EFFECT OF HIGH INTENSITY INTERVA L TRA INING IN PERFORMANCE STRESS TEST

Abstract

The high-intensity interval training (HllT) is characterized by vigorous and intermittent performing certain exercise separated by periods of passive or active rest with low intensity. This training model can be as effective as continuous endurance training. However, the aim of this study was to investigate the effect of HllT in fitness, in addition to checking the lactate curve and glycemic variation in maximal exercise test (MET). For this, used were 8 male Wistar rats on a treadmill adapted for rodents for two weeks . To determine the intensity of the animais were subjected to TEM before the start of training and subsequently refreshed every two weeks. lt HAS consists of steps 3 minutes of running with constant load, followed by two minutes of passive rest. At each stage there was an increase of 0.3 km / h to exhaustion of the animal and determine the lactate threshold. Blood samples (25 µL) were performed during rest, with the help of capillary calibrated with EDTA. lmmediately after collecting the blood was transferred to eppendorf tube containing 50 µL of sodium fluoride for subsequent analysis lactimeter YSI 2300 STAT plus. The glucose analysis pre and post TEM was performed with the aid of reactive tapes and analyzed Accu-Chek ACT IVE®. After a certain MET, the animais were submitted to HllT, which consisted of five minutes of warming, followed by four shots at 80% of maximum capacity followed by passive rest, finishing with five minute coai down. This study was approved by the Ethics Committee on Animal Use (CEUA) of the State University of Ceará (UECE) by the number 2542310/2015 . For analysis of the linearity of lactate curves was carried out Pearson's correlation for ali MET. We used the analysis of variance (ANOVA - two way) followed by Bonferroni post test to check differences in pre and post glucose MET. ln ali cases the significance levei was preset at p <0.05 . An increase in the physical condition of the animais, where the maximum speed obtained in the first MET was 1.94 ± 0.12 and 3.95 ± 0.24 after 8 weeks of HllT with Pearson linear correlation was significant in ali MET . This is due to ATP production by anaerobic glycolytic system result in the accumulation of pyruvate and NADH in the muscle, which subsequently forms the lactate. ln glycemic analysis it was found that in the first TEM there was a significant increase in post­ workout blood glucose (pre=119±19, post=170±19), possibly by increased activation of the adrenergic system inducing hepatic glycogenolysis . ln TEM 5 there was a significant reduction in blood glucose after TEM (pre=137±15, post=130±21) can be explained by increased phosphorylation of GLUT4, increasing glucose uptake by muscle exercise. Therefore, HllT was able to increase physical ability of animais to give an increasing curve of lactate in ali MET and distinct variations in glycaemia.