ANÁLISE BIOQUÍMICA DOS CONSTITUINTES ORGÂNICOS E INORGÂNICOS DO PLASMA SEMINAL DE CUTIAS

Autores

  • Ana Glória Pereira Laboratório de Conservação de Germoplasma Animal da Universidade Federal Rural do Semi-Árido(UFERSA)
  • Maiko Roberto Tavares Dantas Laboratório de Conservação de Germoplasma Animal da Universidade Federal Rural do Semi-Árido(UFERSA)
  • Samara Sandy Jerônimo Moreira Laboratório de Conservação de Germoplasma Animal da Universidade Federal Rural do Semi-Árido(UFERSA)
  • Náyra Rachel Nascimento Luz Laboratório de Conservação de Germoplasma Animal da Universidade Federal Rural do Semi-Árido(UFERSA)
  • Alexandre Rodrigues Silva Laboratório de Conservação de Germoplasma Animal da Universidade Federal Rural do Semi-Árido(UFERSA)

Palavras-chave:

Conservation, Semiarid, Rodents, Reproduction

Resumo

The objective of the present study was to identify the biochemical components of the agoutis’ seminal plasma. For this purpose, six adult males were collected by means of electroejaculation. Biochemical analysis was performed using commercial kits and the absorbances were read on a spectrophotometer. The results were described as mean and standard error. The following organic constituents were identified in the seminal plasma: albumin (6.64 ±2.31g/dL), total proteins (1.9±0.62g/dL), glucose (26.27±9.84mg/dL), fructose (26.92±8.08mg/dL), citric acid (408.28±227.92mg/dL)), triglycerides (282.04±83.58mg/dL) and cholesterol (125.16±34.35mg/dL). Regarding inorganic components, chlorides (283.66±104,11mEq/L), magnesium (4.24±0.38mg/dL), phosphorus (3.67±0.59mg/dL), calcium (12.47±1.85mg/dL), and iron (620.63±266.33µg/dL). It should be noted that this is the first description of the biochemical composition of seminal plasma in the species Dasyprocta leporina. This information will be useful for the improvement of sperm conservation protocols for the species.

Referências

ABOU-SHA, F.R.; WARD, N.I.; EVERARD, D.M. The role of trace elements in male infertility. Fertility And Sterility,v. 2, n.2, p.307-310, 1989.

BAAS, E.J.; POTKAY, S.; BACHER, J.D. The agouti (Dasyprocta sp) in biomedical research and captivity. Laboratory Animal Science, v.26, n.5, p.788-796, 1976.

CASTELO, T.S.; SILVA, A.R. Eletroejaculação em mamíferos silvestres: principais fatores que afetam sua eficiência. RevistaBrasileira de Reprodução Animal, v.38, n.4, p.208-213, 2015.

ELZANATY, S.; RICHTHOFF, J.; MALM, J.; GIWERCMAN, A. The impact of epididymal and accessory sex gland function on sperm motility. HumanReproduction, v.17, n.11, p.2904–11,2002.

FERREIRA, J.B.F.; PIO, C.H.S.; LIMA, E.S. A dosagem da ferritina no plasma seminal pode ser um marcador do estresse oxidativo no sêmen? Newslab, v.94, n.1, p.170-176, 2009.

KOZIOROWSKA-GILUN, M.; KOZIOROWSKI, M.; STRZEZEK, J.; FRASER L. Seasonal changes in antioxidant defense systems in seminal plasma and fluids of boar reproductive tract. Reproductive Biology, v.11, n.1, p.37-47,2011.

MANN, T. Biochemistry of semen and of the male reproductive tract. 2ª ed.,London: Methuen, 1964. 493p.

MOLLINEAU, W.M.; ADOGWA, A, O.; GARCIA, G.W. Improving the efficiency of the preliminary electroejaculation technique developed for semen collection from the agouti (Dasyprocta leporina). Journal of Zoo and Wildlife Medicine, v.41,n.4, p.633-637, 2010.

MURASE, T.; IMAEDA, N.; YAMADA H.; MIYAZAWA K. Seasonal changes in semen characteristics, composition of seminal plasma and frequency of acrosome reaction induced by calcium and calcium ionophore A23187 in Large White boars. Journal of Reproduction and Development, v.53, n.4, p.853-865, 2007.

SOFIKITIS, N.; MIYAGAWA, I. Secretory dysfunction of the male accessory genital glands due to prostatic infections and fertility: a selected review of the literature. Japanese Journal of Fertiligy and Sterility, v.36, p.690-699, 1991.

WONG, W.Y.; FLIK, G.; GROENEN, P.M.W.; SWINKELS, D.W.; THOMAS, C.M.G.; COPIUS-PEEREBOOM, J.H.J.; MERKUS, H.M.W.M.; STEEGERS-THEUNISSEN, R.P.M. The impact of calcium, magnesium, zinc, and copper in blood and seminal plasma on semen parameters in men. Reproductive Toxicology, v.15, n.20 p.131-136, 2001.

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Publicado

2021-12-31

Como Citar

PEREIRA, A. G. .; DANTAS, M. R. T. .; MOREIRA, S. S. J. .; LUZ, N. R. N. .; SILVA, A. R. . ANÁLISE BIOQUÍMICA DOS CONSTITUINTES ORGÂNICOS E INORGÂNICOS DO PLASMA SEMINAL DE CUTIAS. Ciência Animal, [S. l.], v. 31, n. 1, p. 25–28, 2021. Disponível em: https://revistas.uece.br/index.php/cienciaanimal/article/view/9555. Acesso em: 21 maio. 2024.

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Resumo Expandido - Artigos Originais