Molecular detection of groups of fermentative bacteria in the rumen of cattle and buffaloes in Santarém-Pa
Keywords:
Molecular biology, ribosomal RNA, ruminantsAbstract
The objective of this work was to detect groups of bacteria in the rumen content of buffaloes and cattle using molecular biology techniques. The samplings were carried out in the Fridge Mararu, in the city of Santarém-PA. Were collected 10 samples of ruminal fluid, being 5 to buffaloes and 5 of cattle. DNA extraction was performed according to the protocol of the Kit Brasílica based on silica. For the detection of bacteria, we used primers 16S L2 and 1472, AR and BR. It was used for PCR, a volume of 25 µl PCR kit Mix LGC 2X. The samples were run on 3% agarose gel stained with bromophenol blue and GelRed. The gel was visualized on a UV radiation. The primers used in the amplification of the region of the 16S rRNA gene was shown to be effective for the studies of detection of ruminal bacteria. It was observed the difference between cattle and buffalo, where the primers L2 and 1472 amplified in cattle the group of fibrolytic bacteria and in buffaloes the group of not fibrolytic bacteria. While the primers AR and BR amplified the group of not fibrolytic bacteria for cattle and the group of fibrolytic bacteria to Buffalo. The work of identification of ruminal bacteria in buffaloes is scarce, so the importance of the results found. There was a difference in the groups of microorganisms found in ruminants between buffaloes and cattle, and the type of primer used, showing the importance of the study of bacteria in the rumen. The study requires a larger approach, such as the sequencing of the DNA samples to better identify the species present within the different groups were detected. Other Molecular Biology techniques can be used for better accuracy of data found, such as the use of PCR in real time.
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