.657-664, 2016.
STORNELLI, M.C.; TITTARELLI, C.M.; SAVIGNONE, C.A.; STORNELLI, M.A. Efecto de los procesos de criopreservación sobre la fertilidad seminal. Análise Veterinária, v.25, n.2, p.28-35, 2005.
SUKHATO, P.; THONGSODSEANG, S.; UTHA, A.; SONGSASEN, N. Effects of cooling and warming conditions on post-thawed motility and fertility of cryopreserved buffalo spermatozoa. Animal Reproduction Science, v.67, n.1-2, p.69-77, 2001.
TONIOLLI, R. Pouvoir fecondant des spermatozoides de verrat: amelioration des conditions de conservation. 1996, 91p. These (Doctorat) - Universite Francois Rabelais de Tours - France, 1996. Resumo disponível em http://cat.inist.fr/?aModele=afficheN &cpsidt=182303.
TONIOLLI, R.; TONIOLLO, G.H.; FRANCESCHINI, P.H.; MORATO, F.M.A.C. Uso do diluente BTS no processo de congelação do sêmen suíno: II. Modificações na técnica. Ciência Animal, v.25, n.4, p.44-59, 2015.
TONIOLLI, R.; GUIMARÃES, D.B.; BARROS, T.B. Proteínas do sêmen e sua relação com a resistência à congelação em ejaculados de diferentes varrões. Revista Brasileira de Reprodução Animal, v.41, n.1, p.297-311, 2017.
TONIOLLI, R.; MOREIRA, F.R.C.; TONIOLLI, L.S.; BARROS, T.B.; GUIMARÃES, D.B.; ARAÚJO, L.R.S. Longevidade do espermatozoide suíno, incubado em diferentes tempos e temperaturas de equilíbrio. Ciência Animal, v.28, n.1, p.30-46, 2018.
VALENCIA, J.; YESTE, M.; QUINTERO-MORENO, A.; NIÑO-CARDENAS, C.P.; HENAO, F.J. Relative content of Niemann-Pick C2 protein (NPC2) in seminal plasma, but not that of spermadhesin AQN-1, is related to boar sperm cryotolerance. Theriogenology, v.45, n.15, p.181-189, 2020.
WEITZE, K.F.; STAMPA, E.; RICHTER, L.; WILLMEN, T.; WABERSKI, D. Fertility of frozen boar semen: Influence of packaging number of inseminations and seminal plasma. Reproduction in Domestic Animals, v.26, Supplement.1, p.139-142, 1991.
YESTE, M. State-of-the-art of boar sperm preservation in liquid and frozen state. Animal Reproduction, v.14, n.1, p.69-81, 2017.
YESTE, M.; RODRÍGUEZ‐GIL, J.E.; BONET, S. Artificial insemination with frozen‐thawed boar sperm. Molecular Reproduction and Development, v.84, n.9, p.802-813, 2017.
ZANIBONI, L.; CASSINELLI, C.; MAN GIAGALLI, M.G.; GLIOZZI, T.M.; CEROLINI, S. Pellet cryopreservation for chicken semen: Effects of sperm working concentration, cryoprotectant concentration, and equilibration time during in vitro processing. Theriogenology, v.82, n.2, p.251-258, 2014.
