Sperm cooling of dogs with prostatomegaly
Keywords:
Cryopreservation, Semen, Prostatomegaly, DogAbstract
A prostatomegaly is one of the most common prostate disease in non-castrated dogs. Studies that describe coolled semen of these animals do not exist. So, the present work aimed to cool the semen of dogs with prostatomegaly. Four dogs (2 Rottweiler, 1 German Shepherd and 1 Labrador Retriever) were diagnosed with prostatomegaly by rectal palpation, ultrasound examination and hemospermia. Two semen samples were collected from each animal (n = 8) by digital manipulation, using optical microscopy for sperm concentration, motility (%), vigor (0-5), morphologically normal spermatozoa (%), viable (%) and functional membrane (%). After fresh semen (FS) evaluation, the semen was diluted with TRIS added with 20% egg yolk and 6% glycerol, producing a concentration of 50 x106 sptz/mL. (4 °C/40min) (after dilution - AD), transferred to a refrigerator (4 °C) and reassessed at 2h, 4h and 6h. A motility and vigor of FS were the most advanced, but remained and did not differ from AD up to 6h refrigeration. Viability did not differ between FS and AD but reduced approximately 52.5% during refrigeration. The percentage of spermatozoa with functional membrane and normal morphology did not differ between the times analyzed. As the parameters expected during refrigeration are inferior to those recommended for a species (<30% and 3, respectively), it is concluded that semen of dogs with prostatomegaly are invalid and unsatisfactory for assisted reproduction programs.
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