SEMINAL PARAMETERS OF TRANSGENIC AND NON TRANSGENIC GOATS FOR THE HUMAN GRANULOCYTE COLONY FACTO

Authors

  • Beatriz Mano e SILVA Laboratório de Fisiologa e Controle da Reprodução, Faculdade de Veterinária, (UECE)
  • Luiz Antonio Moreira MIRANDA Laboratório de Fisiologa e Controle da Reprodução, Faculdade de Veterinária, (UECE)
  • Thais Thatiane dos santos SOUZA Laboratório de Fisiologa e Controle da Reprodução, Faculdade de Veterinária, (UECE)
  • Wasim Al SHEBLI Laboratório de Fisiologa e Controle da Reprodução, Faculdade de Veterinária, (UECE)
  • Luciana Magalhães MELO Laboratório de Fisiologa e Controle da Reprodução, Faculdade de Veterinária, (UECE)
  • Vicente José de Figueirêdo FREITAS Laboratório de Fisiologa e Controle da Reprodução, Faculdade de Veterinária, (UECE)

Keywords:

transgenc bucks, semen, spermatozoa, recombinant protein

Abstract

The goat species has become an excellent animal model aiming at the production of recombinant proteins in milk. Obtaining transgenic founder males is important, as they make it possible to increase the number of transgenic offspring by simply using artificial insemination. The objective of this work was to compare the seminal parameters of male transgenic (TG) and non-transgenic (NTG) goats for the human Granulocyte Colony Stimulating Factor (hG-CSF). For this purpose, weekly semen samples were collected from all animals through an artificial vagina. The following parameters were evaluated: volume, mass motility, sperm concentration, progressive individual motility (MIP), vigor and sperm pathologies. Statistical differences were observed only for volume and MIP, which had higher values ​​(p<0.05) in TG animals. It was also observed, in both groups, a small percentage of spermatic pathologies (<2%), with no statistical difference (p>0.05). In conclusion, the presence of the transgene did not affect semen quality, favoring and contributing to the formation of a transgenic herd.

References

HAMMER,RE.; PURSEL VG;REXROAD CE JR WALL,RJ ; DJ EBERT, KM; PALMITER, RD; BRINSTER, R.L. Production of transgênicos rabbits, Sherlock and pigs by microinjection. Nature, v.315, p.680-683,1985.

KEEFER, C.L.Production of bioproducts through the use of transgenic animal models.

Animal Reproduction science, v.82-83, p.5-12,2004.

LEBOEUF, B; RESTALL, B; SALAMON, S. Production and storage of goat semen for artificial insemination. Animal Reproduction Science, v.62, p.113-141,2000.

SMITH, C. Hematopoietic stem cells and hematopoiesis. Cancer control, v.10,p.9-16,2003.

WHEELER, M.B. production of transgenic livestock: promise fulfilled. Journal of Animal Science, v.18,p.32-37,2003.

WALL, RJ; KERR, D.E; BONDIOLI,K.R. Trangenic dairy cattle: genetic engineering on a large scale. Journal of Dairy Science, v.80, p.2213-2224,1992.

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Published

2023-08-04

How to Cite

E SILVA, B. M.; MIRANDA, L. A. M.; SOUZA, T. T. dos santos; SHEBLI, W. A.; MELO, L. M.; FREITAS, V. J. de F. SEMINAL PARAMETERS OF TRANSGENIC AND NON TRANSGENIC GOATS FOR THE HUMAN GRANULOCYTE COLONY FACTO. Ciência Animal, [S. l.], v. 29, n. 1, p. 30–36, 2023. Disponível em: https://revistas.uece.br/index.php/cienciaanimal/article/view/11055. Acesso em: 23 nov. 2024.

Issue

Vol. 29 No. 1 (2019): Revista Ciência Animal

Section

Artigos Originais

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