Morphological and morphometric evaluation of femal e mice uterus after using soursop extract
Keywords:
Medicinal plants, Antineoplastic agents, ToxicityAbstract
The present study aimed to evaluate the effect of Annona muricata extract on the uterus of female mice by macro and microscopic analyzes. Twenty female mice were randomly assigned to four groups: control (saline solution), cyclophosphamide, and soursop leaf extract at two different concentrations (50 and 100 mg/kg). After seven days of the experiment, the females were euthanized and their wombs were collected and submitted for to macroscopic analysis, followed by histological processing for morphological and morphometric analyses of uterine layers and endometrial glands. The macroscopic characteristics of the uterus were maintained in all groups evaluated. Microscopically, no pathological changes were identified in the uterine cells. However, there was a significant increase in the myometrium layer after the treatment with cyclophosphamide (p<0.05). The internal endometrial glands diameter was significantly smaller in both groups treated with A. muricata extract compared to the control group (p<0.05), while the total glands diameter was significantly smaller only at the highest concentration of A. muricata extract compared to the control group (p<0.05). The administration of A. muricata leaf extract did not cause morphological changes to the uterus. Thus, the extract did not present uterine toxicity under the conditions evaluated.
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