Vol. 29 No. 4 (2019): Revista Ciência Animal
Artigos Originais

Separation of Nelore bull seminal plasma proteins by gel filtration chromatography and their associations with andrological parameters

PDF (Português (Brasil))
  • Daniel Fábio SALVADOR,
  • Luiz Altamiro Garcia NOGUEIRA,
  • Sandro César SALVADOR
Daniel Fábio SALVADOR
Fundação Centro de Ciências e Educação Superior a Distância do Rio de Janeiro (CECIERJ)
Luiz Altamiro Garcia NOGUEIRA
Universidade Federal Fluminense
Sandro César SALVADOR
Universidade Federal Fluminense

Published 2019-12-31

Keywords

  • Chromatograph,
  • proteins,
  • seminal plasma,
  • induced acrossome reaction,
  • sperm,
  • Zebu
    • ...More
    Less

How to Cite

SALVADOR, D. F.; NOGUEIRA, L. A. G.; SALVADOR, S. C. Separation of Nelore bull seminal plasma proteins by gel filtration chromatography and their associations with andrological parameters. Ciência Animal, [S. l.], v. 29, n. 4, p. 18–31, 2019. Disponível em: https://revistas.uece.br/index.php/cienciaanimal/article/view/9727. Acesso em: 30 jan. 2026.
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Chromatographic profile of seminal proteins from 16 young Nelore bulls, and their associations with andrologic parameters, semen freezing and induction acrossome reactions were evaluated. Total seminal plasma protein concentrations ranged from 3.18 to 52.7 mg/mL, average of 26.8±20.5 mg/mL. The seminal plasma protein profile by gel filtration showed eight different fractions. The highest protein peaks were those with molecular weight ranging from 640-240 kDa, 187-70 kDa to 12.4-4.7 kDa, and were present in more than 80% of the bulls. The peak with a variation between 54.7-20.4 kDa also presented a good protein proportion and frequency of occurrence among the bulls (81%). According to the literature, this is the peak with most interest in exploration of proteins associated with fertility and semen cryopreservation success. Sperm concentration was more associated with the highest molecular weight peak (between 187-70 kDa) and sperm motility and freezability were closer to protein groups with molecular weight less than 10 kDa. The result of induced acrosome reaction (RAI) showed no association with any protein groups. The separation of seminal plasma protein groups according to their molecular weight by superose gel 12 was an important tool for the initial analysis of the protein profile of Nellore bulls, and its use as an initial procedure for any scientific research aiming at explore the protein profile of seminal plasma of bulls.

PDF (Português (Brasil))

Downloads

Download data is not yet available.

References

  1. ASSUNÇÃO, T.I., FONTES, W., SOUZA, M.V., RICART, C.A.O. Perfil de proteínas do
  2. plasma seminal de touros Nelore e sua relação com a fertilidade. Revista Brasileira de
  3. Reprodução Animal, v.27, n.2, p.187-188, 2003.
  4. ASSUNÇÃO, T.I.; FONTES, W.; SOUZA, M.V.; RICART, C.A.O. Proteome Analysis of
  5. Nelore Bull (Bos taurus indicus) Seminal Plasma. Protein & Peptide Letters. v.12, n.8, 2005.
  6. BERNECIC, N.; GADELLA, B.; LEAHY, T.; DE GRAAF, S. Novel methods to detect
  7. capacitation-related changes in spermatozoa. Theriogenology, v.137, p.56-66, 2019.
  8. BELLIN, M.E.; HAWKINS, H.E.; AX, R.L. Fertility of range beef bulls grouped according to
  9. presence or absence of Heparin-binding proteins in sperm membranes and seminal fluid. Journal
  10. of Animal Science, v.72, p.2441-2448, 1994.
  11. CHACUR, M.G.M.; RABESQUINE, M.M.; PARRA, J.A.G.; MACHADO NETO, N.B.;
  12. RABESQUINE, M.M.; PARRA, J.A.G. Seleção da fertilidade em touros e proteínas do plasma
  13. seminal, correlação com o quadro espermático. Revista Brasileira de Reprodução Animal, v.27,
  14. n.2, p.185-186, 2003.
  15. COLÉGIO BRASILEIRO DE REPRODUÇÃO ANIMAL (CBRA). Manual para exame
  16. andrológico e avaliação de sêmen animal. Belo Horizonte: CBRA, 2013. 103p.
  17. DACHEUX, J.L.; DACHEUX, F. Protein Secretion in the Epididynis. In: The Epidymis: From
  18. Molecules to Clinical Practice. 1a ed., by Robaire and Hinton, p.151-167, 2002.
  19. DALTON, J.C.; DERAGONB, L.; VASCONCELOS, J.L.M.; LOPES, C.N.; PERES, R.F.G.;
  20. AHMADZADEH, A. Fertility-associated antigen on Nelore bull sperm and reproductive
  21. outcomes following first-service fixed-time AI of Nelore cows and heifers. Theriogenology,
  22. v.77, p.389–394, 2012.
  23. DRUART, X.; GRAAF, S. Seminal plasma proteomes and sperm fertility. Animal Reproduction
  24. Science. v.194, p.33-40, 2018.
  25. DIDION, B.A.; DOBRINSKY, J.R.; GILES, J.R.; GRAVES, C.N. Staining procedures to detect
  26. viability and the true acrossome reaction in spermatozoa of various species. Gamete Reserch,
  27. v.22, p.51-57, 1989.
  28. FELICIANO SILVA, A.E.D. Reação acrossômica induzida: método indicador de fertilidade de
  29. touros. Brasília: Embrapa Recursos Genéticos e biotecnologia, 1998. 38p. (Embrapa recursos
  30. genéticos. Documentos, 35).
  31. FERNANDES, C.E.; SOUZA, F F.; SOUZA-NETO, J.A.; RIBOLA, P.E.M. Heparin-binding
  32. proteins of seminal plasma in Nellore bulls. Ciência Rural, v.39, n.1. 2009.
  33. HAFEZ, E.S.E. Reprodução Animal. 7ª ed., São Paulo: Ed. Manole, 2004. 513p.
  34. JOBIM, M.I.M.; OBERST, E.R.; SALBEGO, C.G.; SOUZA, D.O.; WALD, V.B.;
  35. TRAMONTINA, F.; MATTOS, R.C. Two-dimensional polyacrylamide gel electrophoresis of
  36. bovine seminal plasma proteins and their relation with semen freezability. Theriogenology, v.61,
  37. p.255-266, 2004.
  38. JUDEZ, A.L. Técnicas de Análisis de dados multidimensionales. Ministério da Agricultura,
  39. Pesca y Alimentacion. 1989. 301p.
  40. KILLIAN, G.J.; CHAPMAN, D.A.; ROGOWSKI, L.A. Fertility associated proteins in Holstein
  41. bull seminal plasma. Biology of Reproduction, v.49, p.1202-1207, 1993.
  42. KUMARESAN, A.; JOHANNISSON, A.; HUMBLOT, P.; BERGQVIST, A.S. Effect of bovine
  43. oviductal fluid on motility, tyrosine phosphorylation, and acrosome reaction in cryopreserved
  44. bull spermatozoa. Theriogenology, v.124, p.48-56, 2019.
  45. KUMAR, P.; KUMAR, D.; SINGH, I.; YADAV, P.S. Seminal Plasma Proteome: Promising
  46. Biomarkers for Bull Fertility. Agricultural Research, v.1, n.1, p.78–86, 2012.
  47. LI, C.; WANG, D.; ZHOU, X. Sperm proteome and reproductive technologies in mammals.
  48. Animal Reproduction Science, v.173, p.1-7, 2016.
  49. LOWRY, O.H.; ROSENBROUGH, N.J.; FARS, A.L.; RANDALL, R.J. Proteins measurement
  50. with the folin phenol reagent. Journal of Bioligy and Chemistry, v.193, p.265-275, 1951.
  51. MANN, T.; MANN, C.L. Male Reproductive Function and Semen. 1a ed., New York. Ed:
  52. Springer-Verlag, p.1-37, 1981.
  53. MANJUNATH, P.; CHANDONNET, L.; LEBLOND, E.; DESNOYERS, L. Major proteins of
  54. bovine seminal vesicles bind to spermatozoa. Biol. Reprod., v.48, p.27-37, 1993.
  55. MAGALHÃES Jr, M.J.; MARTINS, L.F.; SENRA, R.L.; SANTOS, T.F.; OKANO, D.S.;
  56. PEREIRA, P.R.; FARIA-CAMPOS A.; CAMPOS, S.V.; GUIMARÃES, J.D.; BARACATPEREIRA,
  57. M.C. Differential abundances of four forms of Binder of SPerm 1 in the seminal
  58. plasma of Bos taurus indicus bulls with different patterns of semen freezability. Theriogenology,
  59. v.86, n.3, p.766-777, 2016.
  60. MAXWELL, W.M.; DE GRAAF, S.P.; GHAOUI REL, H.; EVANS, G. Seminal plasma effects
  61. on sperm handling and female fertility. Society of Reproduction and Fertility, v.64, p.13-38,
  62. suppl., 2007.
  63. PARK, Y.J.; PANG, W.K.; RYU, D.Y.; SONG, W.H.; RAHMAN, M. S.; PANG, M.G.
  64. Optimized combination of multiple biomarkers to improve diagnostic accuracy in male fertility.
  65. Theriogenology, v.139, p.106-112, 2019.
  66. REGO, J.P.A.; CRISP, J.M.; MOURA, A.A.; NOUWENS, A.S.; LI, Y.; VENUS, B.; CORBET,
  67. N.J.; CORBET, D.H.; BURNS, B.M.; BOE-HANSEN, G.B.; MCGOWAN, M.R. Seminal
  68. plasma proteome of electroejaculated Bos indicus bulls. Animal Reproduction Science, v.148, 1-
  69. , p.1-17, 2014.
  70. RICKARD, J.P.; POOL, K.R.; DRUART, X., GRAAF, S.P. The fate of spermatozoa in the
  71. female reproductive tract: A comparative review. Theriogenology, v.137, p.104-112, 2019.
  72. RONCOLETTA, M.; MORANI, E.; ESPER, C.R.; BARNABE, V.H.; FRANCESCHINI, P.H.
  73. Fertility-associated proteins in Nelore bull sperm membranes. Animal Reproduction Science,
  74. v.91, p.77-87, 2006.
  75. RONCOLETTA, M.; MORANI, E.; RODRIGUES, L. H.; FRANCHESCHINI, P. H.
  76. Proteômica para investigar a congelabilidade do semen bovino. Brazilian Journal of Veterinary
  77. Research and Animal Science, v.50, n.1, p.43-49, 2013.
  78. RODRÍGUEZ-MARTÍNEZ, H.; KVIST, U.; ERNERUDH, J.; SANZ, L.; CALVETE, J.J.
  79. Seminal plasma proteins: what role do they play? American Journal of Reproduction and
  80. Immunology, v.66, p.11–22, 2011.
  81. SAMPAIO, I.B.M. Relatório das atividades de pós-doutorado desenvolvidas no período de
  82. /09/92 a 04/03/93. Madrid: Universidade politécnica de Madrid, 1993. 123p.
  83. SILVA, A.E.D.F.; DIAS, A.L.; UNANIAN, M.M. Conteúdo de peptídeos e avaliação
  84. morfofisiológica dos espermatozoides do epidídimo e ejaculado de bovinos. Revista Brasileira
  85. de Zootecnia, v.32, p.1890-1900, 2003.
  86. SENEDA, M.M.; SILVA-SANTOS, K.C.; MARINHO, L.S.R. Biotechnology of animal
  87. reproduction. Londrina State University, Londrina, Paraná. 1a ed., Hauppauge, New York: Nova
  88. Science Publisher's, Inc. 2016. 348p.
  89. SUAREZ S.S. Mammalian sperm interactions with the female reproductive tract. Cell Tissue
  90. Research, v.363, p.185-194, 2015.

Most read articles by the same author(s)