Vol. 30 No. 1 (2020): Revista Ciência Animal
Artigos Originais

Use of 3% DMSO and different glycerol concentrations in the swine semen cooling curve

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  • Ludymila Furtado Cantanhêde,
  • Daianny Barboza Guimarães,
  • Tatyane Bandeira Barros,
  • Aline Viana Dias,
  • Lina Raquel Santos Araújo,
  • Ricardo Toniolli
Ludymila Furtado Cantanhêde
Programa de Pós-Graduação em Ciências Veterinárias da Universidade Estadual do Ceará (UECE)
Daianny Barboza Guimarães
Programa de Pós-Graduação em Ciências Veterinárias da Universidade Estadual do Ceará (UECE)
Tatyane Bandeira Barros
Programa de Pós-Graduação em Ciências Veterinárias da Universidade Estadual do Ceará (UECE)
Aline Viana Dias
Programa de Pós-Graduação em Ciências Veterinárias da Universidade Estadual do Ceará (UECE)
Lina Raquel Santos Araújo
Programa de Pós-Graduação em Ciências Veterinárias da Universidade Estadual do Ceará (UECE),
Ricardo Toniolli
Laboratório de Reprodução Suína e Tecnologia de Sêmen (UECE)

Published 2020-12-31

Keywords

  • Cryoprotector agents,
  • freezing,
  • sperm,
  • boar

How to Cite

CANTANHÊDE, L. F.; GUIMARÃES, D. B. .; BARROS, T. B. .; DIAS, A. V.; ARAÚJO, L. R. S. .; TONIOLLI, R. Use of 3% DMSO and different glycerol concentrations in the swine semen cooling curve. Ciência Animal, [S. l.], v. 30, n. 1, p. 47–63, 2020. Disponível em: https://revistas.uece.br/index.php/cienciaanimal/article/view/9651. Acesso em: 30 jan. 2026.
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

This study aimed to test the cryoprotectant Dimethylsulfoxide (DMSO-3%) and glycerol, in five concentrations (0.5%, 1%, 3%, 5% and 7%) in a new cooling curve. Seven collections from seven boars (n=49) through the gloved hand technique were made. DMSO (3%) and glycerol were added separately to the freezing diluent. During the analysis of the curve, the lowest mean sperm count was observed at a concentration of 7% glycerol. The concentration of 1% glycerol obtained more sperm motility (72±12.3) but, did not differ in concentrations of 5% glycerol. Higher mean sperm motility and vigor after thawing were observed at 3% glycerol, differing from 0.5% glycerol and 3% DMSO concentrations regarding vigor and other treatments regarding sperm motility. In the osmotic resistance test, 3% glycerol as a freezing agent provided better protection to the spermatic membrane, differing from the

 

other treatments except for 3% DMSO and 1% glycerol. A higher percentage of living cells was observed in the 5% and 7% glycerol concentration, differing from the other concentrations. The highest percentage of spermatozoa with intact acrosomes was observed when the freezing diluent containing 3% glycerol (73.8±11.9) was used. The results of this study suggested that glycerol is still the best cryoprotective option to be used in cryopreservation processes of swine sperm.

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