EVALUATION OF A MICROIMMUNODIFUSION ASSAY FOR THE DIAGNOSIS OF ENZOOTIC BOVINE LEUKOSIS
Hamilton Pereira SANTOS- Roberto Soares de CASTRO
- Sérgio Alves do NASCIMENTO
- Nancyleni Pinto Chaves BEZERRA
- Danilo Cutrim BEZERRA
- Lisa Hauane de Melo SANTANA
- Helder de Moraes PEREIRA
Publicado 2023-10-10
Palavras-chave
- Bovino,
- Macro-IDGA,
- EBL,
- Antígeno
Como Citar
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Resumo
This work was developed to evaluate how a micro-AGID using simple protocol for obtaining the antigen compared to a macro-AGID. A total of 450 serum samples from 92 herds in 23 counties that make up the dairy herd of Maranhão were used. The antigen used in micro-AGID was obtained by desalting of supernatant of FLK cells infected with BLV against the polyethylenoglicol. In micro-AGID 10µL of antigen and positive serum control was used and 30 µl of test serum, in the macro-AGID 25 µl of all reagents were used. Of the compared sera, 57.56% (n=259) and 54.44% (n=245) showed positive animals reagents results in micro-AGID and macro-AGID, respectively. There was a very good agreement between both techniques (K=0.91), with sensitivity and specificity of the macro-AGID for micro-AGID of 93.43% and 98.43% with an accuracy of 95.96%. Micro-AGID showed clearer lines than those observed in the macro-AGID and reading can be made 24 hours before the macro-AGID. It is concluded that micro-AGID can be used successfully in the serological diagnosis of EBL, with the advantage of greater speed in issuing the results and obtaining the antigen with a simple technique.
Downloads
Referências
- ALTANER, C.; ZAJAC, V.; BÁN, J. A simple and inexpensive metod for detection of BLB infected cattle based on modified ELISA principal. Zentralblatt für Vetemärmedizin, v.29, n.8, p.583-590, 1982.
- ARRUDA, E.; OLIVEIRA, M.; NASCIMETO, S.; CASTRO, R. Avaliação de uma microimunodifusão em gel de ágar para diagnóstico de Lentivírus de pequenos ruminantes (LVPR) em caprinos. Ciência Animal Brasileira, v.1, n.3, p.560-565, 2011.
- ASTUDILLO, V.M.; KANTOR, I.N. El problema de la validez de una prueba diagnostica para uso masivo como procedimento estatístico de clasificación. Boletin Centro Panamericano Fiebre Aftosa, v.43/44, n.1, p.37-43, 1981.
- BRAGA, F.M.; VAN DER LAAN, C.W.; HALFEN, D.C.; VIDOR, T. Avaliação de métodos de controle da infecção pelo vírus da leucose enzoótica bovina. Ciência Rural, v.27, n.4, p.635-640, 1997.
- BRAGA, F.M.; VAN DER LAAN, C.W.; SCHUCH, L.F.; HALFEN, D.C. Infecção pelo vírus da Leucose Enzoótica Bovina. Ciência Rural, v.28, n.1, p.163-72, 1998.
- FETROW, J.; FERRE, J.F. Bovine leukemia virus infection and mastitis. Journal Dairy Science, v.65, n. 05, p.881-882,1982.
- FILHO, L.C.F.B.; FERNANDES, A.C.C.; SILVA, T.I.B.; RAMOS, T.R.R.; MELO, L.E.H. Performance assessment of imported ELISA in the serodiagnosis of the enzootic bovine leukosis in herds of Pernambuco state, Brazil. Arquivo do Instituto Biológico, v.86, n.1, p.1-4, 2019.
- GONZÁLEZ, T.; LICURSI, M.; BONZO, E. Leucose Bovina Enzoótica: Desempenho de um ELISA indireto empregado no diagnóstico sorológico. Brazilian Journal of Microbiology, v.38, n.1, p.1-5, 2007.
- JOHNSON, R.; KANEENE, J. Bovine leukemia virus and enzootic bovine leukosis. Veterinary Bull, v.62, n.3, p.287-314, 1992.
- KORNIIENKO, L.Y.; PYSKUN, A.V.; TSARENKO, T.M.; UKHOVSKYI, V.V.; KYIVSKA, G.V.; MOROZ, O.A.; KOVALENKO, V.L.; ALIEKSEIEVA, G.B. Retrospective analysis of the epizootic situation of enzootic bovine leukosis in Ukraine in 1994–2019. Regulatory Mechanisms in Biosystems, v.11, n.3, p.372-377, 2020.
- MEIRELLES, C.; DITTRICH, T.; CIPRIANO, F.; OLLHOFF, R.D. Evolution of Enzootic Bovine Leukosis prevalence in a university dairy herd. Semina: Ciências Agrárias, v.30, n.1, p.671-678, 2009.
- MILLER, J.; VAN DER MAATEN, M. Sorologic detection of Bovine Leukemia Virus infection. Veterinary Microbiololy, v.31, n.6, p.47-55, 1976.
- MILLER, J.M.; MILLER, L.D.; OLSON, C.; GILLETE, K.G. Virus-like particles in phytohemaggIutinin-stimulate lymphocyte cultures with reference to bovine lymphosarcoma. Journal of the National Cancer Institute, v.43, p.1297-1305, 1969. Disponível em: http://ictvonline.org/virusTaxonomy.asp. Acesso em: 01 jan. 2022
- OIE. “Enzootic Bovine Leukosis” in the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 2018. Disponível em: https://www.woah.org/Terrestrial Manual. Acesso em: 01 jan. 2022.
- ONUMA, M.; OLSON, C.; BAUMGARTENER, L.E.; PEARSON, L.D. An ether sensitive antigen associated with bovine leukemia virus infection. Journal of the National Cancer Institute, v.55, n.5, p.1155-1158, 1975.
- PAGE, M.; THORPE, R. Methods in Molecular Biology: Immunochemical Protocols. 2. ed. Pound JD (Totowa, NJ: Humana Press), 1998.
- SANTOS, H.P.; PEREIRA, H.M.; NASCIMENTO, S.A.; COUTINHO, L.C.A.; TEIXEIRA, W.C.; ARRUDA, R.C.N.; BEZERRA, N.P.C.; BEZERRA, D.C.; CASTRO, R.S. Frequência de anticorpos e fatores de risco associados à leucose enzoótica bovina em rebanhos da bacia leiteira do estado do Maranhão. Arquivos do Instituto Biológico, v.78, n.3, p.351-358, 2011.
- TÎRZIU, E.; CUMPĂNĂȘOIU, C.; NICHITA, I.; REMAN, G.D.; SONEA, C.; SERES, M. Performance assessment of three tests applied in enzootic bovine leukosis diagnosis. Romanian Biotechnological Letters, v.19, n.5, p.9666-9677, 2014.
- VAN DER MAATEN, M.; MILLER, J.M.; BOOTHE, A.D. Replicating type-C virus particles in monolayer cell cultures of tissues from cattle with lymphosarcoma. Journal of the National Cancer Institute, v.52, n.2, p.491-497., 1974.
- WINWARD, L.D.; LEENDERTSEN, L.; SHEN, D.T. Microimmunodiffusion test for diagnosis of ovine progressive pneumonia. American Journal of Veterinary Research, v.40, n.7, p.564-6, 1979.