Separation of Nelore bull seminal plasma proteins by gel filtration chromatography and their associations with andrological parameters
Keywords:
Chromatograph, proteins, seminal plasma, induced acrossome reaction, sperm, ZebuAbstract
Chromatographic profile of seminal proteins from 16 young Nelore bulls, and their associations with andrologic parameters, semen freezing and induction acrossome reactions were evaluated. Total seminal plasma protein concentrations ranged from 3.18 to 52.7 mg/mL, average of 26.8±20.5 mg/mL. The seminal plasma protein profile by gel filtration showed eight different fractions. The highest protein peaks were those with molecular weight ranging from 640-240 kDa, 187-70 kDa to 12.4-4.7 kDa, and were present in more than 80% of the bulls. The peak with a variation between 54.7-20.4 kDa also presented a good protein proportion and frequency of occurrence among the bulls (81%). According to the literature, this is the peak with most interest in exploration of proteins associated with fertility and semen cryopreservation success. Sperm concentration was more associated with the highest molecular weight peak (between 187-70 kDa) and sperm motility and freezability were closer to protein groups with molecular weight less than 10 kDa. The result of induced acrosome reaction (RAI) showed no association with any protein groups. The separation of seminal plasma protein groups according to their molecular weight by superose gel 12 was an important tool for the initial analysis of the protein profile of Nellore bulls, and its use as an initial procedure for any scientific research aiming at explore the protein profile of seminal plasma of bulls.
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