Hyperlipid diet induces NAFLD associated liver damage without redox imbalance in Swiss mice liver
Keywords:
Fatty liver disease, Hyperlipid diet, Redox imbalance, MiceAbstract
Non-alcoholic fatty liver disease (NAFLD) is an increasingly prevalent disease strongly associated with redox imbalance and loss of glycemic homeostasis. The development of in vivo models that mimics its pathophysiological characteristics is essential for the discovery and development of therapeutic strategies. To validate a NAFLD model associated with redox imbalance and low-fat diet-induced glucose intolerance and easily reproducible. Sixteen Swiss mice received a high fat diet for 10 weeks. Throughout the experiment, body weight, glucose tolerance, oxidative stress markers and liver morphology were evaluated. The hyperlipid diet promoted a significant increase in triglyceride levels (p<0.05) and liver tissue weight (p<0.01), in addition to severe inflammation, necrosis and steatosis of hepatocytes. The animals that followed the hyperlipidic diet (HD) still had a lower glucose tolerance (p<0.05), however it did not show reduction of thiol groups. The HD-induced NAFLD model was associated with liver histological damage and glucose intolerance, however it did not present redox imbalance, being an unfeasible model for studies with antioxidant treatments. The study aimed to validate a model of NAFLD induced by a high-fat diet associated with changes in the glycemic response and without changes in redox homeostasis in mice.
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