Use of Aloe vera extract in swine semen criopreservation
Keywords:
Semen swine, freezing, conservation, cryoprotectant, Aloe veraAbstract
Egg yolk is used as a cryoprotective agent in semen freezing diluents for protection against thermal shock, despite the possibility of microbiological contamination. Authors search for Aloe vera, an alternative medium for the conservation of semen. The objective was to evaluate the use of the cryoprotectant Aloe vera in the cryopreservation of swine semen. We used 25 ejaculates, collected through the gloved hand technique. The samples were
diluted in the coolant diluent (10.0% egg yolk, Aloe vera 10, 20 and 30%) at 17 °C, freezing diluent (refrigerant diluent + glycerol 6.0%) at 5 °C and stored in vales. The vanes were evaluated for vigor, motility, membrane functionality, vitality, acrosome integrity and assisted semen analysis (CASA). The data were evaluated for normality, then analyzed using ANOVA and multiple comparisons, the analyzes were performed with a significance level of 5.0%, using the program R3.4.0. Descriptive statistics were used for data on vigor, motility and CASA. In the evaluation of thawed semen for membrane functionality, acrosome integrity and vitality, the highest values were found for the egg yolk samples (p<0.05), and among the samples that used Aloe vera, found significant differences between the different concentrations tested (p>0.05). The data of vigor, motility and CASA, of the samples using Aloe vera presented values of 0.0. The results showed that the different concentrations of Aloe vera did not exert the desired cryoprotective effect on the sperm cell, so it is understood that further studies are needed on its action on semen conservation, as well as a possible interaction with swine spermatozoa. On the other hand, it is believed that additional studies on the cooling curve of the porcine semen are still necessary, aiming at its cryopreservation.
References
ANTUNES, L.P.; BRITO, B.F.; RODRIGUES, F.R.N.; CATUNDA, A.G.V.; RIOS,
R.R.S. Avaliação do sêmen ovino resfriado e diluído em ACP 101/102 adicionado de diferentes proporções de Aloes. Acta Veterinária Brasílica, v.8, suppl. 2, p.232-233, 2014.
BARIL, G.; CHEMINEAU, Y.; COGNIE, Y.; GUÉRIN, B.; LEBŒUF, B.; ORGEUR, P.;
VALLET, J.C. Manuel de formation pour l’insémination artificielle chez les ovins et les caprins. 3a ed., Nouzilly, INRA, 1993. 137p.
BARROS, M.H.C.; SHIOMI, H.H.; AMORIM, L.S.; GUIMARÃES, S.E.F.; LOPES, P.S.;
SIQUEIRA, J.B.; GUIMARÃES, J.D. Criopreservação de sêmen de suínos da raça Piau submetido a três protocolos de congelamento. Revista Brasileira de Zootecnia, v.41, n.4, p.914-922, 2012.
BARROS, T.B.; GUIMARÃES, D.B.; CANTANHÊDE, L.F.; DIAS, A.V.; SOUZA, L.P.;
FEUGANG, J.M.; TONIOLLI, R. The use of skimmed dried milk as an alternative diluent for the cooling step during the boar sêmen freezing procedure. Semina: Ciências Agrárias, v.36, Suppl. 1, p.2023-2030, 2015.
BERGERON, A.; CRÊTE, M.H.; BRINDLE, Y.; MANJUNATH, P. Low-Density
Lipoprotein Fraction from Hen's Egg Yolk Decreases the Binding of the Major Proteins of Bovine Seminal Plasma to Sperm and Prevents Lipid Efflux from the Sperm. Biology of Reproduction, v.70, n.3, p.708-717, 2004.
BRITO, B.F.; ANTUNES, L.P.; RODRIGUES, F.R.N.; SALGUEIRO, C.C.M.;
CAVALCANTE, J.M.M.; NUNES, J.F. Efeito do Aloe vera adicionado em diferentes concentrações à água de coco em pó (ACP-102®) no sêmen ovino diluído e incubado por duas horas. Acta Veterinária Brasílica, v.8, Suppl. 2, p.242-243, 2014.
BROEKHUIJSE, M.L.W.J.; ŠOŠTARIĆ, E.; FEITSMA, H.; GADELLA, B.M.
Application of computer-assisted semen analysis to explain variations in pig fertility. Journal of Animal Science, v.90, n.3, p.779-789, 2012.
CAO, H.; SUN, X.; LI, Q.; ZHOU, S.; NAN, X.; HU, J.; WANG, L.; HE, Y. Testing
Rhodiola sachalinensis saccharide as cryoprotectant for bovine spermatozoa. Journal of Dairy Science, v.96. n.11, p.6965-6972, 2013.
CASAS, I.; SANCHO, S.; BALLESTER, J.; BRIZ, M.; PINART, E.; BUSSALLEU, E.; YESTE, M.; FÀBREGA, A.; RODRÍGUEZ-GIL, J.E.; BONET, S. The HSP90AA1 sperm
content and the prediction of the boar ejaculate freezability. Theriogenology, v.74, n.6, p.940-950, 2010.
CHOI. S.; CHUNG, M.H. A review on the relationship between Aloe vera components and their biologic effects. Seminars in Integrative Medicine, v.1, n.1, p.53-62, 2003.
Colégio Brasileiro Reprodução Animal. Manual para exame andrológico e avaliação de sêmen animal. 3a ed., Belo Horizonte: CBRA. pp.47-50, 2013.
CRESPILHO, A.M.; SÁ FILHO, M.F.; DELL'AQUA Jr, J.A.; NICHI, M.; MONTEIRO,
G.A.; AVANZI, B.R.; MARTINS, A.; PAPA, F.O. Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders. Livestock Science, v.149, n.1, p.1-6, 2012.
DAIGNEAULT, B.W.; MCNAMARA, K.A.; PURDY, P.H.; KRISHER, R.L.; KNOX,
R.V.; RODRIGUEZ-ZAS, S.L.; MILLER, D.J. Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment. Andrology, v.3, n.3, p.558-568, 2014.
ESTRADA, E.; RODRÍGUEZ-GIL, J.E.; ROCHA, L.G.; BALASCH, S.; BONET, S.;
YESTE, M. Supplementing cryopreservation media with reduced glutathione increases
fertility and prolificacy of sows inseminated with frozen-thawed boar semen. Andrology, v.2, n.1, p.88-100, 2013.
FARSTAD, W. Cryopreservation of Canine Semen – New Challenges. Reproduction in Domestic Animal, v.44, n.2, p.336-341, 2009.
GUTIÉRREZ, A.J.; COSME, R.W.; JIMÉNEZ, C.J.A.; RAMIREZ, G.J.A. Agua de coco,
suero fetal bovino, Aloe vera y sus combinaciones para criopreservar semen ovino. Archivos de Zootecnia, v.55, n.209, p.101-104, 2006.
KARAGEORGIOU, M.A.; TSOUSIS, G.; BOSCOS, C.M.; TZIKA, E.D.; TASSIS, P.D.;
TSAKMAKIDIS, I.A. A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility. Acta Veterinaria Brno, v.85, n.1, p.23-31, 2016.
KONG, D.; SHANG, H.; GUO, K.; LIU, Y.; ZHANG, J.; WEI, H. A study on optimizing
the cryopreservation methods for Bama miniature pig semen. Experimental Animals, v.61, n.5, p.533-42, 2012.
MELO-MACIEL, M.A.P.; LEITE-CASTRO, L.V.; LEITE, J.S.; OLIVEIRA, M.S.; ALMEIDA-MONTEIRO, P.S.; NUNES, J.F.; SALMITO-VANDERLEY, C.S.B. Aloe
vera na criopreservação do sêmen de tambaqui (Colossoma macropomum). Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.67, n.3, p.945-949, 2015.
MOUSSA, M.; MARTINET, V.; TRIMECHE, A.; TAINTURIER, D.; ANTON, M. Low
density lipoproteins extracted from hen egg yolk by na easy method: cryoprotective effect on fronzen - thawed bull semen. Theriogenology, v57, n.6, p.1695 -1706, 2002.
PANDEY, A.; SINGH, S. Aloe vera: A systematic review of its industrial and ethno- medicinal efficacy. International Journal of Pharmaceutical Research and Allied Science, v.5, n.1, p.21-33, 2016.
PARRILLA, I.; DEL-OLMO, D.; SIJSES, L.; MARTINEZ-ALBORCIA, M.J.; CUELLO,
C.; VAZQUEZ, J.M.; MARTINEZ, E.A.; ROCA, J. Differences in the ability of spermatozoa from individual boar ejaculates to withstand different semen-processing techniques. Animal Reproduction Scienve, v.132, n.1-2, p.66-73, 2012.
PINHO, R.O.; LIMA, D.M.; SHIOMI, H.H.; SIQUEIRA, J.B.; SILVA, H,T.; LOPES,
P.S.; GUIMARÃES, S.E.; GUIMARÃES, J.D. Effect of different cryo-protectants on the viability of frozen/thawed semen from boars of the Piau breed. Animal Reproduction Science, v.146, n.3-4, p.187-192, 2014.
RONDON, R.M.M.; ARAÚJO, A.A.; TONIOLLI, R.; RONDON, F.C.M. Perspectivas
sobre o uso de antioxidantes na conservação do sêmen de suínos. Revista Brasileira de Reprodução Animal, v.36, n.3, p.141-147, 2012.
RUNGRUANGSAK, J.; SUWIMONTEERABUTR, J.; BURANAAMNUAY, K.;
PONGPENG, J.; TUMMARUK, P. Association between frozen-thawed boar sperm motility characteristics assessed by Sperm Class Analyzer (SCA®) and sperm viability assessed by SYBR-14/EthD-1. Thai Journal of Veterinary Medicine, v.47, Suppl.1. p.7-8, 2017.
SAVI, P.A.P.; ZAVAREZ, L.B.; KIPPER, B.H.; FELICIANO, M.A.R.; VICENTE,
W.R.R.; OLIVEIRA, M.E.F. Uso de antioxidantes em meios diluidores para sêmen ovino: revisão de literatura. ARS Veterinaria, v.31, n.1, p.12-18, 2014.
SOUZA, A.L.; LIMA, G.L.; PEIXOTO, G.C.; SILVA, A.M.; OLIVEIRA, M.F.; SILVA,
A.R. Use of Aloe vera–based extender for chilling and freezing collared peccary (Pecari tajacu) semen. Theriogenology, v.85, n.8, p.1432 -1438, 2016.
SURJUSHE, A.; VASANI, R.; SAPLE, D.G. Aloe vera: A short review. Indian Journal of Dermatology, v.53, n.4, p.163-166, 2008.
TONIOLLI, R.; BARROS, T.B.; TONIOLLI, L.S.; GUIMARÃES, D.B.; DIAS, A.V.;
CANTANHÊDE, L.F.; ARAÚJO, L.R.S.; FILHO, I.B.Q. Qualidade espermática do ejaculado suíno conservado a 5 ºC no diluente ACP-103® associado à gema de ovo. Ciência Animal, v.23, n.2, p.45-57, 2013.
TONIOLLI, R.; GUIMARÃES, D.B.; BARROS, T.B. Proteínas do semen e sua relação com a resistência à congelação em ejaculados de diferentes varrões. Revista Brasileira de Reprodução Animal, v.41, n.1, p.297-311, 2017.
WATSON, P.F. Recent developments e concepts in cryopreservation of spermatozoa e the assessment of their post-thawing function. Reproduction, Fertility and Development, v.7, n.4, p.871-891, 1995.
YESTE, M. State-of-the-art of boar sperm preservation in liquid and frozen state. Animal Reproduction Science, v.14, n.1, p.69-81, 2017.
