COMPONENTES MOLECULARES ENVOLVIDOS NA CRIOPRESERVAÇÃO DO SÊMEN SUÍNO
Schlagworte:
Espermatozoide, lesões, membrana plasmática, proteínasAbstract
A criopreservação induz mudanças estruturais e funcionais nos espermatozoides, que podem variar entre animais da mesma espécie, mesmo quando o sêmen deles é submetido ao mesmo protocolo de criopreservação. Essa característica leva alguns estudos a questionarem quanto à composição protéica do sêmen e sua influência na proteção espermática, em relação ao choque térmico. O plasma seminal de suínos apresenta predominância de espermadesinas, que são proteínas de baixo peso molecular e apresentam função de proteção da membrana plasmática. A criopreservação é o método mais eficiente para a conservação de espermatozoides a longo prazo. No entanto, o uso do sêmen congelado na espécie suína ainda está associado a baixos índices produtivos, pois o processo de criopreservação ocasiona diversos danos ao espermatozoide. Estudos têm sido realizados com a finalidade de identificar as causas específicas desses danos, a fim de promover uma melhor proteção ao espermatozoide criopreservado. Dessa forma, o presente trabalho tem como objetivo abordar os principais componentes moleculares do sêmen, que estão envolvidos no processo de criopreservação.
Literaturhinweise
ALBERTS, B.; JOHNSON, A.; LEWIS, J.; MORGAN, D.; RAFF, M.; ROBERTS, K.; WALTER, P.; WILSON, J.; HUNT, T. Organização interna da célula: Estrutura da membrana. In: ___. Biologia Molecular da Célula. 4ª ed., Porto Alegre: Artmed. Cap. 10, p.583-595, 2004.
ALMEIDA, J.L. Efeito de diferentes concentrações de plasma seminal na criopreservação do sêmen equino. 2006. 75p. (Dissertação). Brasília: Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília; 2006.
AMANN, R.P.; GRAHAM, J.K. Spermatozoa function. In: McKINNON, A.O.; VOSS, J.L. (Ed.) Equine Reproduction. 2a ed., Philadelphia: Lea &Febiger, p.715-745, 1993.
ANDRADE, A.F.C.; PEDROSA, A.C.; PASSARELLI, M.S.; MARTINS, S.M.M.K. Protocolos e possibilidades de criopreservação de sêmen suíno. Revista Brasileira de Reprodução Animal, v.43, n.2, p.89-96, 2019.
AZEVEDO, H.C.; MACHADO, R.; SIMPLÍCIO, A.A.; SOARES, A.T. Características do sêmen caprino congelado: influência do tipo de palheta e concentração espermática. Revista Ciência Rural, v.5, n.2, p.148-157, 2000.
BAILEY, J.L.; LESSARD, C.; JACQUES, J.; BRÈQUE, C.; DOBRINSKI, I.; ZENG, W.; GALANTINO-HOMER, H.L. Cryopreservation of boar semen and its future importance to the industry. Theriogenology, v.70, p.1251–1259, 2008.
BOERKE, A.; DIELEMAN, S.J.; GADELLA, B.M.A. Possible role for sperm RNA in early embryo development.Theriogenology, v.68, n.1, p.147–155,2007.
BORTOLOZZO, F.P.; WENTZ, I.; BENNEMANN, P.E.; BERNADI, M.L.; WOLLMANN, E.B.; FERREIRA, F.M.; NETO, G.B. Inseminação artificial na suinocultura tecnificada. Suinocultura em ação: Copyright, Porto Alegre, Brasil, 2005. 85p.
BUHR, M.M.; CURTIS, E.F.; KAKUDA, N.S. Composition and behavior of head membrane lipids of fresh and cryopreserved boar sperm. Cryobiology, v.31, p.224-238, 1994.
CABALLERO, I.; VAZQUEZ,J.M.; GARCÍA, E.M.; PARRILLA,I.; ROCA,J.; CALVETE, J.J.; SANZ, L.; MARTÍNEZ, E.A. Major proteins of boar seminal plasma as a tool for biotechnological preservation of spermatozoa. Theriogenology, v.70, n.8, p.1352–1355, 2008.
CALVETE, J.J.; REINERT, M.; SANZ, L.; TÖPFER-PETERSEN, E. Effect of glycosylation on the heparin-binding capability of boar and stallion seminal plasma proteins. Journal of Chromatography, v.711, n.1, p.167–173, 1995.
CALVETE, J.J.; SOLIS, D.; SANZ, L.; DIAZ-MURILLO, T.; SCHAFER, W.; MANN, K.; TÖPFER-PETERSEN, E. Characterization of two glycosylated boar spermadhesins. European Journal of Biochemistry, v.218, p.719-725, 1993.
CASAS, I.; FLORES, E. Gene banking: the freezing strategy. In: BONET, S.; CASAS, I.; HOLT, W.V.; YESTE, M.Editors. Boar Reproduction. Berlin: Springer, p.551–88, 2013.
CASAS, I.; SANCHO, S.; BRIZ, M.; PINART, E.; BUSSALLEU, E.; YESTE, M. Freezability prediction of boar ejaculates assessed by functional sperm parameters and sperm proteins. Theriogenology, v.72, n.7, p.930–948, 2009.
CENTURIÓN, F.; VÁZQUEZ, J. M.; CALVETE, J. J.; ROCA, J.; SANZ, L.; PARRILLA, I.; GARCÍA, E. M.; MARTÍNEZ, E.A. Influence of porcine spermadhesins on the susceptibility of boar spermatozoa to high dilution. Biology of Reproduction, v.69, n.3, p.640–646, 2003.
CHUAYCHU-NOO, N.; THANANURAK, P.; CHANKITISAKUL, V.; VONGPRALU, T. Supplementing rooster sperm with Cholesterol-Loaded-Cyclodextrin improves fertility after cryopreservation. Cryobiology, v.74, p.8-12, 2017.
COLLARES, T.; BONGALHARDO, D.C.;DESCHAMPS, J.C.; MOREIRA, H.L.M. Transgenic animals: The melding of molecular biology and animal reproduction. Animal Reproduction, v.2, n.1, p.11-27, 2005.
CORCINI, C.D.; VARELA, A.S.; PIGOZZO, R.; RAMBO, G.; GOULARTE, K.L.; CALDERAM, K.; LEON, P.M.M.; BONGALHARDO, D.C.; LUCIA JR., T. Pre-freezing and post-thawing quality of boar sperm for distinct portions of the ejaculate and as a function of protein bands presente in seminal plasma. Livestock Science, v.145, n.1, p.28-33, 2012.
DE LEEUW, F.; COLENBRANDER, B.; VERKLEIJ, A. The role membrane damage plays in cold shock and freezing injury. Reproduction in Domestic Animals, v.1, n.1, p.95–104, 1990.
DE JONGE, C.; BARRATT, C.L. Gamete donation: a question of anonymity. Fertility and Sterility, v.85, n.2, p.500-501, 2006.
DU, J.; SHEN, J.; WANG, Y.; PAN, C.; PANG, W.; DIAO, H.; DONG, W. Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane. Oncotarget, v.7, n.37, p.58832–58847, 2016.
EDDY, E. M.; O’BRIEN, D. A. The spermatozoon. In: KNOBIL, E.; NEILL, J. D. The Physiology of Reproduction. 2a ed., New York: Raven Press. Cap. 2, p.29-77, 1994.
EKHALASI-HUNDRIESER, M.; SCHAFER, B.; KIRCHHOFF, C.; HESS, O.; BELLAIR, S.; MÜLLER, P.; TÖPFER- PETERSEN, E. Structural and molecular characterization of equine sperm-binding fibronectin-II module proteins. Molecular Reproduction Development, v.70, n.1, p.45–57, 2005.
EVANS, G., MAXWELL, W.M C. Salamoninseminación artificial de ovejas y cabras. 1a ed., Zaragoza: Acribia, 1990. 191p.
FLESCH, F.M.; GADELLA, B.M. Dynamics of the mammalian sperm plasma membrane in the process of fertilization.BiochimicaetBiophysica Acta, v.1469, n.3, p.197-235, 2000.
FOXCROFT, G.R.; DYCK, M.K.; RUIZ-SANCHEZ, A.; NOVAK, S.; DIXON, W.T. Identifying useable semen. Theriogenology, v.70, n.8, p.1324–36,2008.
GADELLA, B.M.; COLENBRANDER, B. Bicarbonate dependent capacitation of mammalian sperm cells: a comparative overview. In: Proceedings of a workshop on transporting gametes and embryos, 12, Brewster – Massachusetts, p.43-48, 2003.
GAO, D.; CRITSER, J.K. Mechanisms of cryoinjury in living cells. ILAR Journal, v.41, n.4, p.187–196, 2000.
GARCIA, E.M.; VÁZQUEZ, J.M.; CALVETE, J.J.; SANZ, L.; CABALLERO, I.; PARRILLA, I.; GIL, M.A.; ROCA, J.; MARTINEZ, E.A. Dissecting the protective effect of the seminal plasma spermadhesin PSP-I/PSP-II on boar sperm functionality. Journal of Andrology, v.27, n.3, p.434-443, 2006.
GÓMEZ-FERNÁNDEZ, J.; GÓMEZ-IZQUIERDO, E.; TOMÁS, C.; MOCÉ, E.; DE MERCADO, E. Is sperm freezability related to the post-thaw lipid peroxidation and the formation of reactive oxygen species in boars? Reproduction in Domestic Animals, v.48, n.2m p.177–82, 2013.
GRAHAM, J.K. Cryopreservation of stallion spermatozoa. Veterinary Clinics of North America: Equine Practice, v.12, n.1, p.131-147, 1996.
GUIMARÃES, D.B.; BARROS, T.B.; VAN TILBURG, M.F.; MARTINS, J.A.M.; MOURA, A.A.; MORENO, F.B.; MONTEIRO-MOREIRA, A.C.; MOREIRA, R.A.; TONIOLLI, R. Sperm membrane proteins associated with the boar semen cryopreservation. Animal Reproduction Science, v.183, p.27-38, 2017.
HAFEZ, E.S.E.; HAFEZ, B. Reprodução Animal. 7ª ed., Manole: São Paulo, 2003. 530p.
HAFEZ, E.S.E. Preservação e criopreservação de gametas e embriões. In: __. Reprodução Animal. 6a ed.,Manole: São Paulo. Cap. 24, p.513-535, 1995.
HAMMERSTEDT, R.H.; GRAHAM, J.K.; NOLAN, J.P. Cryopreservation of mammalian sperm; what we ask them to survive. Journal of Andrology, v.11, n.1, p.73-88, 1990.
HOLT, W.V.; MEDRANO, A.; THURSTON, L.M.; WATSON, P.F. The significance of cooling rates and animal variability for boar sperm cryopreservation: insights from the cryomicroscope. Theriogenology, v.63, n.2, p.370–382, 2005.
HOLT, W.V.; NORTH, R.D. Determination of liquid components and thermal phase transition temperature in an enriched plasma membrane fraction from spermatozoa. Journal of Reproduction and Fertility, v.73, p.285-294, 1985.
JAISWAL, B.S.; CONTI, M. Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase. Journal of Biology and Chemestry, v.276, n.35, p.31.698–31.708, 2001.
JOHNSON, L.A.; WEITZE, K.F.; FISER, P.; MAXWELL, W.M. Storage of boar semen. Animal Reproduction Science, v.62, n.1-3, p.143-172, 2000.
KANG, S.; PANG, W-K.; RYU, D.-Y.; SONG, W.-H.; RAHMAN, M.S.; PARK, Y.-J.;PANG, M.-G. Porcine seminal protein-I and II mRNA expression in boar spermatozoa is significantly correlated with fertility. Theriogenology, v.138, n.1, p.31-38, 2019.
KASIMANICKAM, R.; KASIMANICKAM, V.; THATCHER, C.D.; NEBEL, R.L; CASSELL B.G. Relationships among lipid peroxidation, glutathione peroxidase, superoxide dismutase, sperm parameters, and competitive index in dairy bulls. Theriogenology, v.67, n.7, p.1004–1012, 2007.
KOPEIKA, J.; THORNHILL, A.; KHALAF, Y. The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence. Humain Reproduction Update, v.30, n.1, p.209–227, 2015.
KOTHARI, S.; THOMPSON, A.; AGARWAL, A.; DU PLESSIS, S.S. Free radicals: their beneficial and de trimental effects on sperm function. Indian Journalof Experimental Biology, v.48, p.425-435, 2010.
LEE, Y.S.; LEE, S.; LEE, S.H.; YANG, B.K.; PARK, C.K. Effect of cholesterol-loaded-cyclodextrin on sperm viability and acrosome reaction in boar semen cryopreservation. Animal Reproduction Science, v.159, p.124-130, 2015.
LEHNINGER, A.L.; NELSON, D.L.; COX, M.M. Lehninger: Princípios de Bioquímica. 4ª ed. São Paulo: Editora Sarvier, 2005. 1120p.
LI, J.; ROCA, J.; PÉREZ-PATIÑO, C.; BARRANCO, I.; MARTINEZ, E.A.; RODRIGUEZ-MARTINEZ, H.; PARRILLA, I. Is boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma? Animal Reproduction Science, v.195, p.30-37, 2018.
LONE, S.A. Possible mechanisms of cholesterol-loaded cyclodextrin action on sperm during cryopreservation. Animal Reproduction Science, v.192, p.1-5, 2018.
MACK, S.R.; EVERINGHAM, J.; ZANEVELD, L.J.D. Isolation and partial characterization of the plasma membrane from human spermatozoa. Journal of Experimental Zoology, v.240, n.1, p.125-136, 1986.
MEDEIROS, C.M.O.; FORELL, F.; OLIVEIRA, A.T.D.; RODRIGUES, J.L. Current status of sperm cryopreservation: why isn't it better? Theriogenology, v.57, n.1, p.327-344, 2002.
MEDRANO, A.; HOLT, W.V.; WATSON, P.F. Controlled freezing studies on boar sperm cryopreservation. Journal of Andrology, v.41, n.4, p.246–250, 2009.
MOCÉ, E.; GRAHAM, J.K. Cholesterol-loaded cyclodextrins added to fresh bull ejaculates improve sperm cryosurvival. Journal of Animal Science, v.84, n.5, p.826–833, 2006.
MOURA, A.A.; CHAPMAN, D.A.; KOC, H.; KILLIAN, G.J. A comprehensive proteomic analysis of the accessory sex gland fluid from mature Holstein bulls. Animal Reproduction Science, v.98, n.3-4, p.169-188, 2007.
MORAN, D.M.; JASKO, D.J.; SQUIRES, E.L.; AMANN, R.P. Determination of temperature and cooling rate which induce cold shock in stallion spermatozoa. Theriogenology, v.38, p.999-1012, 1992.
MUKAI, C.; OKUNO, M. Glycolysis plays a major role for adenosine triphosphate supplementation in mouse sperm flagellar movement. Biology of Reproduction, v.71, p.540–547, 2004.
NEILLL, D.J. Physiology of Reproduction. 3a ed., vol. 1, 2006. 3296p.
NESCI, S.; SPINACI, M.; GALEATI, G.; NEROZZI, C.; PAGLIARANI, A.; ALGIERI, C.; TAMANINI, C.; BUCCI, D. Sperm function and mitochondrial activity: An insight on boar sperm metabolism. Theriogenology, v.144, p.82-88, 2020.
NOVAK, S.; RUIZ-SÁNCHEZ, A.; DIXON, W.T.; FOXCROFT, G.R.; DYCK, M.K. Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars. Journal of Andrology, v.31, n.2, p.188–200, 2010.
PARKS, J.E.; GRAHAM, J.K. Effects of cryopreservation procedures on sperm membranes. Theriogenology, v.38, p.209-222, 1992.
PAOLI, D.; LOMBARDO, F.; LENZI, A.; GANDINI, L. Sperm cryopreservation: effects on chromatin structure. Advances in Experimental Medicine and Biology, v.791, p.137–150, 2014.
PEÑA, F.J.; DAVILA, M. P.; BALL, B.A.; SQUIRES, E.L.; MARTIN MUÑOZ, P, FERRUSOLA, C.O.; SILVA, C.B.The impact of reproductive technologies on stallion mitochondrial function. Reproduction in Domestic Animals, v.50, p.529–37, 2015.
PEÑA, F.J.; SARAVIA, F.; NÚÑEZ-MARTÍNEZ, I.; JOHANNISSON, A.; WALLGREN, M.; RODRÍGUEZ MARTÍNEZ, H. Different portions of the boar ejaculate vary in their ability to sustain cryopreservation? Animal Reproduction Science, v.93, p.101–113, 2006.
PEÑA, F.J.; JOHANNISSON, A.; WALLGREN, M. Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. Animal Reproduction Science, v.78, n.1-2, p.85-98, 2003.
PRIETO‐MARTÍNEZ, N.; VILAGRAN, I.; MORATÓ, R.; ÁLAMO, M.M.R.; RODRÍGUEZ‐GIL, J.E.; BONET, S.; YESTE, .M. Relationship of aquaporins 3 (AQP3), 7 (AQP7), and 11 (AQP11) with boar sperm resilience to withstand freeze–thawing procedures. Andrology, v.5, n.6, p.1153-1164, 2017.
RATH, D.; BATHGATE, R.; RODRÍGUEZ-MARTÍNEZ, H.; ROCA, J.; STRZEZEK, J.; WABERSKI, D. Recent advances in boar semen cryopreservation Society of Reproduction and Fertility, v.66, Suppl. 1, p.51–66, 2009.
RECUERO, S.; FERNANDEZ-FUERTES, B.; BONET, S.; BARRANCO, I.; YESTE, M. Potential of seminal plasma to improve the fertility of frozen-thawed boar spermatozoa. Theriogenology, v.137, p.36-42, 2019.
ROCA, J.; HERNANDEZ, M.; CARVAJAL, G.; VAZQUEZ, J.M.; MARTINEZ, E.A. Factors influencing boar sperm cryosurvival. Journal of Animal Science, v.84, n.10, p.2692–2699, 2006.
RODRÍGUEZ-MARTÍNEZ, H.; SARAVIA, F.; WALLGREN, M.; TIENTHAI, P.; JOHANNISSON, A.; VÁZQUEZ, J.M.; MARTÍNEZ, E.; ROCA, J.; SANZ, L.; CALVETE, J.J. Boar spermatozoa in the oviduct. Theriogenology, v.63, p.514-535, 2005.
RODRIGUEZ-MARTINEZ, H.; IBORRA, A.; MARTINEZ, P.; CALVETE, J.J. Immunoelectron microscopic imaging of spermadhesin AWN epitopes on boar spermatozoa bound in vivo to the zona pellucida. Reproduction Fertility Development, v.10, p.491–497, 1998.
ROZEBOOM, K.J.; TROEDSSON, M.H.T.; HODSON, H.H.; SHURSON, G.C.; CRABO, B.G. The importance of seminal plasma on the fertility of subsequent artificial insemination in swine. Journal of Animal Science, v.78, n.2, p.443–448, 2000.
SALAMON, S.; MAXWELL, W.M.C. Storage of ram semen. Animal Reproduction Science, v.62, n.1-3, p.77-111, 2000.
SALMON, V.M.; CASTONGUAY, F.; DEMERS-CARON, V.; LECLERC, P.; BAILEY, J.L. Cholesterol-loaded cyclodextrin improves ram sperm cryoresistance in skim milk-extender. Animal Reproduction Science, v.177, n.1, p.1-11, 2017.
SILVA, E.F.; CARDOSO, T.F.; TAVARES, G.C.; COSTA, V.G.G.; SILVA, J.F.; VARELA, A.S.JR.; LEITE, F.P.L.; CORCINI, C.D. Criopreservação de sêmen suíno e a pesquisa com antioxidantes. Revista Portuguesa de CiênciasVeterinárias, v.108, p.103-112, 2013.
STRZEŻEK, J.; WYSOCKI, P.; KORDAN, W.; KUKLIŃSKA, M.; MOGIELNICKA, M.; SOLIWODA, D.; FRASER, L. Proteomics of boar seminal plasma – current studies and possibility of their application in biotechnology of animal reproduction. Reproduction and Biology, v.5, p.279-290, 2005.
STRZEZEK, J. Secretory Activity of Boar Seminal Vesicle Glands. Reproductive Biology, v.2, n.3, p.243–66, 2002.
THURSTON, L.M.; SIGGINS, K.; MILEHAM, A.J.; WATSON, P.F.; HOLT, W.V. Semen cryopreservation: a genetic explanation for species and individual variation? Cryo Letters, v.23, n.4, p.255–262, 2002.
TONIOLLI, R.; GUIMARÃES, D.B.; BARROS, T.B. Proteínas do sêmen e sua relação com a resistência à congelação em ejaculados de diferentes varrões. Revista Brasileira de Reprodução Animal, v.41, n.1, p.297-311, 2017.
TROEDSSON, M.H.; DESVOUSGES, A.; ALGHAMDI, A.S.; DAHMS, B.; DOW, C.A.; HAYNA, J.; VALESCO, R.; COLLAHAN, P.T.; MACPHERSON, M.L.; POZOR, M.; BUHI, W.C. Components in seminal plasma regulating sperm transport and elimination. Animal Reproduction Science, v.89, n.1-4, p.171–86, 2005.
TURNER, R.M. Tales from the tail: what do we really know about sperm motility? Journal of Andrology, v.24, n.6, p.790-802, 2003.
VADNAIS, M.L.; ALTHOUSE, G.C. Characterization of capacitation, cryoinjury, and the role of seminal plasma in porcine sperm. Theriogenology, v.76, p.1508–16, 2011.
VALENCIA, J.; GÓMEZ, G.; LÓPEZ, W.; MESA, H.; HENAO, F.J. Relationship between HSP90a, NPC2 and L-PGDS proteins to boar semen freezability. Journal of Animal Science and Biotechnology, v.8, p.1-10, article 21, 2017.
VILAGRAN, I.; YESTE, M.; SANCHO, S.; CASAS, I.;RIVERA DEL ALAMO, M.M.; BONET, S. Relationship of sperm small heat-shock protein 10 (ODF1/HSPB10) and voltage-dependent anion channel 2 (VDAC2) with semen freezability in boars. Theriogenology, v.82, p.418–426, 2014.
WATERHOUSE, K.E.; HOFMO, P.O.; TVERDAL, A.; MILLER JR, R.R. Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm. Reproduction, v.131, n.5, p.887–894, 2006.
WATSON, P.F. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reproduction Fertility and Development, v.7, n.4, p.871–891, 1995.
YESTE, M. State-of-the-art of boar sperm preservation in liquid and frozen state. Animal Reproduction, v.14, n.1, p.69-81, 2017.
