Factors influencing the conception rate and pregnancy loss in Girolando embryos produced in vitro

Authors

  • Melissa Defensor LOBATO Faculdade de Medicina Veterinária da Universidade Federal de Uberlândia (UFU)
  • Flávio Luiz Alves MARQUES Médico Veterinário Autônomo
  • Mayara Mafra SOARES Faculdade de Medicina Veterinária da Universidade Federal de Uberlândia (UFU)
  • Ana Cláudia Fagundes FARIA Faculdade de Medicina Veterinária da Universidade Federal de Uberlândia (UFU)
  • Gustavo Pereira CADIMA Faculdade de Medicina Veterinária da Universidade Federal de Uberlândia (UFU)
  • Ricarda Maria dos SANTOS Faculdade de Medicina Veterinária da Universidade Federal de Uberlândia (UFU)

Keywords:

ETFT, Blastocyst, IVP, Bovine, Pregnancy

Abstract

The in vitro production of embryos is fundamental importance for livestock, although it is still a biotechnology with varieds results than expected, being therefore necessary study ways to improve its rates. The objective of this study was to evaluate if the recipient category, season and stage of embryo development affect conception rates and gestational losses in recipients of girolando’s embryos produced in vitro and transferred at fixed time (ETFT). The experiment was carried out in a commercial dairy farm, located in the Uberlândia - MG region. A total of 1,017 TETF were evaluated. The recipients were divided into lactating cows (n=618), dry cows (n=44) and heifers (n=355). The seasons were grouped in summer (spring and summer) and winter (autumn and winter). The embryos were grouped in Group 1 (n=216) - initial blastocyst (BI) and blastocyst (BL) and Group 2 (n=801) - expanded blastocyst (BX), hatching blastocyst (BH) and hatched (BE). Receptor category effect was detected at conception rate (p<0.001) and loss of gestation (p<0.003). The summer affected negatively (p<0.044) the conception rate. Group 2 was more successful in maintaining gestation (p<0.025). It is concluded that the three evaluated factors interfere in conception rates and / or gestational loss and should be considered in the genetic improvement programs embryo transfer of the Girolando breed.

References

BÓ, G.A.; BARUSELLI, P.S.; MORENO, D.; CUTAIA, L.; CACCIA, M.; TRÍBULO, R.; TRÍBULO, H.; MAPLETOFT, R.J. The control of folicular wave development for self-appointed embryo transfer programs in cattle. Theriogenology, v.53, p.53-72, 2002.

BÓ, G.A.; MAPLETOFT, R.J. Evaluation and classification of bovine embryos. Animal Reproduction, p.168-173, 2013. Acesso em: 15 de julho de 2019. Disponível em: http://www.cbra.org.br/pages/publicacoes/animalreproduction/issues/download/v10n3/p344-348%20(AR628).pdf.

DANTAS, K.S.A.; CAMPELLO, C.C.; DANTAS, R.A.A.; NUNES, J.F. Seleção de receptoras em um programa de transferência de embriões (PIVE) em bovinos no Nordeste do Brasil. Ciência Animal, v.28, n.1, 2018.

GALLI, C.; CROTTI, G.; NOTARI, C.; TURINI, P.; DUCHI, R.; LAZZARI, G. Embryo production by ovum pick up from live donors. Theriogenology, v.55, p.1341-1357, 2001.

GONÇALVES, P.B.D.; FIGUEIREDO, J.R.; FREITAS, V.J.F. Biotécnicas aplicadas à reprodução animal. 2ª ed., Roca, São Paulo, 2008. 628p.

HANSEN, P.J. Embryonic mortality in cattle from the embryo's perspective. Journal of Animal Science, v.80, n.2, p.33-44, 2002.

HASLER, F.J. The current status of oocyte recovery, in vitro embryo production, and embryo transfer in domestic animals, with an emphasis on the bovine. Journal of Animal Science, v.76, n.3, p.52-74, 1998.

HASLER, J.F. Factors affecting frozen and fresh embryo transfer pregnancy rates in cattle. Theriogenology, v.56, p.1401-14-15, 2001.

HASLER, J.F.; MC CAULEY, A.D.; LATHROP, W.F.; FOOTE, R.H. Effect of donor-embryo-recipient interactions on pregnancy rate in a large-scale bovine embryo transfer program. Theriogenology, v.27, p.139-168, 1987.

STRINGFELLOW, D.A.; SEIDEL, S.M. Manual da Sociedade Internacional de Transferência de Embriões. IETS, 3ª ed., Illinois, p.112-113, 1998.

IETS - The International Embryo Transfer Society. Statistics of embryo collection and transfer on domestic farm. Austrália, 2016. Acesso em: 15 de julho de 2019. Disponível em: https://www.iets.org/pdf/comm_data/IETS_Data_Retrieval_Report_2016_v2.pdf

LONERGAN, P.; FAIR, T. In vitro produced bovine embryos - Dealing with the warts. Theriogenology, v.69, p.17-22, 2008.

LOONEY, C.R.; NELSON, J.S.; SCHNEIDER, H.J.; FORREST, D.W. Improving fertility in beef cow recipients. Theriogenology, v.65, p.201-209, 2016.

MAPA – Ministério da Agricultura, Pecuária e Abastecimento. A agropecuária brasileira contribui para o fortalecimento da nossa economia. Acesso em: 15 de julho de 2019. Acesso em: 15 de julho de 2019. Disponível em: http://www.agricultura.gov.br/agromais/agropecuaria-brasileira.html

ROSA, R.; LIMA, S.C.; ASSUNÇÃO, W.L. Abordagem preliminar das condições climáticas de Uberlândia (MG). Sociedade & Natureza, v.3, n.5, p.91-108, 1991.

SCANAVES, A.L.; CAMPOS, C.C.; SANTOS, R.M. Taxa de prenhez e de perda de gestação em receptoras de embriões bovinos produzidos in vitro. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.65, n.3, p.722-728, 2013.

SILVA, L.A. Taxa de gestação e mortalidade embrionária em receptoras de embriões produzidos in vitro, após sincronização do estro com diferentes protocolos hormonais [dissertação]. Belo Horizonte (Pós-graduação em Ciências Animal), Universidade Federal de Minas Gerais; 2010. 64p. Acesso em: 15 de julho de 2019. Disponível em: http://www.bibliotecadigital.ufmg.br/dspace/bitstream/handle/1843/SMOC-9HDJDA/leandro_disserta__o.pdf?sequence=1.

SWANGCHAN-UTHAI, T.; WALSH, S.W.; ALEXANDER, S.L.H.; CHENG, Z.; CROWE, M.A.; EVANS, A.C.O.; WATHES. D.C. Comparison of mRNA for IGFs and their binding proteins in the oviduct during the peri-oestrous period between dairy heifers and lactating cows. Reproduction, v.142, p.457-465, 2011.

TIEZZI, F.; MALTECCA C.; CECCHINATO, A.; PE NASA, M.; BITTANTE, G. Genetic parameters for fertility of dairy heifers and cows at different parities and relationships with production traits in first lactation. Journal of Dairy Science, v.95, p. 7355-7362, 2012.

TORRES Jr, J.R.S.; PIRES, M.F.A.; SÁ, W.F.; FERREIRA, A.M.; VIANNA, J.H.M.; CAMARGO, L.S.A.; RAMOS, A.A.; FOLHADELLA, I.M.; POLISSENI, J.; FREITAS, C.; CLEMENTE, C.A.A.; SÁ FILHO, M.F.; PAULA-LOPES, F.F.; BARUSELLI, P.S. Effect of maternal heat-stress on folicular growth and oocyte competence in Bosindicus cattle. Theriogenology, v.69, p.155-166, 2008.

VASCONCELOS, J.L.M.; DEMETRIO, D.G.B.; SANTOS, R.M.; CHIARI, J.R.; RODRIGUES, C.A.; SÁ FILHO, O.G. Factors potentially affecting fertility of lactating dairy cow recipients. Theriogenology, v.65, P.192-200, 2006.

VELOSO NETO, H.F.; SILVA, J.C.F.; PEREIRA, L.C.; ANDRADE, J.C.O.; MOURA, M.T.; BARTOLOMEU, C.C.; LIMA, P.F.; OLIVEIRA, M.A.L. Parâmetros que afetam a taxa de prenhez e receptoras bovinas de embriões produzidos in vitro. Medicina Veterinária, Recife, v.8, n.3, p.31-35, 2014.

VIEIRA, L.M.; RODRIGUES, C.A.; MENDANHA, M.F.; SÁ FILHO, M.F.; SALES, J.N.S.; SOUZA, A.H.; SANTOS, J.E.O.; BARUSELLI, P.S. Donor category and seasonal climate associated with embryo production and survival in multiple ovulation and embryo transfer programs in Holstein cattle. Theriogenology, v.82, p.204-2012, 2014.

WOLFENSON, D.; THACHER, W.W.; BANDIGA, L.; SAVI0, J.D.; MEIDAN, R.; LEW, B.J.; BRAW-TAL, R.; BERMAN, A. Effect of heat stress on folicular development during the estrous cycle in lactating dairy cattle. Biology of Reproduction, v.52, p.1106-1113, 1995.

WOLFENSON, D.; INBAR, G.; ROTH, Z.; KAIM, M.; BLOCH, A.; BRAW-TAL R. Follicular dynamics and concentrations of steroids and gonadotropins in lactating cows and nulliparous heifers. Theriogenology, v.62, p.1-42-1055, 2004.

Published

2023-01-27

How to Cite

LOBATO, M. D.; MARQUES, F. L. A.; SOARES, M. M.; FARIA, A. C. F.; CADIMA, G. P.; SANTOS, R. M. dos. Factors influencing the conception rate and pregnancy loss in Girolando embryos produced in vitro. Ciência Animal, [S. l.], v. 29, n. 3, p. 50–58, 2023. Disponível em: https://revistas.uece.br/index.php/cienciaanimal/article/view/10065. Acesso em: 19 jul. 2024.

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