Vol. 25 No. 4 (2015): Revista Ciência Animal
Resumo Expandido - Artigos Originais e Relatos de Caso

The BTS extender use in the swine semen freezing process: II. Modificantions in the technique

PDF (Português (Brasil))
  • R TONIOLLI,
  • G.H TONIOLLI,
  • P.H FRANCESCHINI,
  • F.M.A.C MORATO
R TONIOLLI
Faculdade de Veterinária da Universidade Estadual do Ceará (UECE)
G.H TONIOLLI
Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista
P.H FRANCESCHINI
2Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista
F.M.A.C MORATO
Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista

Published 2024-08-10

Keywords

  • Cryopreservation,
  • swine semen,
  • conservation,
  • boar

How to Cite

TONIOLLI, R.; TONIOLLI, G.; FRANCESCHINI, P.; MORATO, F. The BTS extender use in the swine semen freezing process: II. Modificantions in the technique. Ciência Animal, [S. l.], v. 25, n. 4, p. 44–59, 2024. Disponível em: https://revistas.uece.br/index.php/cienciaanimal/article/view/13572. Acesso em: 20 feb. 2026.
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

The semen of seven swine males was collected by the glove handled technique, being used the total
ejaculated. It was separate from each one a total of 10,2 x109
sptz, distributed among the treatments. The
semen quality was evaluated by the concentration (x106
sptz/mL), volume (mL), total of spermatozoas
(x109
sptz), spermatic vigour (0 to 5), movable cells (%), spermatic morphology (%) and spermatic
membrane integrity (%). The semen was diluted in Beltsville Thawing Solution. The experimental
statistic test used was the maybe blocks, with the Mann Whitney analysis, in the Lineal General Models
of Statistical Analysis System Program (SAS 6.03), with a trust interval of p<0,05. Several treatments
were proposed: differents volumes of freezing extender (18mL, 9mL, 6mL, 4mL); differents sugars
(glucose, fructose, lactose, sucrose); different cryoprotectants (glycerol, ethylene glycol, propylene
glycol, mineral oil) and use of yolk egg powdered. The smallest volume in the egg yolk extender used
without negative influence in the spermatic quality was 6mL. The glucose obtained the best results,
particularly in the percentage of intact membrane (25%). Among the cryoprotectants used, the use of the
mineral oil is inadvisable due the smaller spermatic mobility (15%) and the cells with intact membrane
(0%). The use of the egg yolk powdered can be recommended due to your best sanitary quality. In a
general way the modifications proposed by the work protocol they didn't present the expected effects.

PDF (Português (Brasil))

Downloads

Download data is not yet available.

References

  1. ALMLID, T., JOHNSON, L.A., Effects of
  2. glycerol concentration, equilibration time
  3. and temperature of glycerol 172 addition on
  4. post-thaw viability of boar spermatozoa
  5. frozen in straws. Journal of Animal Science.
  6. v.66, p.2899-2905, 1988.
  7. ANTUNES, R.C. Avanço tecnológico e
  8. aplicabilidade da técnica de congelamento
  9. de sêmen suíno. Revista Brasileira de
  10. Reprodução Animal, Belo Horizonte, v.31,
  11. n.1, p.60-63, 2007.
  12. BARRETO, M.A.P.; SILVA, J.F.S.;
  13. FAGUNDES, B.; CAIADO, J.R.C.;
  14. SOUZA, G.V. de; SHIMOYA, A. Efeito de
  15. proteínas do plasma seminal equino com
  16. massa superior a 10 kDa concentradas 10
  17. vezes sobre a congelabilidade do sêmen.
  18. Revista Brasileira de Zootecnia, v.37, n.12,
  19. p.2115-2119, 2008.
  20. BATHAGATEe, R.; MAXWELL, W,M.C.;
  21. EVANS, G. Studies on the effect of
  22. supplementing boar semen cryopreservation
  23. media with different avian egg yolk types
  24. on in vitro post-thaw sperm quality.
  25. Reproduction Domestics Animals, v.41,
  26. p.68-73, 2006.
  27. BIANCHI, I.; CALDERAM, K.;
  28. MADEIRA, E.M.; MASCHIO, E.F.;
  29. CORRÊA, E.K.; JUNIOR, T.L.;
  30. DESCHAMMPS, J.C.; CORRÊA, M.N.
  31. Crioprotetores intra e extra-celulares
  32. utilizados no congelamento do sêmen suíno.
  33. Suinocultura Industrial, n.9, p.32-36, 2006.
  34. CARDOSO, R.C.S. 2005 Características in
  35. vitro do espermatozóides canino
  36. criopreservado em água de coco.
  37. Universidade Estadual do Ceará, Fortaleza.
  38. p. (Tese de Doutorado).
  39. FERNANDES-SANTOS, M.R.; ESTESO,
  40. M.C.; SOLER, A.J.; MORTORO, V.;
  41. GARDE, J.J. Effects of egg yolk and
  42. cooling rate on the survival of refrigerated
  43. red deer (Cervus elaphus hispanicus)
  44. epidifymal spermatozoa. Reproduction
  45. Domestics Animals, v.41, p.114-118, 2006.
  46. GIL, J.; LUNDEHEIM, N.; SODERQUIST,
  47. L.; RODRIGUEZ-MARTINEZ, H.
  48. Influence of extender, temperature and
  49. addition of glycerol on post-thaw sperm
  50. parameters in RAM semen.
  51. Theriogenology, v.59, p.1241-1255, 2003.
  52. JOHNSON, L.A., WEITZE, K.F., FISER,
  53. P. MAXWELL, W.M.C. Storage of boar
  54. semen. Animal Reprodution Science, v.62,
  55. p.143-172, 2000.
  56. MAXWELL, W.M.C.; JOHNSON, L.A.
  57. Membrane status of boar spermatozoa after
  58. cooling or cryopreservation.
  59. Theriogenology, v.48, p.209-219, 1997.
  60. PENA, J.F.; JOHANNSISSON, A.;
  61. WALLGREN, M.; RODRIGUEZMARTINEZ, H. Assessment of fresh and
  62. frozen-thawed boar semen using an
  63. Annexin-V assay: a new method of
  64. evaluating sperm membrana integrity.
  65. Theriogenology, v.60, n.4, p.677-689, 2003.
  66. PHILLIPS, P.H. The preservation of bull
  67. semen. Journal of Biological Chemistry,
  68. v.130, p.415-423, 1939.
  69. POLGE, C.; SALAMON, S.; WILMUT, I.
  70. Fertilizing capacity of frozen boar semen
  71. following surgical inseminations.
  72. Veterinary Record, v.87, p.424-428, 1970.
  73. Downloaded from https://cabidigitallibrary.org by 2804:29b8:50f2:84f1:a018:937b:821f:3bdb, on 07/11/24.
  74. Subject to the CABI Digital Library Terms & Conditions, available at https://cabidigitallibrary.org/terms-and-conditions
  75. Ciência Animal 25 (4): 44-59, 2015
  76. ROBERTSON, L.; PLUMMER, J.M.;
  77. WATSON, P.F. 1988. The effect of
  78. incubation on membrane injury and calcium
  79. movement in ram spermatozoa subjected to
  80. cold shock. In Abstract of the 11th
  81. International Congress in Animal Science
  82. and Artificial Inseminations, Dublin, 1.
  83. pp.290.
  84. SALAMON, S.; MAXWELL, W.M.C.
  85. Frozen storage of ram semen. I. Processing,
  86. freezing, thawing and fertility after cervical
  87. insemination. Animal Reproduction
  88. Science, v.37, p.185-249, 1995.
  89. SALISBURY, G.W.; FULLER, H.K.;
  90. WILLET, E.L. Preservation of bovine
  91. spermatozoa in yolk-citrate diluent and field
  92. from its use. Journal of Dairy Science,
  93. v.24,p.905-910, 1941.
  94. SCHEID, I.R. Commercial swine artificial
  95. insemination in Brazil: Development and
  96. current use. Reproduction in Domestic
  97. Animals, v.26., p.299-301, 1992.
  98. SARAIVA, F.; WALLGREN, M.;
  99. JOHANNISSON, A.; RODRIGUEZMARTINEZ, H. Deep freezing of
  100. concentrated boar semen for intra-uterine
  101. insemination: effects on sperm viability.
  102. Theriogenology, v.63, p.1320-1333, 2005.
  103. SAS Statistical Analysis System. v.6.03
  104. Cary: SAS Institute, 1988. 1028p.
  105. SCHEID, I.R.; SILVEIRA, P.R.S. Uma
  106. análise da inseminação artificial na
  107. suinocultura brasileira. Suínos & Cia, v.1,
  108. n.1, p.25-28, 2002.
  109. SCHMITT, F.L.; MATTOS, R.C.; JOBIM,
  110. M.I.M.; OBERST, E.R. A concentração, a
  111. composição e a qualidade do plasma
  112. seminal na preservação do sêmen equino a
  113. +4 oC. Acta Scientiae Veterinarie. v.31, n.2,
  114. p.135-136, 2006.
  115. SCOBEY, M.J.; BIELFELD, J.S.;
  116. KRUSSEL, J.S.; JEVENDRAN, R.S. Effect
  117. of milk-yolk on the fertilizing capacity of
  118. spermatozoa. Andrologia, v.27, p.229-231,
  119. SILVA, A.R. Atualidades sobre a
  120. criopreservação do sêmen de cães. Revista
  121. Brasileira de Reprodução Animal. v.31, n.1,
  122. p.119-127, 2009.
  123. SILVA, S.V.; GUERRA, M.M.P.; Efeitos
  124. da criopreservação sobre as células
  125. espermáticas e alternativas para a redução
  126. das crioinjúrias. Revista Brasileira de
  127. Reprodução animal. v.35, n.4, p.370-384,
  128. TONIOLLI, R. Pouvoir fecondant des
  129. spermatozoides de verrat: amèlioration des
  130. conditions de conservation. 1996, 91p.
  131. These (Doctorat) - Université Francois
  132. Rabelais de Tours - France, 1996. Resumo
  133. disponível em
  134. <http://cat.inist.fr/?aModele=afficheN&cpsi
  135. dt=182303.
  136. TONIOLLI, R.; COSTA e SILVA, M.;
  137. CHAVES, R.N. Utilização de diferentes
  138. meios de ressuspensão para o sêmen do
  139. varrão após descongelação. Revista
  140. Brasileira de Ciências Veterinárias, v.16,
  141. n.1, p.41-45, 2009.
  142. WATSON, P.F. The preservation of semen
  143. in mammals. Reproductive Biology, v.1,
  144. p.283-350, 1979.
  145. WATSON, P.F. Recent developments and
  146. concepts in the cryopreservation of
  147. spermatozoa and the assessment of their
  148. post-thawing function. Reproduction,
  149. Fertility and Development, v.7, p.871-891,
  150. WATSON, P. F. The causes of reduced
  151. fertility with cryopreserved semen. Animal
  152. Reproduction Science, v.60-61, p.481-492,
  153. WOLDERS, H.; TEN NAPEL, J. Semen in
  154. straws. Pig International, v.35, n.4, p.10-14,
  155. Yi, Y.J.; CHEON, Y.M.; PARK, C.S.
  156. Effect of N-acetyl-D-glucosamine, and
  157. glycerol concentration and equilibration
  158. time on acrosome morphology and motility
  159. of frozen-thawed boar sperm

Similar Articles

You may also start an advanced similarity search for this article.