Effect of adding zinc oxide nanoparticles to the freezing extender of ram semen
Keywords:
Nanotechnology, Ram, CryopreservationAbstract
The aim of this study was to evaluate the effect of adding zinc oxide nanoparticles to the ram semen cryopreservation diluter. Semen pools (n=6), from three Santa Inês breeders, were diluted in Tris-yolk (5% glycerol), supplemented with zinc oxide nanoparticles (0, 25, 75 and 150μg/mL) at a concentration of 200x106 sperm/mL. The samples were frozen in an automated system and stored in liquid nitrogen (-196 °C). At the time of analysis, the semen samples were thawed (37 °C/30s) and evaluated for sperm kinetics, plasma and acrosomal membrane integrity and mitochondrial membrane potential. There was no significant difference (p>0.05) between the experimental groups in the parameters of kinetics, plasma membrane and acrosome integrity. As for the potential of mitochondrial membrane, all treated groups were significantly (p≤0.05) larger than the control. It was concluded that the zinc oxide nanoparticles increase the mitochondrial membrane potential of ram sperm submitted to the freezing/thawing process.
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