HEMOTROPIC MYCOPLASMA IN DOMESTIC CATS FROM THE CENTRAL REGION OF RIO GRANDE DO SUL STATE, BRAZIL
Keywords:
Hemoplasma, polymerase chain reaction, catAbstract
Hemotropic mycoplasmas, also known as hemoplasmas, are bacteria that attach to the red blood cells and are the causative agents of feline infectious anemia (AIF). AIF can be mild or severe and can eventually lead to death. The hemoplasmas have been described in several countries, including Brazil; however, studies on the frequency of infection in central Rio Grande do Sul state is yet unknown. The aim of this study was to report the prevalence of hemoplasmas in this region as well as to evaluate the hematological changes and risk factors such age and sex, associated with infection. For this, 192 blood samples from domestic cats were collected and analyzed by species-specific conventional
polymerase chain reaction (PCR) assays. Overall, 14,6% cats were infected by at least one hemoplasma, but structures morphologically compatible with hemoplasmas were not identified in blood smears. Concerning age, animals aged ≤3 years had a higher incidence of infection than cats older than 3 years of age with 75% of infected samples being males. There was no statistical difference in hematological variables between infected and uninfected cats. Thus, we conclude that the prevalence of hemotropic mycoplasmas in Central Rio Grande do Sul state is 14.6% and that infection was not correlated with the presence of anemia and young male cats were more likely to be infected.
References
AGUIRRE, D.H.; THOMPSON, C.; NEUMANN, R.D., SALATIN, A.O; GAIDO, A.B.;
DE ECHAIDE, S.T. Clinical mycoplasmosis outbreak due to Mycoplasma ovis in sheep from Shalta, Argentina. Clinical, microbiological and molecular diagnosis. Revista Argentina de Microbiologia, v.41, n.4, p.212–214, 2009.
BARKER, E.; TASKER S. Haemoplasmas: lessons learned from cats. The New Zealand Veterinary Journal, v.61, n.4, p.184-92, 2013.
BAUER, N.; BALZER, H.J.; THÜRE S.; MORITZ, A. Prevalence of feline haemotropic mycoplasmas in convenience samples of cats in Germany. Journal Feline Medicine Surger, v.10, n.3, p.252–258, 2008.
BENNETT, A.D.; GUNN-MOORE, D.A.; BREWER, M.; LAPPIN, M.R. Prevalence of
Bartonella species, haemoplasmas and Toxoplasma gondii in cats in Scotland. Journal Feline Medicine Surgery, v.13, n.8, p.553–557, 2011.
BIONDO, A.W.; SANTOS A.P.; GUIMARÃES, A.M.; VIEIRA, R.F.C.; VIDOTTO, O.; MACIEIRA, D.B; ALMOSNY, N.R.P.; MOLENTO, M.B.; TIMENETSKY, J.; MORAIS,
H.A.; GONZÁLEZ, F.H.D.; MESSICK, J.B. A review of the occurrence of hemoplasmas (hemotropic mycoplasmas) in Brazil. Revista Brasileira de Parasitologia Veterinária, v.18, n.3, p.1–7, 2009.
CETINKAYA, H.; HAKTANIR, D.; ARUN, S.; VURUSANER, C. Molecular detection
and prevalence of feline hemotropic mycoplasmas in Istanbul, Turkey. Acta Parasitologica, v.61, n.1, p.165–171, 2016.
DEAN, R.S.; HELPS, C.R.; GRUFFYDD JONES, T.J.; TASKER, S. Use of real-time
PCR to detect Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' in the saliva and salivary glands of haemoplasma-infected cats. Journal Feline Medicine Surgery, v.10, n.4, p.413–417, 2008.
DUARTE, A.; MARQUES, V.; CORREIA, J.H.D.; BRÁZ, B.S.; RODRIGUES, C.; MARTINS, T.; ROSADO, R.; FERREIRA, J.P.; SANTOS-REIS, M.; TAVARES, L.
Molecular Detection of Haemotropic Mycoplasma Species In Urban And Rural Cats From Portugal. Journal Feline Medicine Surgery, v.15, n.6, p.516–522, 2015.
FELDMAN, B.F.; ZINKL, J.G.; JAIN, N.C. Schalm's veterinary hematology. 5a ed., Philadelphia, Lippincott Williams & Wilkins, 2000. 787p.
GIROTTO-SOARES, A.; SOARES, J.F.; BOGADO, A.L.G.; MACEDO, C.A.B.;
SANDESKI, L.M.; GARCIA, J.L.; VIDOTTO, O. ‘Candidatus Mycoplasma haemobos’: Transplacental transmission in dairy cows (Bos taurus). Veterinary Microbiology, v.195, p.22-24, 2016.
HARBUTT, P.R.A. A clinical appraisal of feline infectious anaemia and its Transmission under natural conditions. Australian Veterinary Journal, v.39, p.401-404, 1963.
HARVEY, J.W. Hemotrophic mycoplasmosis (Hemobartonellosis). In: GREENE, C.E. Infectious Diseases of the Dog and Cat, 3rd ed., Saint Louis, MO, Elsevier Inc, p.252–260, 2006.
HORNOK, S.; MICSUTKA, A.; MELI, M.L.; LUTZ, H.; HOFMANN-LEHMANN, R.
Molecular investigation of transplacental and vector-borne transmission of bovine haemoplasmas. Veterinary Microbiology, v.152, p.411–414, 2011.
JENKINS, K.S.; DITTMER, K.E.; MARSHALL, J.C.; TASKER, S. Prevalence and risk
factor analysis of feline haemoplasma infection in New Zealand domestic cats using a real- time PCR assay. Journal feline Medicine and Surgery, v.15, n.12, p.1063–1069, 2013.
MACIEIRA, D.B.; MENEZES, R.C.; DAMICO, C.B.; ALMOSNY, N.R.; MCLAN, H.L.;
DAGGY, J.K.; MESSICK, J.B. Prevalence and risk factors for hemoplasmas in domestic cats naturally infected with feline immunodeficiency virus and/or feline leukemia virus in Rio de Janeiro, Brazil. Journal Feline Medicine Surgery, v.10, n.2, p.120–129, 2008.
MESSICK, J.B. Hemotrophic mycoplasmas (hemoplasmas): a review and new insights into pathogenic potential. Veterinary Clinical Pathology, v.33, n.1, p.2–13, 2004.
MUSEUX, K.; BORETTI, F.S.; WILLI, B.; RIOND, B.; HOELZLE, K.; HOELZLE, L.E.;
WITTENBRINK, M.M.; TASKER, S.; WENGI, N.; REUSCH, C.E.; LUTZ, H.;
HOFMANN-LEHMANN, R. In vivo transmission studies of 'Candidatus Mycoplasma turicensis' in the domestic cat. Veterinary Research, v.40, n.5, p.40–45, 2009.
NEIMARK, H.; JOHANSSON, K.E.; RIKIHISA, Y.; TULLY, J.G. Proposal to transfer
some members of the genera Haemobartonella and Eperythrozoon to the genus Mycoplasma with descriptions of ‘Candidatus Mycoplasma haemofelis’, ‘Candidatus Mycoplasma haemomuris’, ‘Candidatus Mycoplasma haemosuis’ and ‘Candidatus Mycoplasma wenyonii’. International Journal Systematic and Evolutionary Microbiology, v.51, p.891–899. 2001.
REBOUÇAS, N.A. Biologia Molecular Aplicada À Medicina. Disponível em:
<http://www.i2bio.org/wp-content/uploads/BIOLOGIA-MOLECULAR-APLICADA-A- MEDICINA-FUNDAMENTOS-TEORICOS-E-METODOLOGICIOAS. pdf> Acesso em:
de dezembro de 2018.
ROSENQVIST, M.B.; MEILSTRUP, A.K.H.; LARSEN, J.; OLSEN, J.E.; JENSEN, A. L.;
THOMSEN, L.E. Prevalence of feline haemoplasma in cats in Dennamark. Acta Veterinaria Scandinavica, v.58, n.1, p.78, 2016.
SANTOS, A.P.; MESSICK, J.B.; BIONDO, A.W.; OLIVEIRA, S.T; PEDRALLI, V.; LASTA, C.S.; LACERDA, L.A.; ESTEVES, V.S.; HOFMANN-LEHMANN, R.; WILLI,
B.; GONZÁLEZ, F.H.D. Design, optimization, and application of a conventional PCR assay with an internal control for detection of ‘Candidatus Mycoplasma turicensis’ 16S rDNA in domestic cats from Brazil. Veterinary Clinical Pathology, v.38, n.4, p.443–452, 2009.
SANTOS, A.P.; GUIMARAES, A.M.S.; DO NASCIMENTO, N.C.; SANMIGUEL, P.J.;
MARTIN, S.W.; MESSICK, J.B. Genome of Mycoplasma haemofelis, unraveling its strategies for survival and persistence. Veterinary Research, v.42, n.1, p.102, 2011.
SANTOS, A.P.; CONRADO, F.O.; MESSICK, J.B.; BIONDO, A.W.; OLIVEIRA, S.T.; GUIMARAES, A.M.S.; DO NASCIMENTO, N.C.; PEDRALLI, V.; LASTA, C.S.;
GONZÁLEZ, F.H.D. Hemoplasma prevalence and hematological abnormalities associated with infection in three different cat populations from Southern Brazil. Revista Brasileira de Parasitologia Veterinária, v.23, n.4, p.428–434, 2014.
SYKES, J.E.; TERRY, J.C.; LINDSAY, L.L.; OWENS, S.D. Prevalences of various
hemoplasma species among cats in the United States with possible hemoplasmosis. Journal American Veterinary Medical Association, v.232, n.3, p.372–379, 2008.
SYKES, J.E. Feline hemotropic mycoplasmas. Veterinary Clinics of North America: Small Animal Practice, v.40, n.6, p.1157–70, 2010.
SINEREY, K.S.; SAMPAIO-JUNIOR, F.D.; SOUSA, L.O.; SANTOS, R.C.; GONÇALVES, E.C.; SCOFIELD, A.; GÓES-CAVALCANTE, G. Diagnóstico molecular
da infecção por hemoplasmas em gatos domésticos naturalmente infectados da cidade de Belém, Pará. Pesquisa Veterinária Brasileira, v.33, n.9, p.1116–1120, 2013.
TANAHARA, M.; MIYAMOTO, S.; NISHIO, T.; YOSHII, Y.; SAKUMA, M.; SAKATA, Y.; NISHIGAKI, K.; TSUJIMOTO, H.; SETOGUCHI, A.; ENDO, Y. An
epidemiological survey of feline hemoplasma infection in Japan. The Journal of Veterinary Medical Science, v.72, n.12, p.1575–1581, 2010.
TASKER, S. Hemobartonellafelis. In: Lappin, M.R. Segredos em medicina interna felina. 1ª ed., Porto Alegre, Artmed, p.455–459, 2004.
TASKER, S. Haemotropic mycoplasmas: what’s their real significance in cats? Journal Feline Medicine Surgery, v.12, n.5, p.369–81, 2010.
WILLI, B.; BORETTI, F.S.; CATTORI, V.; TASKER, S.; MELI, M.L.; REUSCH, C.;
LUTZ, H.; HOFMANN-LEHMANN, R. Identification, molecular characterization, and experimental transmission of a new hemoplasma isolate from a cat with hemolytic anemia in Switzerland. Journal of Clinical Microbiology, v.43, n.6, p.2581–2585, 2005.
WILLI, B.; BORETTI, F.S.; MELI, M.L.; BERNASCONI, M.V.; CASATI, S.; HEGGLIN, D.; PUORGER, M.; NEIMARK, H.; CATTORI, V.; WENGI, N.; REUSCH,
C.E.; LUTZ, H.; HOFMANN-LEHMANN, R. Real-time PCR investigation of potential vectors, reservoirs, and shedding patterns of feline hemotropic mycoplasmas. Applied and Environmental Microbiology, v.73, n.12, p.3798–802, 2007.
