Longevity of swine sperm, incubated in different times and equilibrium temperatures
Keywords:
Conservation, semen, incubation, coolingAbstract
The swine spermatozoa are more sensitive to temperature variations due to the lipid composition of their plasma membrane. Incubation periods can affect the membrane fluidity, providing better resistance to sperm, in addition to prolonging the period of semen storage and also can increase the proportion of spermatozoa with an intact acrosome. This study aimed to identify the best relation between temperature and incubation time of diluted swine semen. There were used 57 ejaculates from 6 boars divided into 05 treatments: T1 (control): Semen diluted in Beltsville Thawing Solution (BTS) at 35 °C 17 ° C (7 days); T2: Diluted semen (35 ºC) 30 ºC / 4 hours 17 ºC (7 days); T3: Diluted semen (35 ºC) 25 ºC / 4 hours 17 ºC (7 days); T4: Diluted semen (35 ° C) 30 ° C / 1 hour 25 ° C / 4 hours 17 ° C (7 days); T5: Diluted semen (35 ° C) 30 ° C / 1 hour 25 ° C / 1 hour 20 ° C / 4 hours 17 ° C (7 days). On the day of collection, in the first 8 hours weren’t observed differences between treatments (p> 0.05). In D0, values of vigor and motility of T5 were higher than T1 (p <0.05), and in D5 T1 was higher than T5 in relation to the same parameters (p <0.05). There were no differences between treatments (p>0.05), only between post-dilution semen and T3, T4 and T5 (P <0.05). Therefore, it was concluded that the previous incubation did not increase the storage time of the semen and did not bring losses during its conservation. There were statically analyzed: vigor, motility (Mann-Whitney), where in DO, T5 was superior to T1 (p<0.05), and in D5 and D9, T1 was superior to T5 (p<0.05), and morphology (“t” of Student Test), where there were not any differences between treatments. In all treatments, were found differences between pure semen and the diluted, and between the diluted and the following analyses (p<0.05). Therefore, conclusions is that incubation didn’t increase the time of semen preservation and that dilution moment is the most critical in the cooling process of the cooled semen.
References
ALKMIN, D.V.; SILVA FILHO, J.M.; PALHARES, M.S.; SIQUEIRA, A.P.; MACHADO, G.S.; SILVA, C.L.A.; TARANTINI, T.C. Efeito da porção do ejaculado e do método de resfriamento sobre as características físicas do sêmen suíno. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.63, n.6, p.1287-1294, 2011.
ALMEIDA, M.C.S. Impacto da diluição isotérmica e bitérmica sobre a qualidade do semen suíno. 2014. Dissertação (Mestrado em Ciências Veterinárias) Universidade Federal do Rio Grande do Sul, Porto Alegre, 2014. 49p.
ALTHOUSE, G. C.; WILSON, M. E.; KUSTER, C.; PARSLEY, M. Characterization of lower temperature storage limitations of fresh-extended porcine semen. Theriogenology, v.50, p.535-543, 1998.
ARAÚJO, L.R.S, BARROS, T.B., GUIMARÃES, D.B., CANTANHÊDE, L.F., DIAS, A.V., TONIOLLI, R. Uso de diluentes e temperaturas alternativas na conservação prolongada do sêmen do varrão. Ciência Animal Brasileira, v.17, n.1, p.26-35, 2016.
BARROS, T.B.; GUIMARÃES, D.B.; CANTANHÊDE, L.F.; DIAS, A.V.; SOUZA, L.P.; FEUGANG, J.M.; TONIOLLI, R. Leite em pó desnatado como diluente alternativo na etapa de resfriamento durante protocolo de congelação do sêmen suíno. Semina: Ciências Agrárias, Londrina, v.36, n.3, supl.1, p.2023-2030, 2015.
BAMBA, K.; CRAN, D.G. Further studies on rapid dilution and warming of boar semen. Journal of Reproduction Fertility, v.82, p.509-518, 1988.
BORTOLOZZO, F.P.; WENTZ, I.; DALLANORA, D. Situação atual da inseminação artificial em suínos. Acta Scientifiae Veterinariae, v.33, n.1, p.17-32, 2005.
BUHR, M.M. Preservation of boar sperm alters membrane molecular dynamics. Proceeding in 2th International Conference on Boar Semen Press, Beltsville-USA, p 81-93, 1990.
BWANGA, C.O. Cryopreservation of boar sperm. Acta Veterinariae Scandinavae, v.32, p.431-453, 1991.
CÂMARA, D. R.; GUERRA, M. M. P. Refrigeração e criopreservação do sêmen ovino: danos inerentes à técnica e influência da suplementação do meio com antioxidantes sobre a qualidade espermática. Revista Brasileira de Reprodução Animal, v.35, p.33-40, 2011.
CASTELO, T.S. Efeito dos processos de centrifugação, diluição e descongelação sobre a qualidade do semen de catetos (Tayassu tgacu, Linnaeus, 1758). 2010. Dissertação (Mestrado em Ciência Animal) – Universidade Federal Rural do Semi-árido, Mossoró, 2010. 100p.
COLÉGIO BRASILEIRO DE REPRODUÇÃO ANIMAL. Manual para exame andrológico e avaliação de sêmen animal, 3ª ed., Belo Horizonte, 2013. 104p.
COURTENS, J.L.; PAQUIGNON, M. Ultraestructure of fresh, frozen, and frozen-thawd spermatozoa of the boar. Proceeding in 2th International Conference on Boar Semen Pres., Beltsville-USA, p.61-87, 1990.
ERIKSSON, B.M.; VAZQUEZ, J.M.; MARTINEZ, E.A.; ROCA, J.; LUCAS, X.; RODRÍGUEZ-MARTINEZ, H. Effects oh holding time during cooling and of type of package on plasma membrane integrity, motility and in vitro oocyte penetration ability of frozen-thawed boar spermatozoa. Theriogenology, v.55, p.1593-1605, 2001.
GUIMARÃES, D.B.; BARROS, T.B., CANTANHÊDE, L.F., FEUGANG, J.M.N., SOUZA, L.P., TONIOLLI, R. Qualidade espermática durante a curva de resfriamento do sêmen suíno diluído em água de coco em pó visando sua criopreservação. Ciência Animal Brasileira, v.19, p.1-16, 2018.
HUO, L. MA, X., YANG, Z. Assessment of sperm viability, mitochondrial activity, capacitation and acrossome intactness in extended boar semen during long-term storage. Theriogenology, v.8632, p.1-12, 2002.
JOHNSON, L.A.; WEITZE, K. F.; FISER, P.; MAXWELL, W.M.C. Storage of boar semen. Animal Reproduction Science, v.62, p.143-172, 2000.
KATZER, L.H. Resfriamento de sêmen suíno: efeito da temperatura de armazenamento, incubação prévia, taxa de resfriamento e diluentes. Acta Scientiae Veterinariae, v.37, n.1, p.97-98, 2009.
KATZER, L.H.; BORTOLOZZO, F.P.; WENTZ, I.; BERNARDI, M.L.; PEIXOTO, C.H.; VIDOR, R.M. Viabilidade do sêmen suíno armazenado a 12 ºC e 5 ºC após incubação a 17 ºC. In: X Congresso Brasileiro de Veterinários Especialistas em Suínos, Anais, Anais, v.2, Porto Alegre-RS, p.241-242, 2001a.
KATZER, L.H.; BERNARDI, M.L.; PADILHA, A.P.; BORTOLOZZO, F.P.; WENTZ, I.; COSTI, G.; DIEHL, G.N. Diferentes temperaturas e diluentes no resfriamento do sêmen suíno. In: X Congresso Brasileiro de Veterinários Especialistas em Suínos, Anais, v.2, Porto Alegre-RS, p.243-244, 2001b.
KATZER, L.H.; BORTOLOZZO, F.P.; WENTZ, I.; POSTAL, A.T.; PADILHA, A.P.; LECZNIESKI, L.F.; BERNARDI, M.L. Armazenamento de sêmen suíno a 5 ºC de acordo com o período de incubação e a taxa de resfriamento. In: X Congresso Brasileiro de Veterinários Especialistas em Suínos, Anais, Anais, v.2, Porto Alegre-RS, p.245-246, 2001c.
KOTZIAS-BANDEIRA, E. Influência de diferentes diluentes e temperaturas de refrigeração sobre a qualidade de sêmen suíno. Brazilian Journal of Veterinary Research and Animal Science, v.36, n.4, p.75-83, 1999.
KOMMISRUD, E., PAULENZ, H., SEHESTED, E., GREVLE, I.S. Influence of Boar and Semen Parameters on motility and acrosome integrity in liquid boar semen stored for five days. Acta Veterinaria Scandinavica, v.43, p.49-55, 2002.
KUMUS S.A. (Ed.) Manual de inseminácion artificial porcina. Madrid (Espanha), 1993. 81p.
LALRINTLUANGA, K.; DEKA, B.C.; NATH, K.C.; HMAR, L.; BHUYAN, D.; BISWAS, R.K. Effect of different extenders on the quality of boar semen during preservation at 18°C. International Journal of Multidisciplinary Approach & Studies. v.3, n.1, p.224232. 2016.
LIMA, L.F.; MOURA, P.; PASSOS, P.I.B.; LEAL, D.R.; RUMPF, R.; NEVES, J.P.. Influência de sistemas de refrigeração sobre a qualidade do sêmen ovino criopreservado em palhetas. Ciência Animal Brasileira, v.11, n.4, 2010.
LÓPEZ RODRIGUEZ, A.; RIJSSELAERE, T.; VYT, P.; VAN SOON, A.; MAES, D. Effect of dilution temperature on boar semen quality. Reproduction in Domestic Animals, v.47, n.5, p.63-66, 2011.
MAES, D.; LÓPEZ RODRIGUES, A.; RJSSELAERE, T.; VYT, P.; VAN, S.A. Artificial insemination in pigs. Artificial insemination in farm animals, v.1, p.74-79, 2011.
MARTIN RILLO, S.; MARTINEZ, E.; GARCIA ANTIGA, C.; DE ALBA, C. Boar Semen evaluation in practise. Reproduction in Domestic Animal, v.31, p.519-526, 1996.
MAXWELL, W.M.C.; JOHNSON, L.A. Membrane status of boar spermatozoa after cooling or cryopreservation. Theriogenology, v.48, p.209-219, 1997.
MEDRANO, A.; WATSON, A.; HOLT, W.V. Importance of cooling rate and animal variability for boar sperm cryopreservation: insights from the cryomicroscope. Reproduction, v.123, n.2, p.315-322, 2002.
NASCIMENTO, E.F.; VALE FILHO, V.R.; SILVA FILHO, J.M.; NASCIMENTO, J.F.K. Efeito da taca de resfriamento sobre a motilidade, sobrevivência e morfologia espermática de varrões. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.50, n.6, p.691-694, 1998.
POLGE, C. Artificial insemination in Pigs. Veterinary Record, v.68, n.4, p.62-75, 1956.
PURSEL, V.G.; JOHNSON, L.A. Distribution of glutamic oxaloacetic transaminase dehydrogenase activities in boar semen after cold shock and freezing. Cryobiology, v.7, p.141-144, 1970.
PURSEL, V.G.; JOHNSON, L.A.; RAMPACEK, G.B. Acrossome morfology of boar spermatozoa incubated before cold shock. Journal of Animal Science, v.34 n.2, p.278-283, 1972.
PURSEL, V.G.; JOHNSON, L.A.; SCHUMAN, L.L. Fertilizing capacity of boar semen stored at 15 ºC. Journal of Animal Science, v.35, n.2, 157-161, 1973a.
PURSEL, V.G.; SCULMAN, L.L.; JOHNSON, L.A. Effect of holding time on storage of boar spermatozoa at 5 ºC. Journal of Animal Science, v.35, n.3, p.371-277, 1973b.
RIESENBECK, A. Review on international trade with boar semen. Reproduction in domestic Animals, v.46, suppl.2, p.1-3, 2011.
SCHULZE, M., HENNING, H., RÜDIGER, K., WALLNER, U., WABERSKI, D. Temperature management during semen processing: Impact on boar sperm quality under laboratory and field conditions. Theriogenology, v.80, n.9, p.990-998, 2013.
SCHULZE, M.; RÜDIGER, K.; JUNG, M.; GROSSFELD, R. Use of refractometry as a new management tool in AI boar center for quality assurance of extender preparations. Animal Reproduction Science, v.152, p.77-82, 2015.
SILVA M.C., OLIVEIRA MOURA L.C., VAZ DE MELO M.I., MELO MAMBRINI J.V., NEVES M.M., HENRY M.R.J.M., SNOECK P.P.D.N. Prolonged post cooling but not pre-cooling equilibrium length improves the viability of ram sperm cryopreserved in an extender containing low-density lipoproteins. Small Ruminant Research, v.119, n1-3 ,p.88-95, 2014.
TONIOLLI, R. Pouvoir fecondant des spermatozoides de verrat: amelioration des conditions de conservation. 1996, 91p. These (Doctorat) - Universite Francois Rabelais de Tours - France, 1996. Resumo disponível em <http://cat.inist.fr/?aModele=afficheN&cpsidt=182303>.
ZOU, C.X.; YANG, Z.M. Evaluation on sperm quality of freshly ejaculeted boar semen during in vitro storage under different temperatures. Theriogenology, v.53, p.1477-1488, 2000.
