The BTS extender use in the swine semen freezing process: II. Modificantions in the technique
Keywords:
Cryopreservation, swine semen, conservation, boarAbstract
The semen of seven swine males was collected by the glove handled technique, being used the total
ejaculated. It was separate from each one a total of 10,2 x109
sptz, distributed among the treatments. The
semen quality was evaluated by the concentration (x106
sptz/mL), volume (mL), total of spermatozoas
(x109
sptz), spermatic vigour (0 to 5), movable cells (%), spermatic morphology (%) and spermatic
membrane integrity (%). The semen was diluted in Beltsville Thawing Solution. The experimental
statistic test used was the maybe blocks, with the Mann Whitney analysis, in the Lineal General Models
of Statistical Analysis System Program (SAS 6.03), with a trust interval of p<0,05. Several treatments
were proposed: differents volumes of freezing extender (18mL, 9mL, 6mL, 4mL); differents sugars
(glucose, fructose, lactose, sucrose); different cryoprotectants (glycerol, ethylene glycol, propylene
glycol, mineral oil) and use of yolk egg powdered. The smallest volume in the egg yolk extender used
without negative influence in the spermatic quality was 6mL. The glucose obtained the best results,
particularly in the percentage of intact membrane (25%). Among the cryoprotectants used, the use of the
mineral oil is inadvisable due the smaller spermatic mobility (15%) and the cells with intact membrane
(0%). The use of the egg yolk powdered can be recommended due to your best sanitary quality. In a
general way the modifications proposed by the work protocol they didn't present the expected effects.
References
ALMLID, T., JOHNSON, L.A., Effects of
glycerol concentration, equilibration time
and temperature of glycerol 172 addition on
post-thaw viability of boar spermatozoa
frozen in straws. Journal of Animal Science.
v.66, p.2899-2905, 1988.
ANTUNES, R.C. Avanço tecnológico e
aplicabilidade da técnica de congelamento
de sêmen suíno. Revista Brasileira de
Reprodução Animal, Belo Horizonte, v.31,
n.1, p.60-63, 2007.
BARRETO, M.A.P.; SILVA, J.F.S.;
FAGUNDES, B.; CAIADO, J.R.C.;
SOUZA, G.V. de; SHIMOYA, A. Efeito de
proteínas do plasma seminal equino com
massa superior a 10 kDa concentradas 10
vezes sobre a congelabilidade do sêmen.
Revista Brasileira de Zootecnia, v.37, n.12,
p.2115-2119, 2008.
BATHAGATEe, R.; MAXWELL, W,M.C.;
EVANS, G. Studies on the effect of
supplementing boar semen cryopreservation
media with different avian egg yolk types
on in vitro post-thaw sperm quality.
Reproduction Domestics Animals, v.41,
p.68-73, 2006.
BIANCHI, I.; CALDERAM, K.;
MADEIRA, E.M.; MASCHIO, E.F.;
CORRÊA, E.K.; JUNIOR, T.L.;
DESCHAMMPS, J.C.; CORRÊA, M.N.
Crioprotetores intra e extra-celulares
utilizados no congelamento do sêmen suíno.
Suinocultura Industrial, n.9, p.32-36, 2006.
CARDOSO, R.C.S. 2005 Características in
vitro do espermatozóides canino
criopreservado em água de coco.
Universidade Estadual do Ceará, Fortaleza.
p. (Tese de Doutorado).
FERNANDES-SANTOS, M.R.; ESTESO,
M.C.; SOLER, A.J.; MORTORO, V.;
GARDE, J.J. Effects of egg yolk and
cooling rate on the survival of refrigerated
red deer (Cervus elaphus hispanicus)
epidifymal spermatozoa. Reproduction
Domestics Animals, v.41, p.114-118, 2006.
GIL, J.; LUNDEHEIM, N.; SODERQUIST,
L.; RODRIGUEZ-MARTINEZ, H.
Influence of extender, temperature and
addition of glycerol on post-thaw sperm
parameters in RAM semen.
Theriogenology, v.59, p.1241-1255, 2003.
JOHNSON, L.A., WEITZE, K.F., FISER,
P. MAXWELL, W.M.C. Storage of boar
semen. Animal Reprodution Science, v.62,
p.143-172, 2000.
MAXWELL, W.M.C.; JOHNSON, L.A.
Membrane status of boar spermatozoa after
cooling or cryopreservation.
Theriogenology, v.48, p.209-219, 1997.
PENA, J.F.; JOHANNSISSON, A.;
WALLGREN, M.; RODRIGUEZMARTINEZ, H. Assessment of fresh and
frozen-thawed boar semen using an
Annexin-V assay: a new method of
evaluating sperm membrana integrity.
Theriogenology, v.60, n.4, p.677-689, 2003.
PHILLIPS, P.H. The preservation of bull
semen. Journal of Biological Chemistry,
v.130, p.415-423, 1939.
POLGE, C.; SALAMON, S.; WILMUT, I.
Fertilizing capacity of frozen boar semen
following surgical inseminations.
Veterinary Record, v.87, p.424-428, 1970.
Downloaded from https://cabidigitallibrary.org by 2804:29b8:50f2:84f1:a018:937b:821f:3bdb, on 07/11/24.
Subject to the CABI Digital Library Terms & Conditions, available at https://cabidigitallibrary.org/terms-and-conditions
Ciência Animal 25 (4): 44-59, 2015
ROBERTSON, L.; PLUMMER, J.M.;
WATSON, P.F. 1988. The effect of
incubation on membrane injury and calcium
movement in ram spermatozoa subjected to
cold shock. In Abstract of the 11th
International Congress in Animal Science
and Artificial Inseminations, Dublin, 1.
pp.290.
SALAMON, S.; MAXWELL, W.M.C.
Frozen storage of ram semen. I. Processing,
freezing, thawing and fertility after cervical
insemination. Animal Reproduction
Science, v.37, p.185-249, 1995.
SALISBURY, G.W.; FULLER, H.K.;
WILLET, E.L. Preservation of bovine
spermatozoa in yolk-citrate diluent and field
from its use. Journal of Dairy Science,
v.24,p.905-910, 1941.
SCHEID, I.R. Commercial swine artificial
insemination in Brazil: Development and
current use. Reproduction in Domestic
Animals, v.26., p.299-301, 1992.
SARAIVA, F.; WALLGREN, M.;
JOHANNISSON, A.; RODRIGUEZMARTINEZ, H. Deep freezing of
concentrated boar semen for intra-uterine
insemination: effects on sperm viability.
Theriogenology, v.63, p.1320-1333, 2005.
SAS Statistical Analysis System. v.6.03
Cary: SAS Institute, 1988. 1028p.
SCHEID, I.R.; SILVEIRA, P.R.S. Uma
análise da inseminação artificial na
suinocultura brasileira. Suínos & Cia, v.1,
n.1, p.25-28, 2002.
SCHMITT, F.L.; MATTOS, R.C.; JOBIM,
M.I.M.; OBERST, E.R. A concentração, a
composição e a qualidade do plasma
seminal na preservação do sêmen equino a
+4 oC. Acta Scientiae Veterinarie. v.31, n.2,
p.135-136, 2006.
SCOBEY, M.J.; BIELFELD, J.S.;
KRUSSEL, J.S.; JEVENDRAN, R.S. Effect
of milk-yolk on the fertilizing capacity of
spermatozoa. Andrologia, v.27, p.229-231,
SILVA, A.R. Atualidades sobre a
criopreservação do sêmen de cães. Revista
Brasileira de Reprodução Animal. v.31, n.1,
p.119-127, 2009.
SILVA, S.V.; GUERRA, M.M.P.; Efeitos
da criopreservação sobre as células
espermáticas e alternativas para a redução
das crioinjúrias. Revista Brasileira de
Reprodução animal. v.35, n.4, p.370-384,
TONIOLLI, R. Pouvoir fecondant des
spermatozoides de verrat: amèlioration des
conditions de conservation. 1996, 91p.
These (Doctorat) - Université Francois
Rabelais de Tours - France, 1996. Resumo
disponível em
<http://cat.inist.fr/?aModele=afficheN&cpsi
dt=182303.
TONIOLLI, R.; COSTA e SILVA, M.;
CHAVES, R.N. Utilização de diferentes
meios de ressuspensão para o sêmen do
varrão após descongelação. Revista
Brasileira de Ciências Veterinárias, v.16,
n.1, p.41-45, 2009.
WATSON, P.F. The preservation of semen
in mammals. Reproductive Biology, v.1,
p.283-350, 1979.
WATSON, P.F. Recent developments and
concepts in the cryopreservation of
spermatozoa and the assessment of their
post-thawing function. Reproduction,
Fertility and Development, v.7, p.871-891,
WATSON, P. F. The causes of reduced
fertility with cryopreserved semen. Animal
Reproduction Science, v.60-61, p.481-492,
WOLDERS, H.; TEN NAPEL, J. Semen in
straws. Pig International, v.35, n.4, p.10-14,
Yi, Y.J.; CHEON, Y.M.; PARK, C.S.
Effect of N-acetyl-D-glucosamine, and
glycerol concentration and equilibration
time on acrosome morphology and motility
of frozen-thawed boar sperm
