USO DA BROMELINA NA QUALIDADE DO SÊMEN CAPRINO CONGELADO/DESCONGELADO
Keywords:
Goat breeding, Sperm, MotilityAbstract
The objective of this study was to evaluate the effect of bromelain on sperm quality, after defrosting, in goats. For this purpose, five Anglo Nubian goats were used. Semen collection was performed with the aid of an artificial vagina. The semen was evaluated for its macroscopic and microscopic parameters. After the analysis, the total volume of the pool was divided into three groups. One belonging to the control group (ACP-101/102®) and two treatment groups with ACP-101/102® enriched with bromelain in concentrations of 5% and 10%. The samples were cryopreserved with the aid of the Tk3000 device. Then, the straws were immersed in liquid nitrogen and stored in cryogenic cylinders. After one week the samples were thawed and evaluated by the system CASA (Computer Assisted Sperm Analysis). After thawing, the total sperm motility assessed by the system CASA was preserved in the control group and in the treatment group at a concentration of 5%. The curvilinear speed (LCV) and mean trajectory speed (VAP) of sperm were higher in the control group compared to the others. Thus, it is concluded that bromelain does not present genotoxicity to goat sperm, as well as its use is satisfactory in terms of the quality of seminal parameters after thawing.
References
BALA, M. Bromelain production: current trends and perspective. Archives des Sciences, v.65, p.369-399, 2012.
BROMELAIN. Monograph. Alternative Medicine Review, v.15, p.361-368, 2010.
COLÉGIO BRASILEIRO DE REPRODUÇÃO ANIMAL (CBRA). Manual para Exame Andrológico e Avaliação de Sêmen Animal, 3ª Ed., Belo Horizonte, 2013. 104p.
MILLER C.D. Optimizing the use of frozen-thawed equine semen. Theriogenology, v.70, p.463-468, 2008.
MIRANDA, Í.K. Obtenção, caracterização e atividade antitumoral in vitro de bromelina de diferentes partes de abacaxizeiros. Dissertação (Mestrado Acadêmico em Recursos Genéticos Vegetais) - Universidade Estadual de Feira de Santana, Feira de Santana, 2014.
NASCIMENTO, J.N.; BLUME, H.; OLIVEIRA, F.J.G.; OLIVEIRA, R.A. Utilização de diferentes diluentes na criopreservação de espermatozoides de garanhões mangalarga marchador. Ciência Animal Brasileira, v.16, n.3, p.324-330, 2015.
SAYALI, K.; SADICHHA, P.; SUREKHA, S. Microbial esterases: an overview. International Journal of Current Microbiology and Applied Sciences, v.2, p.135-146, 2013.
SILVA, K.M.G.; GAMBOA, S.C.; RODRIGUES, A.S; RAMALHO, J.S.; PESSOA GUERRA, M.M. Adição de piruvato de sódio e trolox ao diluidor utilizado para congelação de sêmen de garanhões férteis e subférteis. Ciência Rural, v.38, p.2271-2277, 2008.
