PRESENCE OF THE VIRAL DNA IN OOPHOROUS CUMULUS COMPLEXES OF COWS CLINICALLY HEALTHY AND NATURALLY INFECTED BY BOVINE HERPESVIRUS 1 (BOHVl)
Parole chiave:
oocytes, Nested-PCR, viral infectionAbstract
The objective of this study was to verify the presence of bovine herpesvirus 1 (BoHV l) DNA in Cumulus Oophorus Complexes (COCs) and blood from naturally infected cows. COCs were obtained from 1S donors using ultrasound-guided follicular aspiration (OPU). DNA extraction was performed on a pool of COCs from all aspirations from the same donor and on whole blood. After this procedure, the Nested-PC R reactions were carried out. BoHVl DNA was identified in the pool of COCs from three seropositive donors, and was not detected in any of the seronegative samples. However, no viral DNA was found in blood samples, even those from seropositive animals. All blood samples and COCs were submitted to Nested-PCR for the detection of BoHV S, with no positive results being found. The results obtained allow us to conclude that viral DNA may be present in ovarian structures of cows naturally infected by BoHV 1.
Riferimenti bibliografici
ABDELMAGID , O.Y.; MINOCHA , H.C.; COLLINS, J.K.; CHOWDHURY ,
S.I. Fine mapping of bovine herpesvirns-1 (BoHV-1) glycoprotein D (gD) neutralizing epitopes by type specific monoclonal antibodies and sequence compaiison with BoHV-5 gD. Virology, v.206, p.242-253 , 1995.
BIELANSKI, A.; DUBUC, C. ln vitro
fertilization and culture of ova from heifors infocted with bovine herpesvirns-1 (BHV-1). The1iogenology, v.41, p.1211-1217 , 1994.
CAMPOS, F.S.; FRANCO, A.C.; HÜBNER, S.O.; OLIVEIRA, M.T.;
SILVA, A.D.; ESTEVES , P.A.; ROEHE, P.M.; RIJSEWIJK, F.A.M. High prevalence of co-infoctions with bovine herpesvirns 1 and 5 found in catle in southem Brazil. Veterinary Microbiology , v.139, p.67-73 , 2009.
CHOWDHURY , S.I. Moleculai·basis of antigenic vanat1011 between the glycoprotein e of respirato1y bovine herpesvirus 1 (BHV-1) and neurovirulent BHV- 5. Virology, v.213 , p.558-568 , 1995.
FERREIRA, C.Y.M.R.; PIATTI, R.M.; MIYASHIRO, S.; GALUPPO, A.G.; ZERIO, N.M.C.; SÂMARA, S.I.; D'ANGELO , M. Ocorrência do herpesvirus bovino 1 (BoHV-1) no líquido folicular e células epiteliais de oviduto bovino . Arq uivos do Instituto Biológico, v. 72, p.309-311 , 2005.
